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The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)

Aflatoxin B(1) shows potent hepatotoxic, carcinogenic, genotoxic, immunotoxic potential in humans and many species of animals. The aim of this study was to clarify the underlying mechanism of G(0)G(1) phase and G(2)M phase arrest of cell cycle in the bursa of Fabricius in broilers exposed to dietary...

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Autores principales: Hu, Ping, Zuo, Zhicai, Li, Hang, Wang, Fengyuan, Peng, Xi, Fang, Jing, Cui, Hengmin, Gao, Caixia, Song, Hetao, Zhou, Yi, Chen, Zhengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789014/
https://www.ncbi.nlm.nih.gov/pubmed/29379099
http://dx.doi.org/10.1038/s41598-018-20164-z
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author Hu, Ping
Zuo, Zhicai
Li, Hang
Wang, Fengyuan
Peng, Xi
Fang, Jing
Cui, Hengmin
Gao, Caixia
Song, Hetao
Zhou, Yi
Chen, Zhengli
author_facet Hu, Ping
Zuo, Zhicai
Li, Hang
Wang, Fengyuan
Peng, Xi
Fang, Jing
Cui, Hengmin
Gao, Caixia
Song, Hetao
Zhou, Yi
Chen, Zhengli
author_sort Hu, Ping
collection PubMed
description Aflatoxin B(1) shows potent hepatotoxic, carcinogenic, genotoxic, immunotoxic potential in humans and many species of animals. The aim of this study was to clarify the underlying mechanism of G(0)G(1) phase and G(2)M phase arrest of cell cycle in the bursa of Fabricius in broilers exposed to dietary AFB(1). 144 one-day-old healthy Cobb broilers were randomly divided into two groups and fed on control diet and 0.6 mg·Kg(−1) AFB(1) diet for 3 weeks. Histological observation showed that AFB(1) induced the increase of nuclear debris and vacuoles in lymphoid follicle of BF. Results of flow cytometry studies showed that bursal cells arrested in G(2)M phase at 7 days of age and blocked in G(0)G(1) phase at 14 and 21 days of age following exposure to AFB(1). The qRT-PCR analysis indicated that cell cycle arrested in G(2)M phase via ATM-Chk2-cdc25-cyclin B/cdc2 pathway, and blocked in G(0)G(1) phase through ATM-Chk2-cdc25-cyclin D/CDK6 pathway and ATM-Chk2-p21-cyclin D/CDK6 route. In a word, our results provided new insights that AFB(1) diet induced G(2)M and G(0)G(1) phase blockage of BF cells in different periods, and different pathways were activated in different arrested cell cycle phase.
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spelling pubmed-57890142018-02-08 The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1) Hu, Ping Zuo, Zhicai Li, Hang Wang, Fengyuan Peng, Xi Fang, Jing Cui, Hengmin Gao, Caixia Song, Hetao Zhou, Yi Chen, Zhengli Sci Rep Article Aflatoxin B(1) shows potent hepatotoxic, carcinogenic, genotoxic, immunotoxic potential in humans and many species of animals. The aim of this study was to clarify the underlying mechanism of G(0)G(1) phase and G(2)M phase arrest of cell cycle in the bursa of Fabricius in broilers exposed to dietary AFB(1). 144 one-day-old healthy Cobb broilers were randomly divided into two groups and fed on control diet and 0.6 mg·Kg(−1) AFB(1) diet for 3 weeks. Histological observation showed that AFB(1) induced the increase of nuclear debris and vacuoles in lymphoid follicle of BF. Results of flow cytometry studies showed that bursal cells arrested in G(2)M phase at 7 days of age and blocked in G(0)G(1) phase at 14 and 21 days of age following exposure to AFB(1). The qRT-PCR analysis indicated that cell cycle arrested in G(2)M phase via ATM-Chk2-cdc25-cyclin B/cdc2 pathway, and blocked in G(0)G(1) phase through ATM-Chk2-cdc25-cyclin D/CDK6 pathway and ATM-Chk2-p21-cyclin D/CDK6 route. In a word, our results provided new insights that AFB(1) diet induced G(2)M and G(0)G(1) phase blockage of BF cells in different periods, and different pathways were activated in different arrested cell cycle phase. Nature Publishing Group UK 2018-01-29 /pmc/articles/PMC5789014/ /pubmed/29379099 http://dx.doi.org/10.1038/s41598-018-20164-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hu, Ping
Zuo, Zhicai
Li, Hang
Wang, Fengyuan
Peng, Xi
Fang, Jing
Cui, Hengmin
Gao, Caixia
Song, Hetao
Zhou, Yi
Chen, Zhengli
The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title_full The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title_fullStr The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title_full_unstemmed The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title_short The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B(1)
title_sort molecular mechanism of cell cycle arrest in the bursa of fabricius in chick exposed to aflatoxin b(1)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789014/
https://www.ncbi.nlm.nih.gov/pubmed/29379099
http://dx.doi.org/10.1038/s41598-018-20164-z
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