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Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice

Stress is the most frequently self-reported seizure precipitant in patients with epilepsy. Moreover, a relation between ear stress and epilepsy has been suggested. Although ear stress and stress hormones are known to influence seizure threshold in rodents, effects on the development of epilepsy (epi...

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Autores principales: van Campen, Jolien S., Hessel, Ellen V. S., Bohmbach, Kirsten, Rizzi, Giorgio, Lucassen, Paul J., Lakshmi Turimella, Sada, Umeoka, Eduardo H. L., Meerhoff, Gideon F., Braun, Kees P. J., de Graan, Pierre N. E., Joëls, Marian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790804/
https://www.ncbi.nlm.nih.gov/pubmed/29434572
http://dx.doi.org/10.3389/fendo.2018.00003
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author van Campen, Jolien S.
Hessel, Ellen V. S.
Bohmbach, Kirsten
Rizzi, Giorgio
Lucassen, Paul J.
Lakshmi Turimella, Sada
Umeoka, Eduardo H. L.
Meerhoff, Gideon F.
Braun, Kees P. J.
de Graan, Pierre N. E.
Joëls, Marian
author_facet van Campen, Jolien S.
Hessel, Ellen V. S.
Bohmbach, Kirsten
Rizzi, Giorgio
Lucassen, Paul J.
Lakshmi Turimella, Sada
Umeoka, Eduardo H. L.
Meerhoff, Gideon F.
Braun, Kees P. J.
de Graan, Pierre N. E.
Joëls, Marian
author_sort van Campen, Jolien S.
collection PubMed
description Stress is the most frequently self-reported seizure precipitant in patients with epilepsy. Moreover, a relation between ear stress and epilepsy has been suggested. Although ear stress and stress hormones are known to influence seizure threshold in rodents, effects on the development of epilepsy (epileptogenesis) are still unclear. Therefore, we studied the consequences of ear corticosteroid exposure for epileptogenesis, under highly controlled conditions in an animal model. Experimental febrile seizures (eFS) were elicited in 10-day-old mice by warm-air induced hyperthermia, while a control group was exposed to a normothermic condition. In the following 2 weeks, mice received either seven corticosterone or vehicle injections or were left undisturbed. Specific measures indicative for epileptogenesis were examined at 25 days of age and compared with vehicle injected or untreated mice. We examined structural [neurogenesis, dendritic morphology, and mossy fiber sprouting (MFS)] and functional (glutamatergic postsynaptic currents and long-term potentiation) plasticity in the dentate gyrus (DG). We found that differences in DG morphology induced by eFS were aggravated by repetitive (mildly stressful) vehicle injections and corticosterone exposure. In the injected groups, eFS were associated with decreases in neurogenesis, and increases in cell proliferation, dendritic length, and spine density. No group differences were found in MFS. Despite these changes in DG morphology, no effects of eFS were found on functional plasticity. We conclude that corticosterone exposure during early epileptogenesis elicited by eFS aggravates morphological, but not functional, changes in the DG, which partly supports the hypothesis that ear stress stimulates epileptogenesis.
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spelling pubmed-57908042018-02-12 Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice van Campen, Jolien S. Hessel, Ellen V. S. Bohmbach, Kirsten Rizzi, Giorgio Lucassen, Paul J. Lakshmi Turimella, Sada Umeoka, Eduardo H. L. Meerhoff, Gideon F. Braun, Kees P. J. de Graan, Pierre N. E. Joëls, Marian Front Endocrinol (Lausanne) Endocrinology Stress is the most frequently self-reported seizure precipitant in patients with epilepsy. Moreover, a relation between ear stress and epilepsy has been suggested. Although ear stress and stress hormones are known to influence seizure threshold in rodents, effects on the development of epilepsy (epileptogenesis) are still unclear. Therefore, we studied the consequences of ear corticosteroid exposure for epileptogenesis, under highly controlled conditions in an animal model. Experimental febrile seizures (eFS) were elicited in 10-day-old mice by warm-air induced hyperthermia, while a control group was exposed to a normothermic condition. In the following 2 weeks, mice received either seven corticosterone or vehicle injections or were left undisturbed. Specific measures indicative for epileptogenesis were examined at 25 days of age and compared with vehicle injected or untreated mice. We examined structural [neurogenesis, dendritic morphology, and mossy fiber sprouting (MFS)] and functional (glutamatergic postsynaptic currents and long-term potentiation) plasticity in the dentate gyrus (DG). We found that differences in DG morphology induced by eFS were aggravated by repetitive (mildly stressful) vehicle injections and corticosterone exposure. In the injected groups, eFS were associated with decreases in neurogenesis, and increases in cell proliferation, dendritic length, and spine density. No group differences were found in MFS. Despite these changes in DG morphology, no effects of eFS were found on functional plasticity. We conclude that corticosterone exposure during early epileptogenesis elicited by eFS aggravates morphological, but not functional, changes in the DG, which partly supports the hypothesis that ear stress stimulates epileptogenesis. Frontiers Media S.A. 2018-01-26 /pmc/articles/PMC5790804/ /pubmed/29434572 http://dx.doi.org/10.3389/fendo.2018.00003 Text en Copyright © 2018 van Campen, Hessel, Bohmbach, Rizzi, Lucassen, Lakshmi Turimella, Umeoka, Meerhoff, Braun, de Graan and Joëls. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
van Campen, Jolien S.
Hessel, Ellen V. S.
Bohmbach, Kirsten
Rizzi, Giorgio
Lucassen, Paul J.
Lakshmi Turimella, Sada
Umeoka, Eduardo H. L.
Meerhoff, Gideon F.
Braun, Kees P. J.
de Graan, Pierre N. E.
Joëls, Marian
Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title_full Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title_fullStr Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title_full_unstemmed Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title_short Stress and Corticosteroids Aggravate Morphological Changes in the Dentate Gyrus after Early-Life Experimental Febrile Seizures in Mice
title_sort stress and corticosteroids aggravate morphological changes in the dentate gyrus after early-life experimental febrile seizures in mice
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790804/
https://www.ncbi.nlm.nih.gov/pubmed/29434572
http://dx.doi.org/10.3389/fendo.2018.00003
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