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A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG

Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to b...

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Autores principales: Assaf, Jean Claude, Atoui, Ali, Khoury, André El, Chokr, Ali, Louka, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790588/
https://www.ncbi.nlm.nih.gov/pubmed/28843807
http://dx.doi.org/10.1016/j.bjm.2017.05.003
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author Assaf, Jean Claude
Atoui, Ali
Khoury, André El
Chokr, Ali
Louka, Nicolas
author_facet Assaf, Jean Claude
Atoui, Ali
Khoury, André El
Chokr, Ali
Louka, Nicolas
author_sort Assaf, Jean Claude
collection PubMed
description Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18 h at 37 °C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was tested. Depending on the bacterial concentration and procedure used, the percentages of bound AFM1 by L. rhamnosus GG varied from as low as undetectable to as high as 63%. The highest reduction in the level of unbound AFM1 was recorded for the five washes procedure that involved heating and pipetting. Results also showed that binding was partially reversible and AFM1 was released after repeated washes. These findings highlight the effect of different treatments on the binding of AFM1 to L. rhamnosus GG in liquid matrix.
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spelling pubmed-57905882018-01-31 A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG Assaf, Jean Claude Atoui, Ali Khoury, André El Chokr, Ali Louka, Nicolas Braz J Microbiol Research Paper Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18 h at 37 °C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was tested. Depending on the bacterial concentration and procedure used, the percentages of bound AFM1 by L. rhamnosus GG varied from as low as undetectable to as high as 63%. The highest reduction in the level of unbound AFM1 was recorded for the five washes procedure that involved heating and pipetting. Results also showed that binding was partially reversible and AFM1 was released after repeated washes. These findings highlight the effect of different treatments on the binding of AFM1 to L. rhamnosus GG in liquid matrix. Elsevier 2017-08-06 /pmc/articles/PMC5790588/ /pubmed/28843807 http://dx.doi.org/10.1016/j.bjm.2017.05.003 Text en © 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Assaf, Jean Claude
Atoui, Ali
Khoury, André El
Chokr, Ali
Louka, Nicolas
A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title_full A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title_fullStr A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title_full_unstemmed A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title_short A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
title_sort comparative study of procedures for binding of aflatoxin m1 to lactobacillus rhamnosus gg
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790588/
https://www.ncbi.nlm.nih.gov/pubmed/28843807
http://dx.doi.org/10.1016/j.bjm.2017.05.003
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