Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection

The cell surface antigen prominin‐1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been gen...

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Autores principales: Karbanová, Jana, Lorico, Aurelio, Bornhäuser, Martin, Corbeil, Denis, Fargeas, Christine A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Perspectives
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788878/
https://www.ncbi.nlm.nih.gov/pubmed/29271118
http://dx.doi.org/10.1002/sctm.17-0223
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author Karbanová, Jana
Lorico, Aurelio
Bornhäuser, Martin
Corbeil, Denis
Fargeas, Christine A.
author_facet Karbanová, Jana
Lorico, Aurelio
Bornhäuser, Martin
Corbeil, Denis
Fargeas, Christine A.
author_sort Karbanová, Jana
collection PubMed
description The cell surface antigen prominin‐1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been generated in other systems given its more general expression beyond primitive cells. This article briefly discusses the importance of particular biochemical features of CD133 with relation to its association with membrane microdomains (lipid rafts) and proper immunodetection. It also draws attention toward the adequate use of detergents and caveats that may apply to the interpretation of the results generated. Stem Cells Translational Medicine 2018;7:155–160
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spelling pubmed-57888782018-02-08 Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection Karbanová, Jana Lorico, Aurelio Bornhäuser, Martin Corbeil, Denis Fargeas, Christine A. Stem Cells Transl Med Perspectives The cell surface antigen prominin‐1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been generated in other systems given its more general expression beyond primitive cells. This article briefly discusses the importance of particular biochemical features of CD133 with relation to its association with membrane microdomains (lipid rafts) and proper immunodetection. It also draws attention toward the adequate use of detergents and caveats that may apply to the interpretation of the results generated. Stem Cells Translational Medicine 2018;7:155–160 John Wiley and Sons Inc. 2017-12-22 /pmc/articles/PMC5788878/ /pubmed/29271118 http://dx.doi.org/10.1002/sctm.17-0223 Text en © 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Perspectives
Karbanová, Jana
Lorico, Aurelio
Bornhäuser, Martin
Corbeil, Denis
Fargeas, Christine A.
Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title_full Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title_fullStr Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title_full_unstemmed Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title_short Prominin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
title_sort prominin‐1/cd133: lipid raft association, detergent resistance, and immunodetection
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788878/
https://www.ncbi.nlm.nih.gov/pubmed/29271118
http://dx.doi.org/10.1002/sctm.17-0223
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