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Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo

We generated induced pluripotent stem cells (iPSCs) from patient fibroblasts to yield cell lines containing varying degrees of heteroplasmy for a m.13514 A > G mtDNA point mutation (2 lines) and for a ~6 kb single, large scale mtDNA deletion (3 lines). Long term culture of the iPSCs containing a ...

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Detalles Bibliográficos
Autores principales: Russell, Oliver M., Fruh, Isabelle, Rai, Pavandeep K., Marcellin, David, Doll, Thierry, Reeve, Amy, Germain, Mitchel, Bastien, Julie, Rygiel, Karolina A., Cerino, Raffaele, Sailer, Andreas W., Lako, Majlinda, Taylor, Robert W., Mueller, Matthias, Lightowlers, Robert N., Turnbull, Doug M., Helliwell, Stephen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789095/
https://www.ncbi.nlm.nih.gov/pubmed/29379065
http://dx.doi.org/10.1038/s41598-018-20064-2
Descripción
Sumario:We generated induced pluripotent stem cells (iPSCs) from patient fibroblasts to yield cell lines containing varying degrees of heteroplasmy for a m.13514 A > G mtDNA point mutation (2 lines) and for a ~6 kb single, large scale mtDNA deletion (3 lines). Long term culture of the iPSCs containing a single, large-scale mtDNA deletion showed consistent increase in mtDNA deletion levels with time. Higher levels of mtDNA heteroplasmy correlated with increased respiratory deficiency. To determine what changes occurred in deletion level during differentiation, teratomas comprising all three embryonic germ layers were generated from low (20%) and intermediate heteroplasmy (55%) mtDNA deletion clones. Regardless of whether iPSCs harbouring low or intermediate mtDNA heteroplasmy were used, the final levels of heteroplasmy in all teratoma germ layers increased to a similar high level (>60%). Thus, during human stem cell division, cells not only tolerate high mtDNA deletion loads but seem to preferentially replicate deleted mtDNA genomes. This has implications for the involvement of mtDNA deletions in both disease and ageing.