Cargando…
Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei
Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or “Crustins” and Type IIb or “Crustin-like”) possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in pe...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793079/ https://www.ncbi.nlm.nih.gov/pubmed/29337853 http://dx.doi.org/10.3390/md16010031 |
_version_ | 1783296872476049408 |
---|---|
author | Barreto, Cairé Coelho, Jaqueline da Rosa Yuan, Jianbo Xiang, Jianhai Perazzolo, Luciane Maria Rosa, Rafael Diego |
author_facet | Barreto, Cairé Coelho, Jaqueline da Rosa Yuan, Jianbo Xiang, Jianhai Perazzolo, Luciane Maria Rosa, Rafael Diego |
author_sort | Barreto, Cairé |
collection | PubMed |
description | Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or “Crustins” and Type IIb or “Crustin-like”) possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in penaeid shrimp. To gain insight into the molecular diversity of Type II crustins in penaeids, we identified and characterized a Type IIb crustin in Litopenaeus vannamei (Crustin-like Lv) and compared Type II crustins at both molecular and transcriptional levels. Although L. vannamei Type II crustins (Crustin Lv and Crustin-like Lv) are encoded by separate genes, they showed a similar tissue distribution (hemocytes and gills) and transcriptional response to the shrimp pathogens Vibrio harveyi and White spot syndrome virus (WSSV). As Crustin Lv, Crustin-like Lv transcripts were found to be present early in development, suggesting a maternal contribution to shrimp progeny. Altogether, our in silico and transcriptional data allowed to conclude that (1) each sub-type displays a specific amino acid signature at the C-terminal end holding both the cysteine-rich region and the whey acidic protein (WAP) domain, and that (2) shrimp Type II crustins evolved from a common ancestral gene that conserved a similar pattern of transcriptional regulation. |
format | Online Article Text |
id | pubmed-5793079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57930792018-02-06 Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei Barreto, Cairé Coelho, Jaqueline da Rosa Yuan, Jianbo Xiang, Jianhai Perazzolo, Luciane Maria Rosa, Rafael Diego Mar Drugs Article Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or “Crustins” and Type IIb or “Crustin-like”) possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in penaeid shrimp. To gain insight into the molecular diversity of Type II crustins in penaeids, we identified and characterized a Type IIb crustin in Litopenaeus vannamei (Crustin-like Lv) and compared Type II crustins at both molecular and transcriptional levels. Although L. vannamei Type II crustins (Crustin Lv and Crustin-like Lv) are encoded by separate genes, they showed a similar tissue distribution (hemocytes and gills) and transcriptional response to the shrimp pathogens Vibrio harveyi and White spot syndrome virus (WSSV). As Crustin Lv, Crustin-like Lv transcripts were found to be present early in development, suggesting a maternal contribution to shrimp progeny. Altogether, our in silico and transcriptional data allowed to conclude that (1) each sub-type displays a specific amino acid signature at the C-terminal end holding both the cysteine-rich region and the whey acidic protein (WAP) domain, and that (2) shrimp Type II crustins evolved from a common ancestral gene that conserved a similar pattern of transcriptional regulation. MDPI 2018-01-16 /pmc/articles/PMC5793079/ /pubmed/29337853 http://dx.doi.org/10.3390/md16010031 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barreto, Cairé Coelho, Jaqueline da Rosa Yuan, Jianbo Xiang, Jianhai Perazzolo, Luciane Maria Rosa, Rafael Diego Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title | Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title_full | Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title_fullStr | Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title_full_unstemmed | Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title_short | Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei |
title_sort | specific molecular signatures for type ii crustins in penaeid shrimp uncovered by the identification of crustin-like antimicrobial peptides in litopenaeus vannamei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793079/ https://www.ncbi.nlm.nih.gov/pubmed/29337853 http://dx.doi.org/10.3390/md16010031 |
work_keys_str_mv | AT barretocaire specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei AT coelhojaquelinedarosa specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei AT yuanjianbo specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei AT xiangjianhai specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei AT perazzololucianemaria specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei AT rosarafaeldiego specificmolecularsignaturesfortypeiicrustinsinpenaeidshrimpuncoveredbytheidentificationofcrustinlikeantimicrobialpeptidesinlitopenaeusvannamei |