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Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived fro...

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Autores principales: Shin, Hee Jae, Choi, Byeoung-Kyu, Trinh, Phan Thi Hoai, Lee, Hwa-Sun, Kang, Jong Soon, Van, Tran Thi Thanh, Lee, Hyi-Seung, Lee, Jong Seok, Lee, Yeon-Ju, Lee, Jihoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793062/
https://www.ncbi.nlm.nih.gov/pubmed/29304006
http://dx.doi.org/10.3390/md16010014
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author Shin, Hee Jae
Choi, Byeoung-Kyu
Trinh, Phan Thi Hoai
Lee, Hwa-Sun
Kang, Jong Soon
Van, Tran Thi Thanh
Lee, Hyi-Seung
Lee, Jong Seok
Lee, Yeon-Ju
Lee, Jihoon
author_facet Shin, Hee Jae
Choi, Byeoung-Kyu
Trinh, Phan Thi Hoai
Lee, Hwa-Sun
Kang, Jong Soon
Van, Tran Thi Thanh
Lee, Hyi-Seung
Lee, Jong Seok
Lee, Yeon-Ju
Lee, Jihoon
author_sort Shin, Hee Jae
collection PubMed
description A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1–5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity.
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spelling pubmed-57930622018-02-06 Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp. Shin, Hee Jae Choi, Byeoung-Kyu Trinh, Phan Thi Hoai Lee, Hwa-Sun Kang, Jong Soon Van, Tran Thi Thanh Lee, Hyi-Seung Lee, Jong Seok Lee, Yeon-Ju Lee, Jihoon Mar Drugs Article A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 1–5 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity. MDPI 2018-01-05 /pmc/articles/PMC5793062/ /pubmed/29304006 http://dx.doi.org/10.3390/md16010014 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shin, Hee Jae
Choi, Byeoung-Kyu
Trinh, Phan Thi Hoai
Lee, Hwa-Sun
Kang, Jong Soon
Van, Tran Thi Thanh
Lee, Hyi-Seung
Lee, Jong Seok
Lee, Yeon-Ju
Lee, Jihoon
Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title_full Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title_fullStr Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title_full_unstemmed Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title_short Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.
title_sort suppression of rankl-induced osteoclastogenesis by the metabolites from the marine fungus aspergillus flocculosus isolated from a sponge stylissa sp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793062/
https://www.ncbi.nlm.nih.gov/pubmed/29304006
http://dx.doi.org/10.3390/md16010014
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