Cargando…

Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia

Diabetic peripheral neuropathy results in diabetic neuropathic pain (DNP). Satellite glial cells (SGCs) enwrap the neuronal soma in the dorsal root ganglia (DRG). The purinergic 2 (P2) Y12 receptor is expressed on SGCs in the DRG. SGC activation plays an important role in the pathogenesis of DNP. Cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Jia, Tianyu, Rao, Jingan, Zou, Lifang, Zhao, Shanhong, Yi, Zhihua, Wu, Bing, Li, Lin, Yuan, Huilong, Shi, Liran, Zhang, Chunping, Gao, Yun, Liu, Shuangmei, Xu, Hong, Liu, Hui, Liang, Shangdong, Li, Guilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788895/
https://www.ncbi.nlm.nih.gov/pubmed/29422835
http://dx.doi.org/10.3389/fnins.2017.00755
_version_ 1783296156184346624
author Jia, Tianyu
Rao, Jingan
Zou, Lifang
Zhao, Shanhong
Yi, Zhihua
Wu, Bing
Li, Lin
Yuan, Huilong
Shi, Liran
Zhang, Chunping
Gao, Yun
Liu, Shuangmei
Xu, Hong
Liu, Hui
Liang, Shangdong
Li, Guilin
author_facet Jia, Tianyu
Rao, Jingan
Zou, Lifang
Zhao, Shanhong
Yi, Zhihua
Wu, Bing
Li, Lin
Yuan, Huilong
Shi, Liran
Zhang, Chunping
Gao, Yun
Liu, Shuangmei
Xu, Hong
Liu, Hui
Liang, Shangdong
Li, Guilin
author_sort Jia, Tianyu
collection PubMed
description Diabetic peripheral neuropathy results in diabetic neuropathic pain (DNP). Satellite glial cells (SGCs) enwrap the neuronal soma in the dorsal root ganglia (DRG). The purinergic 2 (P2) Y12 receptor is expressed on SGCs in the DRG. SGC activation plays an important role in the pathogenesis of DNP. Curcumin has anti-inflammatory and antioxidant properties. Because curcumin has poor metabolic stability in vivo and low bioavailability, nanoparticle-encapsulated curcumin was used to improve its targeting and bioavailability. In the present study, our aim was to investigate the effects of nanoparticle-encapsulated curcumin on DNP mediated by the P2Y12 receptor on SGCs in the rat DRG. Diabetic peripheral neuropathy increased the expression levels of the P2Y12 receptor on SGCs in the DRG and enhanced mechanical and thermal hyperalgesia in rats with diabetes mellitus (DM). Up-regulation of the P2Y12 receptor in SGCs in the DRG increased the production of pro-inflammatory cytokines. Up-regulation of interleukin-1β (IL-1β) and connexin43 (Cx43) resulted in mechanical and thermal hyperalgesia in rats with DM. The nanoparticle-encapsulated curcumin decreased up-regulated IL-1β and Cx43 expression and reduced levels of phosphorylated-Akt (p-Akt) in the DRG of rats with DM. The up-regulation of P2Y12 on SGCs and the up-regulation of the IL-1β and Cx43 in the DRG indicated the activation of SGCs in the DRG. The nano-curcumin treatment inhibited the activation of SGCs accompanied by its anti-inflammatory effect to decrease the up-regulated CGRP expression in the DRG neurons. Therefore, the nanoparticle-encapsulated curcumin treatment decreased the up-regulation of the P2Y12 receptor on SGCs in the DRG and decreased mechanical and thermal hyperalgesia in rats with DM.
format Online
Article
Text
id pubmed-5788895
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-57888952018-02-08 Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia Jia, Tianyu Rao, Jingan Zou, Lifang Zhao, Shanhong Yi, Zhihua Wu, Bing Li, Lin Yuan, Huilong Shi, Liran Zhang, Chunping Gao, Yun Liu, Shuangmei Xu, Hong Liu, Hui Liang, Shangdong Li, Guilin Front Neurosci Neuroscience Diabetic peripheral neuropathy results in diabetic neuropathic pain (DNP). Satellite glial cells (SGCs) enwrap the neuronal soma in the dorsal root ganglia (DRG). The purinergic 2 (P2) Y12 receptor is expressed on SGCs in the DRG. SGC activation plays an important role in the pathogenesis of DNP. Curcumin has anti-inflammatory and antioxidant properties. Because curcumin has poor metabolic stability in vivo and low bioavailability, nanoparticle-encapsulated curcumin was used to improve its targeting and bioavailability. In the present study, our aim was to investigate the effects of nanoparticle-encapsulated curcumin on DNP mediated by the P2Y12 receptor on SGCs in the rat DRG. Diabetic peripheral neuropathy increased the expression levels of the P2Y12 receptor on SGCs in the DRG and enhanced mechanical and thermal hyperalgesia in rats with diabetes mellitus (DM). Up-regulation of the P2Y12 receptor in SGCs in the DRG increased the production of pro-inflammatory cytokines. Up-regulation of interleukin-1β (IL-1β) and connexin43 (Cx43) resulted in mechanical and thermal hyperalgesia in rats with DM. The nanoparticle-encapsulated curcumin decreased up-regulated IL-1β and Cx43 expression and reduced levels of phosphorylated-Akt (p-Akt) in the DRG of rats with DM. The up-regulation of P2Y12 on SGCs and the up-regulation of the IL-1β and Cx43 in the DRG indicated the activation of SGCs in the DRG. The nano-curcumin treatment inhibited the activation of SGCs accompanied by its anti-inflammatory effect to decrease the up-regulated CGRP expression in the DRG neurons. Therefore, the nanoparticle-encapsulated curcumin treatment decreased the up-regulation of the P2Y12 receptor on SGCs in the DRG and decreased mechanical and thermal hyperalgesia in rats with DM. Frontiers Media S.A. 2018-01-25 /pmc/articles/PMC5788895/ /pubmed/29422835 http://dx.doi.org/10.3389/fnins.2017.00755 Text en Copyright © 2018 Jia, Rao, Zou, Zhao, Yi, Wu, Li, Yuan, Shi, Zhang, Gao, Liu, Xu, Liu, Liang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Jia, Tianyu
Rao, Jingan
Zou, Lifang
Zhao, Shanhong
Yi, Zhihua
Wu, Bing
Li, Lin
Yuan, Huilong
Shi, Liran
Zhang, Chunping
Gao, Yun
Liu, Shuangmei
Xu, Hong
Liu, Hui
Liang, Shangdong
Li, Guilin
Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title_full Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title_fullStr Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title_full_unstemmed Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title_short Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia
title_sort nanoparticle-encapsulated curcumin inhibits diabetic neuropathic pain involving the p2y12 receptor in the dorsal root ganglia
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788895/
https://www.ncbi.nlm.nih.gov/pubmed/29422835
http://dx.doi.org/10.3389/fnins.2017.00755
work_keys_str_mv AT jiatianyu nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT raojingan nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT zoulifang nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT zhaoshanhong nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT yizhihua nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT wubing nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT lilin nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT yuanhuilong nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT shiliran nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT zhangchunping nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT gaoyun nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT liushuangmei nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT xuhong nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT liuhui nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT liangshangdong nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia
AT liguilin nanoparticleencapsulatedcurcumininhibitsdiabeticneuropathicpaininvolvingthep2y12receptorinthedorsalrootganglia