Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains

BACKGROUND: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol comb...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yi-ming, Kong, Ling-cong, Liu, Jie, Ma, Hong-xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789579/
https://www.ncbi.nlm.nih.gov/pubmed/29423192
http://dx.doi.org/10.1186/s13756-018-0303-7
_version_ 1783296307678412800
author Wang, Yi-ming
Kong, Ling-cong
Liu, Jie
Ma, Hong-xia
author_facet Wang, Yi-ming
Kong, Ling-cong
Liu, Jie
Ma, Hong-xia
author_sort Wang, Yi-ming
collection PubMed
description BACKGROUND: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol combined with colistin against a collection of clinical isolated Escherichia coli (E.coli) strains, and to evaluate potential interaction. METHODS: Antimicrobial susceptibility, minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) of the bacteria were determined by disk diffusion assay, broth microdilution method and checkerboard assay, respectively. The mcr-1 mRNA expression was measured by Real-time PCR. To predict possible interactions between eugenol and MCR-1, molecular docking assay was taken. RESULTS: For total fourteen strains including eight colistin-resistant strains, eugenol was determined with MIC values of 4 to 8 μg/mL. Checkerboard dilution test suggested that eugenol exhibited synergistic activity when combined with colistin (FICI ranging from 0.375 to 0.625). Comparison analysis of Real-time PCR showed that synergy could significantly down-regulate expression of mcr-1 gene. A metal ion coordination bond with catalytic zinc atom and a hydrogen bond with crucial amino acid residue Ser284 of MCR-1 were observed after molecular docking, indicating antibacterial activity and direct molecular interactions of eugenol with MCR-1 protein. CONCLUSIONS: Our results demonstrated that eugenol exhibited synergistic effect with colistin and enhanced its antimicrobial activity. This might further contribute to the antibacterial actions against colistin-resistant E.coli strains. GRAPHICAL ABSTRACT: Synergistic effect of eugenol with colistin against colistin-resistant Escherichia coli isolates. [Image: see text]
format Online
Article
Text
id pubmed-5789579
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-57895792018-02-08 Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains Wang, Yi-ming Kong, Ling-cong Liu, Jie Ma, Hong-xia Antimicrob Resist Infect Control Research BACKGROUND: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol combined with colistin against a collection of clinical isolated Escherichia coli (E.coli) strains, and to evaluate potential interaction. METHODS: Antimicrobial susceptibility, minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) of the bacteria were determined by disk diffusion assay, broth microdilution method and checkerboard assay, respectively. The mcr-1 mRNA expression was measured by Real-time PCR. To predict possible interactions between eugenol and MCR-1, molecular docking assay was taken. RESULTS: For total fourteen strains including eight colistin-resistant strains, eugenol was determined with MIC values of 4 to 8 μg/mL. Checkerboard dilution test suggested that eugenol exhibited synergistic activity when combined with colistin (FICI ranging from 0.375 to 0.625). Comparison analysis of Real-time PCR showed that synergy could significantly down-regulate expression of mcr-1 gene. A metal ion coordination bond with catalytic zinc atom and a hydrogen bond with crucial amino acid residue Ser284 of MCR-1 were observed after molecular docking, indicating antibacterial activity and direct molecular interactions of eugenol with MCR-1 protein. CONCLUSIONS: Our results demonstrated that eugenol exhibited synergistic effect with colistin and enhanced its antimicrobial activity. This might further contribute to the antibacterial actions against colistin-resistant E.coli strains. GRAPHICAL ABSTRACT: Synergistic effect of eugenol with colistin against colistin-resistant Escherichia coli isolates. [Image: see text] BioMed Central 2018-01-29 /pmc/articles/PMC5789579/ /pubmed/29423192 http://dx.doi.org/10.1186/s13756-018-0303-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Yi-ming
Kong, Ling-cong
Liu, Jie
Ma, Hong-xia
Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title_full Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title_fullStr Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title_full_unstemmed Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title_short Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant Escherichia coli strains
title_sort synergistic effect of eugenol with colistin against clinical isolated colistin-resistant escherichia coli strains
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789579/
https://www.ncbi.nlm.nih.gov/pubmed/29423192
http://dx.doi.org/10.1186/s13756-018-0303-7
work_keys_str_mv AT wangyiming synergisticeffectofeugenolwithcolistinagainstclinicalisolatedcolistinresistantescherichiacolistrains
AT konglingcong synergisticeffectofeugenolwithcolistinagainstclinicalisolatedcolistinresistantescherichiacolistrains
AT liujie synergisticeffectofeugenolwithcolistinagainstclinicalisolatedcolistinresistantescherichiacolistrains
AT mahongxia synergisticeffectofeugenolwithcolistinagainstclinicalisolatedcolistinresistantescherichiacolistrains