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Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury
Traumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor functio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789342/ https://www.ncbi.nlm.nih.gov/pubmed/29316623 http://dx.doi.org/10.3390/brainsci8010011 |
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author | Bhowmick, Saurav D‘Mello, Veera Ponery, Nizmi Abdul-Muneer, P. M. |
author_facet | Bhowmick, Saurav D‘Mello, Veera Ponery, Nizmi Abdul-Muneer, P. M. |
author_sort | Bhowmick, Saurav |
collection | PubMed |
description | Traumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor function. TBI induces the activation of the caspase-3 enzyme, which triggers cell apoptosis in an in vivo model of fluid percussion injury (FPI). We analyzed caspase-3 mediated apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and poly (ADP-ribose) polymerase (PARP) and annexin V western blotting. We correlated the neurodegeneration with sensorimotor deficits by conducting the animal behavioral tests including grid walk, balance beam, the inverted screen test, and the climb test. Our study demonstrated that the excess cell death or neurodegeneration correlated with the neuronal dysfunction and sensorimotor impairments associated with TBI. |
format | Online Article Text |
id | pubmed-5789342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57893422018-02-02 Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury Bhowmick, Saurav D‘Mello, Veera Ponery, Nizmi Abdul-Muneer, P. M. Brain Sci Article Traumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor function. TBI induces the activation of the caspase-3 enzyme, which triggers cell apoptosis in an in vivo model of fluid percussion injury (FPI). We analyzed caspase-3 mediated apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and poly (ADP-ribose) polymerase (PARP) and annexin V western blotting. We correlated the neurodegeneration with sensorimotor deficits by conducting the animal behavioral tests including grid walk, balance beam, the inverted screen test, and the climb test. Our study demonstrated that the excess cell death or neurodegeneration correlated with the neuronal dysfunction and sensorimotor impairments associated with TBI. MDPI 2018-01-06 /pmc/articles/PMC5789342/ /pubmed/29316623 http://dx.doi.org/10.3390/brainsci8010011 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bhowmick, Saurav D‘Mello, Veera Ponery, Nizmi Abdul-Muneer, P. M. Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title | Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_full | Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_fullStr | Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_full_unstemmed | Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_short | Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_sort | neurodegeneration and sensorimotor deficits in the mouse model of traumatic brain injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789342/ https://www.ncbi.nlm.nih.gov/pubmed/29316623 http://dx.doi.org/10.3390/brainsci8010011 |
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