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Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair

Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimenti...

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Autores principales: LeBert, Danny, Squirrell, Jayne M, Freisinger, Chrissy, Rindy, Julie, Golenberg, Netta, Frecentese, Grace, Gibson, Angela, Eliceiri, Kevin W, Huttenlocher, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790375/
https://www.ncbi.nlm.nih.gov/pubmed/29336778
http://dx.doi.org/10.7554/eLife.30703
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author LeBert, Danny
Squirrell, Jayne M
Freisinger, Chrissy
Rindy, Julie
Golenberg, Netta
Frecentese, Grace
Gibson, Angela
Eliceiri, Kevin W
Huttenlocher, Anna
author_facet LeBert, Danny
Squirrell, Jayne M
Freisinger, Chrissy
Rindy, Julie
Golenberg, Netta
Frecentese, Grace
Gibson, Angela
Eliceiri, Kevin W
Huttenlocher, Anna
author_sort LeBert, Danny
collection PubMed
description Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimentin promoter in zebrafish. Early redox signaling mediated vimentin reporter activity at the wound margin. Moreover, both ROS and vimentin were necessary for collagen production and reorganization into projections at the leading edge of the wound. Second harmonic generation time-lapse imaging revealed that the collagen projections were associated with dynamic epithelial extensions at the wound edge during wound repair. Perturbing collagen organization by burn wound disrupted epithelial projections and subsequent wound healing. Taken together our findings suggest that ROS and vimentin integrate early wound signals to orchestrate the formation of collagen-based projections that guide regenerative growth during efficient wound repair.
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spelling pubmed-57903752018-01-31 Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair LeBert, Danny Squirrell, Jayne M Freisinger, Chrissy Rindy, Julie Golenberg, Netta Frecentese, Grace Gibson, Angela Eliceiri, Kevin W Huttenlocher, Anna eLife Cell Biology Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimentin promoter in zebrafish. Early redox signaling mediated vimentin reporter activity at the wound margin. Moreover, both ROS and vimentin were necessary for collagen production and reorganization into projections at the leading edge of the wound. Second harmonic generation time-lapse imaging revealed that the collagen projections were associated with dynamic epithelial extensions at the wound edge during wound repair. Perturbing collagen organization by burn wound disrupted epithelial projections and subsequent wound healing. Taken together our findings suggest that ROS and vimentin integrate early wound signals to orchestrate the formation of collagen-based projections that guide regenerative growth during efficient wound repair. eLife Sciences Publications, Ltd 2018-01-16 /pmc/articles/PMC5790375/ /pubmed/29336778 http://dx.doi.org/10.7554/eLife.30703 Text en © 2018, LeBert et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
LeBert, Danny
Squirrell, Jayne M
Freisinger, Chrissy
Rindy, Julie
Golenberg, Netta
Frecentese, Grace
Gibson, Angela
Eliceiri, Kevin W
Huttenlocher, Anna
Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title_full Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title_fullStr Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title_full_unstemmed Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title_short Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
title_sort damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790375/
https://www.ncbi.nlm.nih.gov/pubmed/29336778
http://dx.doi.org/10.7554/eLife.30703
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