Cargando…
Primordial germ cell-mediated transgenesis and genome editing in birds
Transgenesis and genome editing in birds are based on a unique germline transmission system using primordial germ cells (PGCs), which is quite different from the mammalian transgenic and genome editing system. PGCs are progenitor cells of gametes that can deliver genetic information to the next gene...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791193/ https://www.ncbi.nlm.nih.gov/pubmed/29423217 http://dx.doi.org/10.1186/s40104-018-0234-4 |
_version_ | 1783296581080973312 |
---|---|
author | Han, Jae Yong Park, Young Hyun |
author_facet | Han, Jae Yong Park, Young Hyun |
author_sort | Han, Jae Yong |
collection | PubMed |
description | Transgenesis and genome editing in birds are based on a unique germline transmission system using primordial germ cells (PGCs), which is quite different from the mammalian transgenic and genome editing system. PGCs are progenitor cells of gametes that can deliver genetic information to the next generation. Since avian PGCs were first discovered in nineteenth century, there have been numerous efforts to reveal their origin, specification, and unique migration pattern, and to improve germline transmission efficiency. Recent advances in the isolation and in vitro culture of avian PGCs with genetic manipulation and genome editing tools enable the development of valuable avian models that were unavailable before. However, many challenges remain in the production of transgenic and genome-edited birds, including the precise control of germline transmission, introduction of exogenous genes, and genome editing in PGCs. Therefore, establishing reliable germline-competent PGCs and applying precise genome editing systems are critical current issues in the production of avian models. Here, we introduce a historical overview of avian PGCs and their application, including improved techniques and methodologies in the production of transgenic and genome-edited birds, and we discuss the future potential applications of transgenic and genome-edited birds to provide opportunities and benefits for humans. |
format | Online Article Text |
id | pubmed-5791193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57911932018-02-08 Primordial germ cell-mediated transgenesis and genome editing in birds Han, Jae Yong Park, Young Hyun J Anim Sci Biotechnol Review Transgenesis and genome editing in birds are based on a unique germline transmission system using primordial germ cells (PGCs), which is quite different from the mammalian transgenic and genome editing system. PGCs are progenitor cells of gametes that can deliver genetic information to the next generation. Since avian PGCs were first discovered in nineteenth century, there have been numerous efforts to reveal their origin, specification, and unique migration pattern, and to improve germline transmission efficiency. Recent advances in the isolation and in vitro culture of avian PGCs with genetic manipulation and genome editing tools enable the development of valuable avian models that were unavailable before. However, many challenges remain in the production of transgenic and genome-edited birds, including the precise control of germline transmission, introduction of exogenous genes, and genome editing in PGCs. Therefore, establishing reliable germline-competent PGCs and applying precise genome editing systems are critical current issues in the production of avian models. Here, we introduce a historical overview of avian PGCs and their application, including improved techniques and methodologies in the production of transgenic and genome-edited birds, and we discuss the future potential applications of transgenic and genome-edited birds to provide opportunities and benefits for humans. BioMed Central 2018-01-31 /pmc/articles/PMC5791193/ /pubmed/29423217 http://dx.doi.org/10.1186/s40104-018-0234-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Han, Jae Yong Park, Young Hyun Primordial germ cell-mediated transgenesis and genome editing in birds |
title | Primordial germ cell-mediated transgenesis and genome editing in birds |
title_full | Primordial germ cell-mediated transgenesis and genome editing in birds |
title_fullStr | Primordial germ cell-mediated transgenesis and genome editing in birds |
title_full_unstemmed | Primordial germ cell-mediated transgenesis and genome editing in birds |
title_short | Primordial germ cell-mediated transgenesis and genome editing in birds |
title_sort | primordial germ cell-mediated transgenesis and genome editing in birds |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791193/ https://www.ncbi.nlm.nih.gov/pubmed/29423217 http://dx.doi.org/10.1186/s40104-018-0234-4 |
work_keys_str_mv | AT hanjaeyong primordialgermcellmediatedtransgenesisandgenomeeditinginbirds AT parkyounghyun primordialgermcellmediatedtransgenesisandgenomeeditinginbirds |