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Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation
Clinical application of induced pluripotent stem cells (iPS) in autologous settings has just begun. To overcome the high time and cost barriers in the individual production of autologous iPS, the use of allogeneic iPS with a homozygous human leukocyte antigen (HLA) haplotype (HLA‐homo HP) has been p...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788882/ https://www.ncbi.nlm.nih.gov/pubmed/29274116 http://dx.doi.org/10.1002/sctm.17-0169 |
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author | Morishima, Yasuo Azuma, Fumihiro Kashiwase, Koichi Matsumoto, Kayoko Orihara, Takeshi Yabe, Hiromasa Kato, Shunichi Kato, Koji Kai, Shunro Mori, Tetsuo Nakajima, Kazunori Morishima, Satoko Satake, Masahiro Takanashi, Minoko Yabe, Toshio |
author_facet | Morishima, Yasuo Azuma, Fumihiro Kashiwase, Koichi Matsumoto, Kayoko Orihara, Takeshi Yabe, Hiromasa Kato, Shunichi Kato, Koji Kai, Shunro Mori, Tetsuo Nakajima, Kazunori Morishima, Satoko Satake, Masahiro Takanashi, Minoko Yabe, Toshio |
author_sort | Morishima, Yasuo |
collection | PubMed |
description | Clinical application of induced pluripotent stem cells (iPS) in autologous settings has just begun. To overcome the high time and cost barriers in the individual production of autologous iPS, the use of allogeneic iPS with a homozygous human leukocyte antigen (HLA) haplotype (HLA‐homo HP) has been proposed. Cord blood transplantation (CBT) is a suitable model for evaluating the allogeneic immunogenicity of iPS transplantation from HLA‐homo donors. We analyzed 1,374 Japanese single cord blood transplant pairs who were retrospectively typed as HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1, and ‐DPB1. Among these, six pairs with donor HLA homo—patient‐HLA hetero (homo‐hetero) were found, all of which showed favorable neutrophil engraftment. Multivariate analysis revealed a significantly elevated engraftment risk (HR = 1.59) compared with hetero‐hetero pairs with HLA 1‐2 locus mismatch (789 pts) and comparative risk (HR = 1.23) compared with hetero‐hetero pairs with 0 mismatch (104 pts). These results for CBT with HLA‐homo HP cord blood carry an important implication, namely the possibility that HLA‐homo iPS transplantation results in favorable engraftment. Furthermore, we obtained detailed information on HLA alleles and haplotypes of HLA‐homo. All donor HLA‐homo HPs had a common specific ethnicity and high conservation of the HLA region, and one of two patient heterogeneous HPs invariably shared the same HP as donor HLA‐homo HP, and another non‐shared patient HP was mismatched with 1 to 4 HLA alleles of HLA‐A, ‐B, ‐C, and ‐DRB1 loci in the GVH direction. These findings indicate that patients possessing a single common HLA haplotype have a higher chance of yielding HLA‐homo iPS. Stem Cells Translational Medicine 2018;7:173–179 |
format | Online Article Text |
id | pubmed-5788882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57888822018-02-08 Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation Morishima, Yasuo Azuma, Fumihiro Kashiwase, Koichi Matsumoto, Kayoko Orihara, Takeshi Yabe, Hiromasa Kato, Shunichi Kato, Koji Kai, Shunro Mori, Tetsuo Nakajima, Kazunori Morishima, Satoko Satake, Masahiro Takanashi, Minoko Yabe, Toshio Stem Cells Transl Med Translational Research Articles and Reviews Clinical application of induced pluripotent stem cells (iPS) in autologous settings has just begun. To overcome the high time and cost barriers in the individual production of autologous iPS, the use of allogeneic iPS with a homozygous human leukocyte antigen (HLA) haplotype (HLA‐homo HP) has been proposed. Cord blood transplantation (CBT) is a suitable model for evaluating the allogeneic immunogenicity of iPS transplantation from HLA‐homo donors. We analyzed 1,374 Japanese single cord blood transplant pairs who were retrospectively typed as HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1, and ‐DPB1. Among these, six pairs with donor HLA homo—patient‐HLA hetero (homo‐hetero) were found, all of which showed favorable neutrophil engraftment. Multivariate analysis revealed a significantly elevated engraftment risk (HR = 1.59) compared with hetero‐hetero pairs with HLA 1‐2 locus mismatch (789 pts) and comparative risk (HR = 1.23) compared with hetero‐hetero pairs with 0 mismatch (104 pts). These results for CBT with HLA‐homo HP cord blood carry an important implication, namely the possibility that HLA‐homo iPS transplantation results in favorable engraftment. Furthermore, we obtained detailed information on HLA alleles and haplotypes of HLA‐homo. All donor HLA‐homo HPs had a common specific ethnicity and high conservation of the HLA region, and one of two patient heterogeneous HPs invariably shared the same HP as donor HLA‐homo HP, and another non‐shared patient HP was mismatched with 1 to 4 HLA alleles of HLA‐A, ‐B, ‐C, and ‐DRB1 loci in the GVH direction. These findings indicate that patients possessing a single common HLA haplotype have a higher chance of yielding HLA‐homo iPS. Stem Cells Translational Medicine 2018;7:173–179 John Wiley and Sons Inc. 2017-12-23 /pmc/articles/PMC5788882/ /pubmed/29274116 http://dx.doi.org/10.1002/sctm.17-0169 Text en © 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Translational Research Articles and Reviews Morishima, Yasuo Azuma, Fumihiro Kashiwase, Koichi Matsumoto, Kayoko Orihara, Takeshi Yabe, Hiromasa Kato, Shunichi Kato, Koji Kai, Shunro Mori, Tetsuo Nakajima, Kazunori Morishima, Satoko Satake, Masahiro Takanashi, Minoko Yabe, Toshio Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title | Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title_full | Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title_fullStr | Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title_full_unstemmed | Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title_short | Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation |
title_sort | risk of hla homozygous cord blood transplantation: implications for induced pluripotent stem cell banking and transplantation |
topic | Translational Research Articles and Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788882/ https://www.ncbi.nlm.nih.gov/pubmed/29274116 http://dx.doi.org/10.1002/sctm.17-0169 |
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