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Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway

Postinfarct cardiac hypertrophy is an independent risk factor for heart failure and sudden death. Regression of cardiac hypertrophy has emerged as a promising strategy in the treatment of myocardial infarction (MI). Here we hypothesized that frizzled1 (FZD1), a receptor of the canonical Wnt signalin...

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Autores principales: Fan, Jingjing, Qiu, Lin, Shu, Hongyang, Ma, Ben, Hagenmueller, Marco, Riffel, Johannes H., Meryer, Soeren, Zhang, Min, Hardt, Stefan E., Wang, Lin, Wang, Dao Wen, Qiu, Hongyu, Zhou, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790446/
https://www.ncbi.nlm.nih.gov/pubmed/29423029
http://dx.doi.org/10.18632/oncotarget.23149
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author Fan, Jingjing
Qiu, Lin
Shu, Hongyang
Ma, Ben
Hagenmueller, Marco
Riffel, Johannes H.
Meryer, Soeren
Zhang, Min
Hardt, Stefan E.
Wang, Lin
Wang, Dao Wen
Qiu, Hongyu
Zhou, Ning
author_facet Fan, Jingjing
Qiu, Lin
Shu, Hongyang
Ma, Ben
Hagenmueller, Marco
Riffel, Johannes H.
Meryer, Soeren
Zhang, Min
Hardt, Stefan E.
Wang, Lin
Wang, Dao Wen
Qiu, Hongyu
Zhou, Ning
author_sort Fan, Jingjing
collection PubMed
description Postinfarct cardiac hypertrophy is an independent risk factor for heart failure and sudden death. Regression of cardiac hypertrophy has emerged as a promising strategy in the treatment of myocardial infarction (MI). Here we hypothesized that frizzled1 (FZD1), a receptor of the canonical Wnt signaling pathway, is a novel mediator of ischemia-associated cardiac hypertrophy. MI was induced in mice by left anterior descending (LAD) coronary occlusion. One week after MI, the expression of FZD1 was found to be notably increased in the left ventricles (LVs) of the MI-mice compared to shams. Mouse recombinant FZD1 protein (RFP) was subcutaneously injected in the mice to provoke autoimmunization response. Anti-FZD1 antibody titer was significantly increased in the plasma of RFP-treated mice. RFP significantly mitigated the MI-induced cardiac hypertrophy and improved cardiac function in the MI mouse hearts. Moreover, increased heart and LV weights, myocardial size and the expression of β-myosin heavy chain in the MI-mice were also found to be attenuated by RFP. FZD1 was found to be significantly up-regulated in hypoxia-treated neonatal rat cardiomyocytes (NRCMs). Silencing FZD1 by siRNA transfection notably repressed the hypoxia-induced myocardial hypertrophy in NRCMs. Mechanistically, activation of canonical Wnt signaling induced by MI, e.g., β-catenin and glycogen synthase kinase-3β, was restrained in the LVs of the MI-mice treated by RFP, these inhibition on canonical Wnt signaling was further confirmed in hypoxic NRCMs transfected with FZD1 siRNA. In conclusion, immunization of RFP attenuated cardiac hypertrophy and improved cardiac function in the MI mice via blocking the canonical Wnt signaling pathway.
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spelling pubmed-57904462018-02-08 Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway Fan, Jingjing Qiu, Lin Shu, Hongyang Ma, Ben Hagenmueller, Marco Riffel, Johannes H. Meryer, Soeren Zhang, Min Hardt, Stefan E. Wang, Lin Wang, Dao Wen Qiu, Hongyu Zhou, Ning Oncotarget Research Paper Postinfarct cardiac hypertrophy is an independent risk factor for heart failure and sudden death. Regression of cardiac hypertrophy has emerged as a promising strategy in the treatment of myocardial infarction (MI). Here we hypothesized that frizzled1 (FZD1), a receptor of the canonical Wnt signaling pathway, is a novel mediator of ischemia-associated cardiac hypertrophy. MI was induced in mice by left anterior descending (LAD) coronary occlusion. One week after MI, the expression of FZD1 was found to be notably increased in the left ventricles (LVs) of the MI-mice compared to shams. Mouse recombinant FZD1 protein (RFP) was subcutaneously injected in the mice to provoke autoimmunization response. Anti-FZD1 antibody titer was significantly increased in the plasma of RFP-treated mice. RFP significantly mitigated the MI-induced cardiac hypertrophy and improved cardiac function in the MI mouse hearts. Moreover, increased heart and LV weights, myocardial size and the expression of β-myosin heavy chain in the MI-mice were also found to be attenuated by RFP. FZD1 was found to be significantly up-regulated in hypoxia-treated neonatal rat cardiomyocytes (NRCMs). Silencing FZD1 by siRNA transfection notably repressed the hypoxia-induced myocardial hypertrophy in NRCMs. Mechanistically, activation of canonical Wnt signaling induced by MI, e.g., β-catenin and glycogen synthase kinase-3β, was restrained in the LVs of the MI-mice treated by RFP, these inhibition on canonical Wnt signaling was further confirmed in hypoxic NRCMs transfected with FZD1 siRNA. In conclusion, immunization of RFP attenuated cardiac hypertrophy and improved cardiac function in the MI mice via blocking the canonical Wnt signaling pathway. Impact Journals LLC 2017-12-12 /pmc/articles/PMC5790446/ /pubmed/29423029 http://dx.doi.org/10.18632/oncotarget.23149 Text en Copyright: © 2018 Fan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Fan, Jingjing
Qiu, Lin
Shu, Hongyang
Ma, Ben
Hagenmueller, Marco
Riffel, Johannes H.
Meryer, Soeren
Zhang, Min
Hardt, Stefan E.
Wang, Lin
Wang, Dao Wen
Qiu, Hongyu
Zhou, Ning
Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title_full Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title_fullStr Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title_full_unstemmed Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title_short Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway
title_sort recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical wnt signaling pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790446/
https://www.ncbi.nlm.nih.gov/pubmed/29423029
http://dx.doi.org/10.18632/oncotarget.23149
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