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Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived fro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793062/ https://www.ncbi.nlm.nih.gov/pubmed/29304006 http://dx.doi.org/10.3390/md16010014 |
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author | Shin, Hee Jae Choi, Byeoung-Kyu Trinh, Phan Thi Hoai Lee, Hwa-Sun Kang, Jong Soon Van, Tran Thi Thanh Lee, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon |
author_facet | Shin, Hee Jae Choi, Byeoung-Kyu Trinh, Phan Thi Hoai Lee, Hwa-Sun Kang, Jong Soon Van, Tran Thi Thanh Lee, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon |
author_sort | Shin, Hee Jae |
collection | PubMed |
description | A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1–5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity. |
format | Online Article Text |
id | pubmed-5793062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57930622018-02-06 Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. Shin, Hee Jae Choi, Byeoung-Kyu Trinh, Phan Thi Hoai Lee, Hwa-Sun Kang, Jong Soon Van, Tran Thi Thanh Lee, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon Mar Drugs Article A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1–5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity. MDPI 2018-01-05 /pmc/articles/PMC5793062/ /pubmed/29304006 http://dx.doi.org/10.3390/md16010014 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shin, Hee Jae Choi, Byeoung-Kyu Trinh, Phan Thi Hoai Lee, Hwa-Sun Kang, Jong Soon Van, Tran Thi Thanh Lee, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title | Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title_full | Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title_fullStr | Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title_full_unstemmed | Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title_short | Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. |
title_sort | suppression of rankl-induced osteoclastogenesis by the metabolites from the marine fungus aspergillus flocculosus isolated from a sponge stylissa sp. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793062/ https://www.ncbi.nlm.nih.gov/pubmed/29304006 http://dx.doi.org/10.3390/md16010014 |
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