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Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45

Rice brown spot (BS), caused by Bipolaris oryzae, is one of the major diseases of rice in Japan. Quantitative resistance has been observed in local cultivars (e.g., CH45), but no economically useful resistant variety has been bred. Using simple sequence repeat (SSR) polymorphic markers, we conducted...

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Autores principales: Matsumoto, Kengo, Ota, Yuya, Seta, Satomi, Nakayama, Yukinori, Ohno, Teppei, Mizobuchi, Ritsuko, Sato, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790048/
https://www.ncbi.nlm.nih.gov/pubmed/29398949
http://dx.doi.org/10.1270/jsbbs.17057
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author Matsumoto, Kengo
Ota, Yuya
Seta, Satomi
Nakayama, Yukinori
Ohno, Teppei
Mizobuchi, Ritsuko
Sato, Hiroyuki
author_facet Matsumoto, Kengo
Ota, Yuya
Seta, Satomi
Nakayama, Yukinori
Ohno, Teppei
Mizobuchi, Ritsuko
Sato, Hiroyuki
author_sort Matsumoto, Kengo
collection PubMed
description Rice brown spot (BS), caused by Bipolaris oryzae, is one of the major diseases of rice in Japan. Quantitative resistance has been observed in local cultivars (e.g., CH45), but no economically useful resistant variety has been bred. Using simple sequence repeat (SSR) polymorphic markers, we conducted quantitative trait locus (QTL) analysis of BS resistance in backcross inbred lines (BILs) from a cross between indica CH45 (resistant) and japonica Koshihikari (susceptible). On the basis of field disease evaluations in 2015 and 2016, four QTLs contributing to BS resistance were identified on chromosomes 2 (qBSR2-kc), 7 (qBSR7-kc), 9 (qBSR9-kc), and 11 (qBSR11-kc). The ‘CH45’ alleles at qBSR2-kc, qBSR7-kc, and qBSR11-kc and the ‘Koshihikari’ allele at qBSR9-kc increased resistance. The major QTL qBSR11-kc explained 23.0%–25.9% of the total phenotypic variation. Two QTLs (qBSR9-kc and qBSR11-kc) were detected in both years, whereas the other two were detected only in 2016. Genetic markers flanking these four QTLs will be powerful tools for marker-assisted selection to improve BS resistance.
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spelling pubmed-57900482018-02-02 Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45 Matsumoto, Kengo Ota, Yuya Seta, Satomi Nakayama, Yukinori Ohno, Teppei Mizobuchi, Ritsuko Sato, Hiroyuki Breed Sci Note Rice brown spot (BS), caused by Bipolaris oryzae, is one of the major diseases of rice in Japan. Quantitative resistance has been observed in local cultivars (e.g., CH45), but no economically useful resistant variety has been bred. Using simple sequence repeat (SSR) polymorphic markers, we conducted quantitative trait locus (QTL) analysis of BS resistance in backcross inbred lines (BILs) from a cross between indica CH45 (resistant) and japonica Koshihikari (susceptible). On the basis of field disease evaluations in 2015 and 2016, four QTLs contributing to BS resistance were identified on chromosomes 2 (qBSR2-kc), 7 (qBSR7-kc), 9 (qBSR9-kc), and 11 (qBSR11-kc). The ‘CH45’ alleles at qBSR2-kc, qBSR7-kc, and qBSR11-kc and the ‘Koshihikari’ allele at qBSR9-kc increased resistance. The major QTL qBSR11-kc explained 23.0%–25.9% of the total phenotypic variation. Two QTLs (qBSR9-kc and qBSR11-kc) were detected in both years, whereas the other two were detected only in 2016. Genetic markers flanking these four QTLs will be powerful tools for marker-assisted selection to improve BS resistance. Japanese Society of Breeding 2017-12 2017-11-16 /pmc/articles/PMC5790048/ /pubmed/29398949 http://dx.doi.org/10.1270/jsbbs.17057 Text en Copyright © 2017 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Matsumoto, Kengo
Ota, Yuya
Seta, Satomi
Nakayama, Yukinori
Ohno, Teppei
Mizobuchi, Ritsuko
Sato, Hiroyuki
Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title_full Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title_fullStr Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title_full_unstemmed Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title_short Identification of QTLs for rice brown spot resistance in backcross inbred lines derived from a cross between Koshihikari and CH45
title_sort identification of qtls for rice brown spot resistance in backcross inbred lines derived from a cross between koshihikari and ch45
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790048/
https://www.ncbi.nlm.nih.gov/pubmed/29398949
http://dx.doi.org/10.1270/jsbbs.17057
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