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Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein
BACKGROUND & OBJECTIVES: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. Howeve...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793473/ https://www.ncbi.nlm.nih.gov/pubmed/29355145 http://dx.doi.org/10.4103/ijmr.IJMR_291_16 |
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author | Sarmah, Nilanju Pran Sarma, Kishore Bhattacharyya, Dibya Ranjan Sultan, Ali Bansal, Devendra Singh, Neeru Bharti, Praveen K. Kaur, Hargobinder Sehgal, Rakesh Mohapatra, Pradyumna Kishore Mahanta, Jagadish |
author_facet | Sarmah, Nilanju Pran Sarma, Kishore Bhattacharyya, Dibya Ranjan Sultan, Ali Bansal, Devendra Singh, Neeru Bharti, Praveen K. Kaur, Hargobinder Sehgal, Rakesh Mohapatra, Pradyumna Kishore Mahanta, Jagadish |
author_sort | Sarmah, Nilanju Pran |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. METHODS: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. RESULTS: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63%), followed by MAD20 (29%) and K1 (8%) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. INTERPRETATION & CONCLUSIONS: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region. |
format | Online Article Text |
id | pubmed-5793473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57934732018-02-08 Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein Sarmah, Nilanju Pran Sarma, Kishore Bhattacharyya, Dibya Ranjan Sultan, Ali Bansal, Devendra Singh, Neeru Bharti, Praveen K. Kaur, Hargobinder Sehgal, Rakesh Mohapatra, Pradyumna Kishore Mahanta, Jagadish Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. METHODS: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. RESULTS: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63%), followed by MAD20 (29%) and K1 (8%) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. INTERPRETATION & CONCLUSIONS: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region. Medknow Publications & Media Pvt Ltd 2017-09 /pmc/articles/PMC5793473/ /pubmed/29355145 http://dx.doi.org/10.4103/ijmr.IJMR_291_16 Text en Copyright: © 2017 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sarmah, Nilanju Pran Sarma, Kishore Bhattacharyya, Dibya Ranjan Sultan, Ali Bansal, Devendra Singh, Neeru Bharti, Praveen K. Kaur, Hargobinder Sehgal, Rakesh Mohapatra, Pradyumna Kishore Mahanta, Jagadish Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title | Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title_full | Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title_fullStr | Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title_full_unstemmed | Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title_short | Molecular characterization of Plasmodium falciparum in Arunachal Pradesh from Northeast India based on merozoite surface protein 1 & glutamate-rich protein |
title_sort | molecular characterization of plasmodium falciparum in arunachal pradesh from northeast india based on merozoite surface protein 1 & glutamate-rich protein |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793473/ https://www.ncbi.nlm.nih.gov/pubmed/29355145 http://dx.doi.org/10.4103/ijmr.IJMR_291_16 |
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