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Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii

In this study, we aimed to synthesize silver nanoparticles containing fucans from Dictyota mertensii (Martius) Kützing using an environmentally friendly method and to characterize their structure as well as antiproliferative, immunomodulatory, and antibacterial effects. Fucan-coated silver nanoparti...

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Autores principales: Fernandes-Negreiros, Marília Medeiros, Araújo Machado, Raynara Iusk, Bezerra, Fabiana Lima, Nunes Melo, Maria Celeste, Alves, Monique Gabriela Chagas Faustino, Alves Filgueira, Luciana Guimaraes, Morgano, Marcelo Antonio, Trindade, Edvaldo Silva, Costa, Leandro Silva, Rocha, Hugo Alexandre Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791093/
https://www.ncbi.nlm.nih.gov/pubmed/29295570
http://dx.doi.org/10.3390/nano8010006
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author Fernandes-Negreiros, Marília Medeiros
Araújo Machado, Raynara Iusk
Bezerra, Fabiana Lima
Nunes Melo, Maria Celeste
Alves, Monique Gabriela Chagas Faustino
Alves Filgueira, Luciana Guimaraes
Morgano, Marcelo Antonio
Trindade, Edvaldo Silva
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
author_facet Fernandes-Negreiros, Marília Medeiros
Araújo Machado, Raynara Iusk
Bezerra, Fabiana Lima
Nunes Melo, Maria Celeste
Alves, Monique Gabriela Chagas Faustino
Alves Filgueira, Luciana Guimaraes
Morgano, Marcelo Antonio
Trindade, Edvaldo Silva
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
author_sort Fernandes-Negreiros, Marília Medeiros
collection PubMed
description In this study, we aimed to synthesize silver nanoparticles containing fucans from Dictyota mertensii (Martius) Kützing using an environmentally friendly method and to characterize their structure as well as antiproliferative, immunomodulatory, and antibacterial effects. Fucan-coated silver nanoparticles (FN) were characterized by Fourier-transform infrared analysis, dynamic light scattering, zeta potential, atomic force microscopy, energy dispersive X-ray spectroscopy, and inductively coupled plasma emission spectrometry. They were evaluated for their effect on cell viability, minimum inhibitory bactericidal concentration, and release of nitric oxide and cytokines. The FN were successfully synthesized using an environmentally friendly method. They were size-stable for 16 months, of a spherical shape, negative charge (−19.1 mV), and an average size of 103.3 ± 43 nm. They were able to inhibit the proliferation of the melanoma tumor cell line B16F10 (60%). In addition, they had immunomodulatory properties: they caused an up to 7000-fold increase in the release of nitric oxide and cytokines (IL-10; IL-6 and TNF-α) up to 7000 times. In addition, the FN showed inhibitory effect on Gram-positive and -negative bacteria, with MIC values of 50 µg/mL. Overall, the data showed that FN are nanoparticles with the potential to be used as antitumor, immunomodulatory, and antibacterial agents.
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spelling pubmed-57910932018-02-05 Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii Fernandes-Negreiros, Marília Medeiros Araújo Machado, Raynara Iusk Bezerra, Fabiana Lima Nunes Melo, Maria Celeste Alves, Monique Gabriela Chagas Faustino Alves Filgueira, Luciana Guimaraes Morgano, Marcelo Antonio Trindade, Edvaldo Silva Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira Nanomaterials (Basel) Article In this study, we aimed to synthesize silver nanoparticles containing fucans from Dictyota mertensii (Martius) Kützing using an environmentally friendly method and to characterize their structure as well as antiproliferative, immunomodulatory, and antibacterial effects. Fucan-coated silver nanoparticles (FN) were characterized by Fourier-transform infrared analysis, dynamic light scattering, zeta potential, atomic force microscopy, energy dispersive X-ray spectroscopy, and inductively coupled plasma emission spectrometry. They were evaluated for their effect on cell viability, minimum inhibitory bactericidal concentration, and release of nitric oxide and cytokines. The FN were successfully synthesized using an environmentally friendly method. They were size-stable for 16 months, of a spherical shape, negative charge (−19.1 mV), and an average size of 103.3 ± 43 nm. They were able to inhibit the proliferation of the melanoma tumor cell line B16F10 (60%). In addition, they had immunomodulatory properties: they caused an up to 7000-fold increase in the release of nitric oxide and cytokines (IL-10; IL-6 and TNF-α) up to 7000 times. In addition, the FN showed inhibitory effect on Gram-positive and -negative bacteria, with MIC values of 50 µg/mL. Overall, the data showed that FN are nanoparticles with the potential to be used as antitumor, immunomodulatory, and antibacterial agents. MDPI 2017-12-25 /pmc/articles/PMC5791093/ /pubmed/29295570 http://dx.doi.org/10.3390/nano8010006 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandes-Negreiros, Marília Medeiros
Araújo Machado, Raynara Iusk
Bezerra, Fabiana Lima
Nunes Melo, Maria Celeste
Alves, Monique Gabriela Chagas Faustino
Alves Filgueira, Luciana Guimaraes
Morgano, Marcelo Antonio
Trindade, Edvaldo Silva
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title_full Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title_fullStr Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title_full_unstemmed Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title_short Antibacterial, Antiproliferative, and Immunomodulatory Activity of Silver Nanoparticles Synthesized with Fucans from the Alga Dictyota mertensii
title_sort antibacterial, antiproliferative, and immunomodulatory activity of silver nanoparticles synthesized with fucans from the alga dictyota mertensii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791093/
https://www.ncbi.nlm.nih.gov/pubmed/29295570
http://dx.doi.org/10.3390/nano8010006
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