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Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains

Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar(®) 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on th...

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Autores principales: Rachedi, Kounouz, Zermane, Ferial, Tir, Radja, Ayache, Fatima, Duran, Robert, Lauga, Béatrice, Karama, Solange, Simon, Maryse, Boulahrouf, Abderrahmane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790578/
https://www.ncbi.nlm.nih.gov/pubmed/28844884
http://dx.doi.org/10.1016/j.bjm.2017.05.004
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author Rachedi, Kounouz
Zermane, Ferial
Tir, Radja
Ayache, Fatima
Duran, Robert
Lauga, Béatrice
Karama, Solange
Simon, Maryse
Boulahrouf, Abderrahmane
author_facet Rachedi, Kounouz
Zermane, Ferial
Tir, Radja
Ayache, Fatima
Duran, Robert
Lauga, Béatrice
Karama, Solange
Simon, Maryse
Boulahrouf, Abderrahmane
author_sort Rachedi, Kounouz
collection PubMed
description Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar(®) 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar(®) herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances. Using a selective medium for Actinobacteria, 42 strains were isolated from both untreated and Granstar(®) treated soils. The number of isolates recovered from the treated agricultural soil was fewer than that isolated from the corresponding untreated soil, suggesting a negative effect of Granstar(®) herbicide on Actinobacteria community. Even so, the number of strains isolated from untreated and treated forest soil was quite similar. Among the isolates, resistant strains, tolerating high doses of Granstar(®) ranging from 0.3 to 0.6% (v/v), were obtained. The two most resistant strains (SRK12 and SRK17) were isolated from treated soils showing the importance of prior exposure to herbicides for bacterial adaptation. SRK12 and SRK17 strains showed different morphological features. The phylogenetic analysis, based on 16S rRNA gene sequencing, clustered the SRK12 strain with four Streptomyces type strains (S. vinaceusdrappus, S. mutabilis, S. ghanaensis and S. enissocaesilis), while SRK17 strain was closely related to Streptomyces africanus. Both strains were unable to grow on tribenuron methyl as unique source of carbon, despite its advanced dissipation. On the other hand, when glucose was added to tribenuron methyl, the bacterial development was evident with even an improvement of the tribenuron methyl degradation. In all cases, as tribenuron methyl disappeared, two compounds were detected with increased concentrations. These by-products appeared to be persistent and were not degraded either chemically or by the studied strains. Based on these observations, we suggested that bacterial activity on carbon substrates could be directly involved in the partial breakdown of tribenuron methyl, by generating the required acidity for the first step of the hydrolysis. Such a process would be interesting to consider in bioremediation of neutral and alkaline tribenuron methyl-polluted soils.
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spelling pubmed-57905782018-01-31 Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains Rachedi, Kounouz Zermane, Ferial Tir, Radja Ayache, Fatima Duran, Robert Lauga, Béatrice Karama, Solange Simon, Maryse Boulahrouf, Abderrahmane Braz J Microbiol Research Paper Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar(®) 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar(®) herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances. Using a selective medium for Actinobacteria, 42 strains were isolated from both untreated and Granstar(®) treated soils. The number of isolates recovered from the treated agricultural soil was fewer than that isolated from the corresponding untreated soil, suggesting a negative effect of Granstar(®) herbicide on Actinobacteria community. Even so, the number of strains isolated from untreated and treated forest soil was quite similar. Among the isolates, resistant strains, tolerating high doses of Granstar(®) ranging from 0.3 to 0.6% (v/v), were obtained. The two most resistant strains (SRK12 and SRK17) were isolated from treated soils showing the importance of prior exposure to herbicides for bacterial adaptation. SRK12 and SRK17 strains showed different morphological features. The phylogenetic analysis, based on 16S rRNA gene sequencing, clustered the SRK12 strain with four Streptomyces type strains (S. vinaceusdrappus, S. mutabilis, S. ghanaensis and S. enissocaesilis), while SRK17 strain was closely related to Streptomyces africanus. Both strains were unable to grow on tribenuron methyl as unique source of carbon, despite its advanced dissipation. On the other hand, when glucose was added to tribenuron methyl, the bacterial development was evident with even an improvement of the tribenuron methyl degradation. In all cases, as tribenuron methyl disappeared, two compounds were detected with increased concentrations. These by-products appeared to be persistent and were not degraded either chemically or by the studied strains. Based on these observations, we suggested that bacterial activity on carbon substrates could be directly involved in the partial breakdown of tribenuron methyl, by generating the required acidity for the first step of the hydrolysis. Such a process would be interesting to consider in bioremediation of neutral and alkaline tribenuron methyl-polluted soils. Elsevier 2017-08-09 /pmc/articles/PMC5790578/ /pubmed/28844884 http://dx.doi.org/10.1016/j.bjm.2017.05.004 Text en © 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Rachedi, Kounouz
Zermane, Ferial
Tir, Radja
Ayache, Fatima
Duran, Robert
Lauga, Béatrice
Karama, Solange
Simon, Maryse
Boulahrouf, Abderrahmane
Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title_full Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title_fullStr Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title_full_unstemmed Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title_short Effect of sulfonylurea tribenuron methyl herbicide on soil Actinobacteria growth and characterization of resistant strains
title_sort effect of sulfonylurea tribenuron methyl herbicide on soil actinobacteria growth and characterization of resistant strains
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790578/
https://www.ncbi.nlm.nih.gov/pubmed/28844884
http://dx.doi.org/10.1016/j.bjm.2017.05.004
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