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Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin

The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First,...

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Autores principales: Guzman, Jesus, Téné, Nathan, Touchard, Axel, Castillo, Denis, Belkhelfa, Haouaria, Haddioui-Hbabi, Laila, Treilhou, Michel, Sauvain, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793108/
https://www.ncbi.nlm.nih.gov/pubmed/29286296
http://dx.doi.org/10.3390/toxins10010021
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author Guzman, Jesus
Téné, Nathan
Touchard, Axel
Castillo, Denis
Belkhelfa, Haouaria
Haddioui-Hbabi, Laila
Treilhou, Michel
Sauvain, Michel
author_facet Guzman, Jesus
Téné, Nathan
Touchard, Axel
Castillo, Denis
Belkhelfa, Haouaria
Haddioui-Hbabi, Laila
Treilhou, Michel
Sauvain, Michel
author_sort Guzman, Jesus
collection PubMed
description The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First, the effects of synthetic bicarinalin have been tested against Helicobacter pylori: one ATCC strain, and forty-four isolated from stomach ulcer biopsies of Peruvian patients. Then the cytoxicity of bicarinalin on human gastric cells and murine peritoneal macrophages was measured using XTT and MTT assays, respectively. Finally, the preventive effect of bicarinalin was evaluated by scanning electron microscopy using an adherence assay of H. pylori on human gastric cells treated with bicarinalin. This peptide has a potent antibacterial activity at the same magnitude as four antibiotics currently used in therapies against H. pylori. Bicarinalin also inhibited adherence of H. pylori to gastric cells with an IC(50) of 0.12 μg·mL(−1) and had low toxicity for human cells. Scanning electron microscopy confirmed that bicarinalin can significantly decrease the density of H. pylori on gastric cells. We conclude that Bicarinalin is a promising compound for the development of a novel and effective anti-H. pylori agent for both curative and preventive use.
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spelling pubmed-57931082018-02-06 Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin Guzman, Jesus Téné, Nathan Touchard, Axel Castillo, Denis Belkhelfa, Haouaria Haddioui-Hbabi, Laila Treilhou, Michel Sauvain, Michel Toxins (Basel) Article The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First, the effects of synthetic bicarinalin have been tested against Helicobacter pylori: one ATCC strain, and forty-four isolated from stomach ulcer biopsies of Peruvian patients. Then the cytoxicity of bicarinalin on human gastric cells and murine peritoneal macrophages was measured using XTT and MTT assays, respectively. Finally, the preventive effect of bicarinalin was evaluated by scanning electron microscopy using an adherence assay of H. pylori on human gastric cells treated with bicarinalin. This peptide has a potent antibacterial activity at the same magnitude as four antibiotics currently used in therapies against H. pylori. Bicarinalin also inhibited adherence of H. pylori to gastric cells with an IC(50) of 0.12 μg·mL(−1) and had low toxicity for human cells. Scanning electron microscopy confirmed that bicarinalin can significantly decrease the density of H. pylori on gastric cells. We conclude that Bicarinalin is a promising compound for the development of a novel and effective anti-H. pylori agent for both curative and preventive use. MDPI 2017-12-29 /pmc/articles/PMC5793108/ /pubmed/29286296 http://dx.doi.org/10.3390/toxins10010021 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guzman, Jesus
Téné, Nathan
Touchard, Axel
Castillo, Denis
Belkhelfa, Haouaria
Haddioui-Hbabi, Laila
Treilhou, Michel
Sauvain, Michel
Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_full Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_fullStr Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_full_unstemmed Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_short Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_sort anti-helicobacter pylori properties of the ant-venom peptide bicarinalin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793108/
https://www.ncbi.nlm.nih.gov/pubmed/29286296
http://dx.doi.org/10.3390/toxins10010021
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