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Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015

Between 2014 and 2015, the Klebsiella pneumoniae carbapenemase (KPC) was becoming endemic in South Korea. To assess this period of transition, we analyzed KPC producers in terms of molecular epidemiology. A total of 362 KPC-producing Enterobacteriaceae strains, including one from 2013, 13 from 2014,...

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Autores principales: Yoon, Eun-Jeong, Kim, Jung Ok, Kim, Dokyun, Lee, Hyukmin, Yang, Ji Woo, Lee, Kwang Jun, Jeong, Seok Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788937/
https://www.ncbi.nlm.nih.gov/pubmed/29422888
http://dx.doi.org/10.3389/fmicb.2018.00056
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author Yoon, Eun-Jeong
Kim, Jung Ok
Kim, Dokyun
Lee, Hyukmin
Yang, Ji Woo
Lee, Kwang Jun
Jeong, Seok Hoon
author_facet Yoon, Eun-Jeong
Kim, Jung Ok
Kim, Dokyun
Lee, Hyukmin
Yang, Ji Woo
Lee, Kwang Jun
Jeong, Seok Hoon
author_sort Yoon, Eun-Jeong
collection PubMed
description Between 2014 and 2015, the Klebsiella pneumoniae carbapenemase (KPC) was becoming endemic in South Korea. To assess this period of transition, we analyzed KPC producers in terms of molecular epidemiology. A total of 362 KPC-producing Enterobacteriaceae strains, including one from 2013, 13 from 2014, and 348 from 2015, were actively collected from 60 hospitals throughout the peninsula. Subtypes of KPC were determined by PCR and direct sequencing, and isotypes of Tn4401 (the transposon flanking the bla(KPC) gene) were specified by PCR using isotype-specific primers and direct sequencing. Sporadic occurrence of KPC-producing Enterobacteriaceae was initially observed around Seoul, which is the most crowded district of the country, and these strains rapidly disseminated in 2014, to the other parts of the country in 2015. The bacterial clones responsible for the extreme epidemiological transition were K. pneumoniae ST307 (46.2%) and ST11 (21.3%). Less frequently, E. coli (4.7%), Enterobacter spp. (1.4%), and other Enterobacteriaceae members (1.7%) producing the enzyme were identified. The bla(KPC-2) gene bracketed by Tn4401a (72.1%) was the most prevalent mobile genetic element responsible for the dissemination, and the same gene carried either by Tn4401b (2.2%) or Tn4401c (6.6%) was identified at a lesser frequency. The genes bla(KPC-3) (1.6%) and bla(KPC-4) (6.4%), both flanked by Tn4401b, were occasionally identified. This study showed endemic dissemination of KPC producers in 2015 due to a clonal spread of two K. pneumoniae strains. Further systemic surveillance is needed to monitor dissemination of KPC-producers.
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spelling pubmed-57889372018-02-08 Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015 Yoon, Eun-Jeong Kim, Jung Ok Kim, Dokyun Lee, Hyukmin Yang, Ji Woo Lee, Kwang Jun Jeong, Seok Hoon Front Microbiol Microbiology Between 2014 and 2015, the Klebsiella pneumoniae carbapenemase (KPC) was becoming endemic in South Korea. To assess this period of transition, we analyzed KPC producers in terms of molecular epidemiology. A total of 362 KPC-producing Enterobacteriaceae strains, including one from 2013, 13 from 2014, and 348 from 2015, were actively collected from 60 hospitals throughout the peninsula. Subtypes of KPC were determined by PCR and direct sequencing, and isotypes of Tn4401 (the transposon flanking the bla(KPC) gene) were specified by PCR using isotype-specific primers and direct sequencing. Sporadic occurrence of KPC-producing Enterobacteriaceae was initially observed around Seoul, which is the most crowded district of the country, and these strains rapidly disseminated in 2014, to the other parts of the country in 2015. The bacterial clones responsible for the extreme epidemiological transition were K. pneumoniae ST307 (46.2%) and ST11 (21.3%). Less frequently, E. coli (4.7%), Enterobacter spp. (1.4%), and other Enterobacteriaceae members (1.7%) producing the enzyme were identified. The bla(KPC-2) gene bracketed by Tn4401a (72.1%) was the most prevalent mobile genetic element responsible for the dissemination, and the same gene carried either by Tn4401b (2.2%) or Tn4401c (6.6%) was identified at a lesser frequency. The genes bla(KPC-3) (1.6%) and bla(KPC-4) (6.4%), both flanked by Tn4401b, were occasionally identified. This study showed endemic dissemination of KPC producers in 2015 due to a clonal spread of two K. pneumoniae strains. Further systemic surveillance is needed to monitor dissemination of KPC-producers. Frontiers Media S.A. 2018-01-25 /pmc/articles/PMC5788937/ /pubmed/29422888 http://dx.doi.org/10.3389/fmicb.2018.00056 Text en Copyright © 2018 Yoon, Kim, Kim, Lee, Yang, Lee and Jeong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yoon, Eun-Jeong
Kim, Jung Ok
Kim, Dokyun
Lee, Hyukmin
Yang, Ji Woo
Lee, Kwang Jun
Jeong, Seok Hoon
Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title_full Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title_fullStr Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title_full_unstemmed Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title_short Klebsiella pneumoniae Carbapenemase Producers in South Korea between 2013 and 2015
title_sort klebsiella pneumoniae carbapenemase producers in south korea between 2013 and 2015
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788937/
https://www.ncbi.nlm.nih.gov/pubmed/29422888
http://dx.doi.org/10.3389/fmicb.2018.00056
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