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Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair
Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimenti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790375/ https://www.ncbi.nlm.nih.gov/pubmed/29336778 http://dx.doi.org/10.7554/eLife.30703 |
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author | LeBert, Danny Squirrell, Jayne M Freisinger, Chrissy Rindy, Julie Golenberg, Netta Frecentese, Grace Gibson, Angela Eliceiri, Kevin W Huttenlocher, Anna |
author_facet | LeBert, Danny Squirrell, Jayne M Freisinger, Chrissy Rindy, Julie Golenberg, Netta Frecentese, Grace Gibson, Angela Eliceiri, Kevin W Huttenlocher, Anna |
author_sort | LeBert, Danny |
collection | PubMed |
description | Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimentin promoter in zebrafish. Early redox signaling mediated vimentin reporter activity at the wound margin. Moreover, both ROS and vimentin were necessary for collagen production and reorganization into projections at the leading edge of the wound. Second harmonic generation time-lapse imaging revealed that the collagen projections were associated with dynamic epithelial extensions at the wound edge during wound repair. Perturbing collagen organization by burn wound disrupted epithelial projections and subsequent wound healing. Taken together our findings suggest that ROS and vimentin integrate early wound signals to orchestrate the formation of collagen-based projections that guide regenerative growth during efficient wound repair. |
format | Online Article Text |
id | pubmed-5790375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57903752018-01-31 Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair LeBert, Danny Squirrell, Jayne M Freisinger, Chrissy Rindy, Julie Golenberg, Netta Frecentese, Grace Gibson, Angela Eliceiri, Kevin W Huttenlocher, Anna eLife Cell Biology Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimentin promoter in zebrafish. Early redox signaling mediated vimentin reporter activity at the wound margin. Moreover, both ROS and vimentin were necessary for collagen production and reorganization into projections at the leading edge of the wound. Second harmonic generation time-lapse imaging revealed that the collagen projections were associated with dynamic epithelial extensions at the wound edge during wound repair. Perturbing collagen organization by burn wound disrupted epithelial projections and subsequent wound healing. Taken together our findings suggest that ROS and vimentin integrate early wound signals to orchestrate the formation of collagen-based projections that guide regenerative growth during efficient wound repair. eLife Sciences Publications, Ltd 2018-01-16 /pmc/articles/PMC5790375/ /pubmed/29336778 http://dx.doi.org/10.7554/eLife.30703 Text en © 2018, LeBert et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology LeBert, Danny Squirrell, Jayne M Freisinger, Chrissy Rindy, Julie Golenberg, Netta Frecentese, Grace Gibson, Angela Eliceiri, Kevin W Huttenlocher, Anna Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title | Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title_full | Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title_fullStr | Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title_full_unstemmed | Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title_short | Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
title_sort | damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790375/ https://www.ncbi.nlm.nih.gov/pubmed/29336778 http://dx.doi.org/10.7554/eLife.30703 |
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