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Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer

BACKGROUND: The purpose of our research was to determine the correlation of amplification, protein expression and somatic mutation of c-MET in IIIb-IV stage NSCLC (Non-small cell lung cancer). We also explored correlation of c-MET variation with clinical outcome. RESULTS: c-MET expression was observ...

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Autores principales: Duan, Jianchun, Yang, Xiaodan, Zhao, Jun, Zhuo, Minglei, Wang, Zhijie, An, Tongtong, Bai, Hua, Wang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788667/
https://www.ncbi.nlm.nih.gov/pubmed/29416799
http://dx.doi.org/10.18632/oncotarget.23474
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author Duan, Jianchun
Yang, Xiaodan
Zhao, Jun
Zhuo, Minglei
Wang, Zhijie
An, Tongtong
Bai, Hua
Wang, Jie
author_facet Duan, Jianchun
Yang, Xiaodan
Zhao, Jun
Zhuo, Minglei
Wang, Zhijie
An, Tongtong
Bai, Hua
Wang, Jie
author_sort Duan, Jianchun
collection PubMed
description BACKGROUND: The purpose of our research was to determine the correlation of amplification, protein expression and somatic mutation of c-MET in IIIb-IV stage NSCLC (Non-small cell lung cancer). We also explored correlation of c-MET variation with clinical outcome. RESULTS: c-MET expression was observed in 28.6% (56/196) cases, and among those 13.8% (27/196) were shown to be FISH positive. Only 2.67% patients in this study carried the c-MET mutation. Cases with c-MET FISH positive were all IHC positive ,but in IHC positive cases, only half were FISH positive. Among patients with IHC(2+) staining, 35.5% was FISH positive, while cases with IHC(3+) staining,64% was FISH positive. Both protein expression and copy number of c-MET did not significantly correlate with clinical prognosis in these patients treated with EGFR-TKIs. CONCLUSIONS: IHC could be used as a preliminary screening method for c-MET copy number amplification and should be confirmed by FISH only in IHC positive case which facilitate selection of ALK or MET inhibitor therapy. METHODS: c-MET gene copy number, protein expression and somatic mutation for exon 14 were detected by fluorescent- In-Situ-Hybridization (FISH), Immunohistochemistry (IHC), and Denaturing-High-Performance-Liquid-Chromatography (DHPLC), respectively, in 196 NSCLC patients. The relationship between c-MET abnormalities and clinical outcome of targeted therapy was analyzed by McNemar's test.
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spelling pubmed-57886672018-02-07 Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer Duan, Jianchun Yang, Xiaodan Zhao, Jun Zhuo, Minglei Wang, Zhijie An, Tongtong Bai, Hua Wang, Jie Oncotarget Research Paper BACKGROUND: The purpose of our research was to determine the correlation of amplification, protein expression and somatic mutation of c-MET in IIIb-IV stage NSCLC (Non-small cell lung cancer). We also explored correlation of c-MET variation with clinical outcome. RESULTS: c-MET expression was observed in 28.6% (56/196) cases, and among those 13.8% (27/196) were shown to be FISH positive. Only 2.67% patients in this study carried the c-MET mutation. Cases with c-MET FISH positive were all IHC positive ,but in IHC positive cases, only half were FISH positive. Among patients with IHC(2+) staining, 35.5% was FISH positive, while cases with IHC(3+) staining,64% was FISH positive. Both protein expression and copy number of c-MET did not significantly correlate with clinical prognosis in these patients treated with EGFR-TKIs. CONCLUSIONS: IHC could be used as a preliminary screening method for c-MET copy number amplification and should be confirmed by FISH only in IHC positive case which facilitate selection of ALK or MET inhibitor therapy. METHODS: c-MET gene copy number, protein expression and somatic mutation for exon 14 were detected by fluorescent- In-Situ-Hybridization (FISH), Immunohistochemistry (IHC), and Denaturing-High-Performance-Liquid-Chromatography (DHPLC), respectively, in 196 NSCLC patients. The relationship between c-MET abnormalities and clinical outcome of targeted therapy was analyzed by McNemar's test. Impact Journals LLC 2017-12-20 /pmc/articles/PMC5788667/ /pubmed/29416799 http://dx.doi.org/10.18632/oncotarget.23474 Text en Copyright: © 2018 Duan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Duan, Jianchun
Yang, Xiaodan
Zhao, Jun
Zhuo, Minglei
Wang, Zhijie
An, Tongtong
Bai, Hua
Wang, Jie
Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title_full Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title_fullStr Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title_full_unstemmed Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title_short Correlation among genetic variations of c-MET in Chinese patients with non-small cell lung cancer
title_sort correlation among genetic variations of c-met in chinese patients with non-small cell lung cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788667/
https://www.ncbi.nlm.nih.gov/pubmed/29416799
http://dx.doi.org/10.18632/oncotarget.23474
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