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Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells
Thyroid stimulating hormone (TSH) stimulates the secretion of thyroid hormones by binding the TSH receptor (TSHR). TSHR is well-known to be expressed in thyroid tissue, excepting it, TSHR has also been expressed in many other tissues. In this study, we have examined the expression of TSHR in rat pan...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792451/ https://www.ncbi.nlm.nih.gov/pubmed/29386586 http://dx.doi.org/10.1038/s41598-018-20449-3 |
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author | Lyu, Jingya Imachi, Hitomi Yoshimoto, Takuo Fukunaga, Kensaku Sato, Seisuke Ibata, Tomohiro Kobayashi, Toshihiro Dong, Tao Yonezaki, Kazuko Yamaji, Nao Kikuchi, Fumi Iwama, Hisakazu Ishikawa, Ryou Haba, Reiji Sugiyama, Yasunori Zhang, Huanxiang Murao, Koji |
author_facet | Lyu, Jingya Imachi, Hitomi Yoshimoto, Takuo Fukunaga, Kensaku Sato, Seisuke Ibata, Tomohiro Kobayashi, Toshihiro Dong, Tao Yonezaki, Kazuko Yamaji, Nao Kikuchi, Fumi Iwama, Hisakazu Ishikawa, Ryou Haba, Reiji Sugiyama, Yasunori Zhang, Huanxiang Murao, Koji |
author_sort | Lyu, Jingya |
collection | PubMed |
description | Thyroid stimulating hormone (TSH) stimulates the secretion of thyroid hormones by binding the TSH receptor (TSHR). TSHR is well-known to be expressed in thyroid tissue, excepting it, TSHR has also been expressed in many other tissues. In this study, we have examined the expression of TSHR in rat pancreatic islets and evaluated the role of TSH in regulating pancreas-specific gene expression. TSHR was confirmed to be expressed in rodent pancreatic islets and its cell line, INS-1 cells. TSH directly affected the glucose uptake in INS cells by up-regulating the expression of GLUT2, and furthermore this process was blocked by SB203580, the specific inhibitor of the p38 MAPK signaling pathway. Similarly, TSH stimulated GLUT2 promoter activity, while both a dominant-negative p38MAPK α isoform (p38MAPK α-DN) and the specific inhibitor for p38MAPK α abolished the stimulatory effect of TSH on GLUT2 promoter activity. Finally, INS-1 cells treated with TSH showed increased protein level of glucokinase and enhanced glucose-stimulated insulin secretion. Together, these results confirm that TSHR is expressed in INS-1 cells and rat pancreatic islets, and suggest that activation of the p38MAPK α might be required for TSH-induced GLUT2 gene transcription in pancreatic β cells. |
format | Online Article Text |
id | pubmed-5792451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57924512018-02-12 Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells Lyu, Jingya Imachi, Hitomi Yoshimoto, Takuo Fukunaga, Kensaku Sato, Seisuke Ibata, Tomohiro Kobayashi, Toshihiro Dong, Tao Yonezaki, Kazuko Yamaji, Nao Kikuchi, Fumi Iwama, Hisakazu Ishikawa, Ryou Haba, Reiji Sugiyama, Yasunori Zhang, Huanxiang Murao, Koji Sci Rep Article Thyroid stimulating hormone (TSH) stimulates the secretion of thyroid hormones by binding the TSH receptor (TSHR). TSHR is well-known to be expressed in thyroid tissue, excepting it, TSHR has also been expressed in many other tissues. In this study, we have examined the expression of TSHR in rat pancreatic islets and evaluated the role of TSH in regulating pancreas-specific gene expression. TSHR was confirmed to be expressed in rodent pancreatic islets and its cell line, INS-1 cells. TSH directly affected the glucose uptake in INS cells by up-regulating the expression of GLUT2, and furthermore this process was blocked by SB203580, the specific inhibitor of the p38 MAPK signaling pathway. Similarly, TSH stimulated GLUT2 promoter activity, while both a dominant-negative p38MAPK α isoform (p38MAPK α-DN) and the specific inhibitor for p38MAPK α abolished the stimulatory effect of TSH on GLUT2 promoter activity. Finally, INS-1 cells treated with TSH showed increased protein level of glucokinase and enhanced glucose-stimulated insulin secretion. Together, these results confirm that TSHR is expressed in INS-1 cells and rat pancreatic islets, and suggest that activation of the p38MAPK α might be required for TSH-induced GLUT2 gene transcription in pancreatic β cells. Nature Publishing Group UK 2018-01-31 /pmc/articles/PMC5792451/ /pubmed/29386586 http://dx.doi.org/10.1038/s41598-018-20449-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lyu, Jingya Imachi, Hitomi Yoshimoto, Takuo Fukunaga, Kensaku Sato, Seisuke Ibata, Tomohiro Kobayashi, Toshihiro Dong, Tao Yonezaki, Kazuko Yamaji, Nao Kikuchi, Fumi Iwama, Hisakazu Ishikawa, Ryou Haba, Reiji Sugiyama, Yasunori Zhang, Huanxiang Murao, Koji Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title | Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title_full | Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title_fullStr | Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title_full_unstemmed | Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title_short | Thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, INS-1 cells |
title_sort | thyroid stimulating hormone stimulates the expression of glucose transporter 2 via its receptor in pancreatic β cell line, ins-1 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792451/ https://www.ncbi.nlm.nih.gov/pubmed/29386586 http://dx.doi.org/10.1038/s41598-018-20449-3 |
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