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Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas

Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effective...

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Autores principales: Dolgova, Evgeniya V., Mishinov, Sergey V., Proskurina, Anastasiya S., Potter, Ekaterina A., Efremov, Yaroslav R., Bayborodin, Sergey I., Tyrinova, Tamara V., Stupak, Vyacheslav V., Ostatin, Alexandr A., Chernykh, Elena R., Bogachev, Sergey S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789816/
https://www.ncbi.nlm.nih.gov/pubmed/29375020
http://dx.doi.org/10.1177/1533034617753812
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author Dolgova, Evgeniya V.
Mishinov, Sergey V.
Proskurina, Anastasiya S.
Potter, Ekaterina A.
Efremov, Yaroslav R.
Bayborodin, Sergey I.
Tyrinova, Tamara V.
Stupak, Vyacheslav V.
Ostatin, Alexandr A.
Chernykh, Elena R.
Bogachev, Sergey S.
author_facet Dolgova, Evgeniya V.
Mishinov, Sergey V.
Proskurina, Anastasiya S.
Potter, Ekaterina A.
Efremov, Yaroslav R.
Bayborodin, Sergey I.
Tyrinova, Tamara V.
Stupak, Vyacheslav V.
Ostatin, Alexandr A.
Chernykh, Elena R.
Bogachev, Sergey S.
author_sort Dolgova, Evgeniya V.
collection PubMed
description Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effectively internalize a fluorescently labeled DNA probe. Of these, using samples from 3 patients, 66% to 100% cells are also positive for CD133, a well-established surface marker of tumor-initiating glioma stem cells. Using the samples from primary malignant brain lesions (33 patients), we demonstrate that tumor grading significantly correlates (R = .71) with the percentage of DNA-internalizing cells. No such correlation is observed for relapse samples (18 patients).
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spelling pubmed-57898162018-02-02 Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas Dolgova, Evgeniya V. Mishinov, Sergey V. Proskurina, Anastasiya S. Potter, Ekaterina A. Efremov, Yaroslav R. Bayborodin, Sergey I. Tyrinova, Tamara V. Stupak, Vyacheslav V. Ostatin, Alexandr A. Chernykh, Elena R. Bogachev, Sergey S. Technol Cancer Res Treat Original Article Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effectively internalize a fluorescently labeled DNA probe. Of these, using samples from 3 patients, 66% to 100% cells are also positive for CD133, a well-established surface marker of tumor-initiating glioma stem cells. Using the samples from primary malignant brain lesions (33 patients), we demonstrate that tumor grading significantly correlates (R = .71) with the percentage of DNA-internalizing cells. No such correlation is observed for relapse samples (18 patients). SAGE Publications 2018-01-28 /pmc/articles/PMC5789816/ /pubmed/29375020 http://dx.doi.org/10.1177/1533034617753812 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Dolgova, Evgeniya V.
Mishinov, Sergey V.
Proskurina, Anastasiya S.
Potter, Ekaterina A.
Efremov, Yaroslav R.
Bayborodin, Sergey I.
Tyrinova, Tamara V.
Stupak, Vyacheslav V.
Ostatin, Alexandr A.
Chernykh, Elena R.
Bogachev, Sergey S.
Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title_full Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title_fullStr Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title_full_unstemmed Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title_short Novel Cancer Stem Marker and Its Applicability for Grading Primary Human Gliomas
title_sort novel cancer stem marker and its applicability for grading primary human gliomas
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789816/
https://www.ncbi.nlm.nih.gov/pubmed/29375020
http://dx.doi.org/10.1177/1533034617753812
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