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Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes

The caenophidian (assigned also as “advanced”) snakes are traditionally viewed as a group of reptiles with a limited karyotypic variation and stable ZZ/ZW sex chromosomes. The W chromosomes of the caenophidian snakes are heterochromatic, and pioneering studies demonstrated that they are rich in repe...

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Autores principales: Augstenová, Barbora, Mazzoleni, Sofia, Kratochvíl, Lukáš, Rovatsos, Michail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793158/
https://www.ncbi.nlm.nih.gov/pubmed/29283388
http://dx.doi.org/10.3390/genes9010005
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author Augstenová, Barbora
Mazzoleni, Sofia
Kratochvíl, Lukáš
Rovatsos, Michail
author_facet Augstenová, Barbora
Mazzoleni, Sofia
Kratochvíl, Lukáš
Rovatsos, Michail
author_sort Augstenová, Barbora
collection PubMed
description The caenophidian (assigned also as “advanced”) snakes are traditionally viewed as a group of reptiles with a limited karyotypic variation and stable ZZ/ZW sex chromosomes. The W chromosomes of the caenophidian snakes are heterochromatic, and pioneering studies demonstrated that they are rich in repetitive elements. However, a comparative study of the evolutionary dynamics of the repetitive content of the W chromosome across the whole lineage is missing. Using molecular-cytogenetic techniques, we explored the distribution of four repetitive motifs (microsatellites GATA, GACA, AG and telomeric-like sequences), which are frequently accumulated in differentiated sex chromosomes in vertebrates, in the genomes of 13 species of the caenophidian snakes covering a wide phylogenetic spectrum of the lineage. The results demonstrate a striking variability in the morphology and the repetitive content of the W chromosomes even between closely-related species, which is in contrast to the homology and long-term stability of the gene content of the caenophidian Z chromosome. We uncovered that the tested microsatellite motifs are accumulated on the degenerated, heterochromatic W chromosomes in all tested species of the caenophidian snakes with the exception of the Javan file snake representing a basal clade. On the other hand, the presence of the accumulation of the telomeric-like sequences on the caenophidian W chromosome is evolutionary much less stable. Moreover, we demonstrated that large accumulations of telomeric-like motifs on the W chromosome contribute to sexual differences in the number of copies of the telomeric and telomeric-like repeats estimated by quantitative PCR, which might be confusing and incorrectly interpreted as sexual differences in telomere length.
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spelling pubmed-57931582018-02-07 Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes Augstenová, Barbora Mazzoleni, Sofia Kratochvíl, Lukáš Rovatsos, Michail Genes (Basel) Article The caenophidian (assigned also as “advanced”) snakes are traditionally viewed as a group of reptiles with a limited karyotypic variation and stable ZZ/ZW sex chromosomes. The W chromosomes of the caenophidian snakes are heterochromatic, and pioneering studies demonstrated that they are rich in repetitive elements. However, a comparative study of the evolutionary dynamics of the repetitive content of the W chromosome across the whole lineage is missing. Using molecular-cytogenetic techniques, we explored the distribution of four repetitive motifs (microsatellites GATA, GACA, AG and telomeric-like sequences), which are frequently accumulated in differentiated sex chromosomes in vertebrates, in the genomes of 13 species of the caenophidian snakes covering a wide phylogenetic spectrum of the lineage. The results demonstrate a striking variability in the morphology and the repetitive content of the W chromosomes even between closely-related species, which is in contrast to the homology and long-term stability of the gene content of the caenophidian Z chromosome. We uncovered that the tested microsatellite motifs are accumulated on the degenerated, heterochromatic W chromosomes in all tested species of the caenophidian snakes with the exception of the Javan file snake representing a basal clade. On the other hand, the presence of the accumulation of the telomeric-like sequences on the caenophidian W chromosome is evolutionary much less stable. Moreover, we demonstrated that large accumulations of telomeric-like motifs on the W chromosome contribute to sexual differences in the number of copies of the telomeric and telomeric-like repeats estimated by quantitative PCR, which might be confusing and incorrectly interpreted as sexual differences in telomere length. MDPI 2017-12-28 /pmc/articles/PMC5793158/ /pubmed/29283388 http://dx.doi.org/10.3390/genes9010005 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Augstenová, Barbora
Mazzoleni, Sofia
Kratochvíl, Lukáš
Rovatsos, Michail
Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title_full Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title_fullStr Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title_full_unstemmed Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title_short Evolutionary Dynamics of the W Chromosome in Caenophidian Snakes
title_sort evolutionary dynamics of the w chromosome in caenophidian snakes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793158/
https://www.ncbi.nlm.nih.gov/pubmed/29283388
http://dx.doi.org/10.3390/genes9010005
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