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Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats
(1) Background and (2) Methods: A 14-day in vivo, multitoxic (pure mycotoxins) rat experiment was conducted with zearalenone (ZEA; 15 μg/animal/day), deoxynivalenol (DON; 30 μg/animal/day) and fumonisin B(1) (FB(1); 150 μg/animal/day), as individual mycotoxins, binary (FD, FZ and DZ) and ternary com...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793091/ https://www.ncbi.nlm.nih.gov/pubmed/29271890 http://dx.doi.org/10.3390/toxins10010004 |
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author | Szabó, András Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Bázár, György Kocsó, Dániel Ali, Omeralfaroug Kovács, Melinda |
author_facet | Szabó, András Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Bázár, György Kocsó, Dániel Ali, Omeralfaroug Kovács, Melinda |
author_sort | Szabó, András |
collection | PubMed |
description | (1) Background and (2) Methods: A 14-day in vivo, multitoxic (pure mycotoxins) rat experiment was conducted with zearalenone (ZEA; 15 μg/animal/day), deoxynivalenol (DON; 30 μg/animal/day) and fumonisin B(1) (FB(1); 150 μg/animal/day), as individual mycotoxins, binary (FD, FZ and DZ) and ternary combinations (FDZ), via gavage in 1 mL water boluses. (3) Results: Body weight was unaffected, while liver (ZEA↑ vs. DON) and kidney weight (ZEA↑ vs. FDZ) increased. Hepatocellular membrane lipid fatty acids (FAs) referred to ceramide synthesis disturbance (C20:0, C22:0), and decreased unsaturation (C22:5 n3 and unsat. index), mainly induced by DON and to a lesser extent by ZEA. The DON-FB(1) interaction was additive on C20:0 in liver lipids. In renal phospholipids, ZEA had the strongest effect on the FA profile, affecting the saturated (C18:0) and many n6 FAs; ZEA was in an antagonistic relationship with FB(1) (C18:0) or DON (C18:2 n6, C20:1 n9). Hepatic oxidative stress was the most expressed in FD (reduced glutathione and glutathione peroxidase), while the nephrotoxic effect was further supported by lipid peroxidation (malondialdehyde) in the DON treatment. (4) Conclusions: In vivo study results refer to multiple mycotoxin interactions on membrane FAs, antioxidants and lipid peroxidation compounds, needing further testing. |
format | Online Article Text |
id | pubmed-5793091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57930912018-02-06 Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats Szabó, András Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Bázár, György Kocsó, Dániel Ali, Omeralfaroug Kovács, Melinda Toxins (Basel) Article (1) Background and (2) Methods: A 14-day in vivo, multitoxic (pure mycotoxins) rat experiment was conducted with zearalenone (ZEA; 15 μg/animal/day), deoxynivalenol (DON; 30 μg/animal/day) and fumonisin B(1) (FB(1); 150 μg/animal/day), as individual mycotoxins, binary (FD, FZ and DZ) and ternary combinations (FDZ), via gavage in 1 mL water boluses. (3) Results: Body weight was unaffected, while liver (ZEA↑ vs. DON) and kidney weight (ZEA↑ vs. FDZ) increased. Hepatocellular membrane lipid fatty acids (FAs) referred to ceramide synthesis disturbance (C20:0, C22:0), and decreased unsaturation (C22:5 n3 and unsat. index), mainly induced by DON and to a lesser extent by ZEA. The DON-FB(1) interaction was additive on C20:0 in liver lipids. In renal phospholipids, ZEA had the strongest effect on the FA profile, affecting the saturated (C18:0) and many n6 FAs; ZEA was in an antagonistic relationship with FB(1) (C18:0) or DON (C18:2 n6, C20:1 n9). Hepatic oxidative stress was the most expressed in FD (reduced glutathione and glutathione peroxidase), while the nephrotoxic effect was further supported by lipid peroxidation (malondialdehyde) in the DON treatment. (4) Conclusions: In vivo study results refer to multiple mycotoxin interactions on membrane FAs, antioxidants and lipid peroxidation compounds, needing further testing. MDPI 2017-12-22 /pmc/articles/PMC5793091/ /pubmed/29271890 http://dx.doi.org/10.3390/toxins10010004 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szabó, András Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Bázár, György Kocsó, Dániel Ali, Omeralfaroug Kovács, Melinda Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title | Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title_full | Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title_fullStr | Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title_full_unstemmed | Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title_short | Individual and Combined Effects of Fumonisin B(1), Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats |
title_sort | individual and combined effects of fumonisin b(1), deoxynivalenol and zearalenone on the hepatic and renal membrane lipid integrity of rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793091/ https://www.ncbi.nlm.nih.gov/pubmed/29271890 http://dx.doi.org/10.3390/toxins10010004 |
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