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Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)

The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000(RAD) and 15000(RAD)) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome...

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Autores principales: Perelman, Polina L., Pichler, Rudolf, Gaggl, Anna, Larkin, Denis M., Raudsepp, Terje, Alshanbari, Fahad, Holl, Heather M., Brooks, Samantha A., Burger, Pamela A., Periasamy, Kathiravan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792482/
https://www.ncbi.nlm.nih.gov/pubmed/29386528
http://dx.doi.org/10.1038/s41598-018-20223-5
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author Perelman, Polina L.
Pichler, Rudolf
Gaggl, Anna
Larkin, Denis M.
Raudsepp, Terje
Alshanbari, Fahad
Holl, Heather M.
Brooks, Samantha A.
Burger, Pamela A.
Periasamy, Kathiravan
author_facet Perelman, Polina L.
Pichler, Rudolf
Gaggl, Anna
Larkin, Denis M.
Raudsepp, Terje
Alshanbari, Fahad
Holl, Heather M.
Brooks, Samantha A.
Burger, Pamela A.
Periasamy, Kathiravan
author_sort Perelman, Polina L.
collection PubMed
description The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000(RAD) and 15000(RAD)) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000(RAD) panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000(RAD) panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000(RAD) panel is an important high-resolution complement to the main 5000(RAD) panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools.
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spelling pubmed-57924822018-02-12 Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD) Perelman, Polina L. Pichler, Rudolf Gaggl, Anna Larkin, Denis M. Raudsepp, Terje Alshanbari, Fahad Holl, Heather M. Brooks, Samantha A. Burger, Pamela A. Periasamy, Kathiravan Sci Rep Article The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000(RAD) and 15000(RAD)) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000(RAD) panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000(RAD) panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000(RAD) panel is an important high-resolution complement to the main 5000(RAD) panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools. Nature Publishing Group UK 2018-01-31 /pmc/articles/PMC5792482/ /pubmed/29386528 http://dx.doi.org/10.1038/s41598-018-20223-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Perelman, Polina L.
Pichler, Rudolf
Gaggl, Anna
Larkin, Denis M.
Raudsepp, Terje
Alshanbari, Fahad
Holl, Heather M.
Brooks, Samantha A.
Burger, Pamela A.
Periasamy, Kathiravan
Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title_full Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title_fullStr Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title_full_unstemmed Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title_short Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000(RAD) and 15000(RAD)
title_sort construction of two whole genome radiation hybrid panels for dromedary (camelus dromedarius): 5000(rad) and 15000(rad)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792482/
https://www.ncbi.nlm.nih.gov/pubmed/29386528
http://dx.doi.org/10.1038/s41598-018-20223-5
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