Cargando…
Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis
Vgll3 is linked to age at maturity in Atlantic salmon (Salmo salar). However, the molecular mechanisms involving Vgll3 in controlling timing of puberty as well as relevant tissue and cell types are currently unknown. Vgll3 and the associated Hippo pathway has been linked to reduced proliferation act...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789820/ https://www.ncbi.nlm.nih.gov/pubmed/29382956 http://dx.doi.org/10.1038/s41598-018-20308-1 |
_version_ | 1783296355005890560 |
---|---|
author | Kjærner-Semb, Erik Ayllon, Fernando Kleppe, Lene Sørhus, Elin Skaftnesmo, Kai Furmanek, Tomasz Segafredo, Frida T. Thorsen, Anders Fjelldal, Per Gunnar Hansen, Tom Taranger, Geir Lasse Andersson, Eva Schulz, Rüdiger W. Wargelius, Anna Edvardsen, Rolf B. |
author_facet | Kjærner-Semb, Erik Ayllon, Fernando Kleppe, Lene Sørhus, Elin Skaftnesmo, Kai Furmanek, Tomasz Segafredo, Frida T. Thorsen, Anders Fjelldal, Per Gunnar Hansen, Tom Taranger, Geir Lasse Andersson, Eva Schulz, Rüdiger W. Wargelius, Anna Edvardsen, Rolf B. |
author_sort | Kjærner-Semb, Erik |
collection | PubMed |
description | Vgll3 is linked to age at maturity in Atlantic salmon (Salmo salar). However, the molecular mechanisms involving Vgll3 in controlling timing of puberty as well as relevant tissue and cell types are currently unknown. Vgll3 and the associated Hippo pathway has been linked to reduced proliferation activity in different tissues. Analysis of gene expression reveals for the first time that vgll3 and several members of the Hippo pathway were down-regulated in salmon testis during onset of puberty and remained repressed in maturing testis. In the gonads, we found expression in Sertoli and granulosa cells in males and females, respectively. We hypothesize that vgll3 negatively regulates Sertoli cell proliferation in testis and therefore acts as an inhibitor of pubertal testis growth. Gonadal expression of vgll3 is located to somatic cells that are in direct contact with germ cells in both sexes, however our results indicate sex-biased regulation of vgll3 during puberty. |
format | Online Article Text |
id | pubmed-5789820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57898202018-02-15 Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis Kjærner-Semb, Erik Ayllon, Fernando Kleppe, Lene Sørhus, Elin Skaftnesmo, Kai Furmanek, Tomasz Segafredo, Frida T. Thorsen, Anders Fjelldal, Per Gunnar Hansen, Tom Taranger, Geir Lasse Andersson, Eva Schulz, Rüdiger W. Wargelius, Anna Edvardsen, Rolf B. Sci Rep Article Vgll3 is linked to age at maturity in Atlantic salmon (Salmo salar). However, the molecular mechanisms involving Vgll3 in controlling timing of puberty as well as relevant tissue and cell types are currently unknown. Vgll3 and the associated Hippo pathway has been linked to reduced proliferation activity in different tissues. Analysis of gene expression reveals for the first time that vgll3 and several members of the Hippo pathway were down-regulated in salmon testis during onset of puberty and remained repressed in maturing testis. In the gonads, we found expression in Sertoli and granulosa cells in males and females, respectively. We hypothesize that vgll3 negatively regulates Sertoli cell proliferation in testis and therefore acts as an inhibitor of pubertal testis growth. Gonadal expression of vgll3 is located to somatic cells that are in direct contact with germ cells in both sexes, however our results indicate sex-biased regulation of vgll3 during puberty. Nature Publishing Group UK 2018-01-30 /pmc/articles/PMC5789820/ /pubmed/29382956 http://dx.doi.org/10.1038/s41598-018-20308-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kjærner-Semb, Erik Ayllon, Fernando Kleppe, Lene Sørhus, Elin Skaftnesmo, Kai Furmanek, Tomasz Segafredo, Frida T. Thorsen, Anders Fjelldal, Per Gunnar Hansen, Tom Taranger, Geir Lasse Andersson, Eva Schulz, Rüdiger W. Wargelius, Anna Edvardsen, Rolf B. Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title | Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title_full | Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title_fullStr | Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title_full_unstemmed | Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title_short | Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis |
title_sort | vgll3 and the hippo pathway are regulated in sertoli cells upon entry and during puberty in atlantic salmon testis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789820/ https://www.ncbi.nlm.nih.gov/pubmed/29382956 http://dx.doi.org/10.1038/s41598-018-20308-1 |
work_keys_str_mv | AT kjærnersemberik vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT ayllonfernando vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT kleppelene vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT sørhuselin vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT skaftnesmokai vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT furmanektomasz vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT segafredofridat vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT thorsenanders vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT fjelldalpergunnar vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT hansentom vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT tarangergeirlasse vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT anderssoneva vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT schulzrudigerw vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT wargeliusanna vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis AT edvardsenrolfb vgll3andthehippopathwayareregulatedinsertolicellsuponentryandduringpubertyinatlanticsalmontestis |