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Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine()
The quenching interaction of atomoxetine (ATX) with bovine serum albumin (BSA) was studied in vitro under optimal physiological condition (pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and life...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790691/ https://www.ncbi.nlm.nih.gov/pubmed/29404031 http://dx.doi.org/10.1016/j.jpha.2016.10.001 |
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author | Buddanavar, Arunkumar T. Nandibewoor, Sharanappa T. |
author_facet | Buddanavar, Arunkumar T. Nandibewoor, Sharanappa T. |
author_sort | Buddanavar, Arunkumar T. |
collection | PubMed |
description | The quenching interaction of atomoxetine (ATX) with bovine serum albumin (BSA) was studied in vitro under optimal physiological condition (pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ∆H° and ∆S° indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by Försters theory. UV-absorption, Fourier transform infrared spectroscopy (FT-IR), circular dichroism (CD), synchronous spectra and three-dimensional (3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied. |
format | Online Article Text |
id | pubmed-5790691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57906912018-02-05 Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() Buddanavar, Arunkumar T. Nandibewoor, Sharanappa T. J Pharm Anal Original Research Article The quenching interaction of atomoxetine (ATX) with bovine serum albumin (BSA) was studied in vitro under optimal physiological condition (pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ∆H° and ∆S° indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by Försters theory. UV-absorption, Fourier transform infrared spectroscopy (FT-IR), circular dichroism (CD), synchronous spectra and three-dimensional (3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied. Xi'an Jiaotong University 2017-06 2016-10-25 /pmc/articles/PMC5790691/ /pubmed/29404031 http://dx.doi.org/10.1016/j.jpha.2016.10.001 Text en © 2017 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article Buddanavar, Arunkumar T. Nandibewoor, Sharanappa T. Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title_full | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title_fullStr | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title_full_unstemmed | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title_short | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
title_sort | multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine() |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790691/ https://www.ncbi.nlm.nih.gov/pubmed/29404031 http://dx.doi.org/10.1016/j.jpha.2016.10.001 |
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