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Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide
Graphene oxide (GO) is emerging as a promising nanomaterial with potential application in the detection and analysis of amino acids, DNA, enzymes, and proteins in biological fluid samples. So, the reaction of GO with amino acids should be characterized and determined before using it in biosensing me...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793566/ https://www.ncbi.nlm.nih.gov/pubmed/29300306 http://dx.doi.org/10.3390/ma11010068 |
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author | Nassef, Hossam M. Hagar, Mohamed Malek, Zeiad Othman, Abdelhameed M. |
author_facet | Nassef, Hossam M. Hagar, Mohamed Malek, Zeiad Othman, Abdelhameed M. |
author_sort | Nassef, Hossam M. |
collection | PubMed |
description | Graphene oxide (GO) is emerging as a promising nanomaterial with potential application in the detection and analysis of amino acids, DNA, enzymes, and proteins in biological fluid samples. So, the reaction of GO with amino acids should be characterized and determined before using it in biosensing methods and devices. In this study, the reaction of tyrosine amino acid (Tyr) with GO was characterized using FT-IR, UV-vis spectrophotometry, and scanning electron microscopy (SEM) before its use. The optimum conditions for GO’s interaction with Tyr amino acid have been studied under variable conditions. The optimum conditions of pH, temperature, shaking time, and GO and tyrosine concentrations for the uptaking of tyrosine amino acid onto the GO’s surface from aqueous solution were determined. The SEM analysis showed that the GO supplied was in a particle size range between 5.4 and 8.1 nm. A pH of 8.4–9.4 at 25 °C and 5 min of shaking time were the optimum conditions for a maximum uptake of 1.4 μg/mL of tyrosine amino acid onto 0.2 mg/mL of GO. |
format | Online Article Text |
id | pubmed-5793566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57935662018-02-07 Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide Nassef, Hossam M. Hagar, Mohamed Malek, Zeiad Othman, Abdelhameed M. Materials (Basel) Article Graphene oxide (GO) is emerging as a promising nanomaterial with potential application in the detection and analysis of amino acids, DNA, enzymes, and proteins in biological fluid samples. So, the reaction of GO with amino acids should be characterized and determined before using it in biosensing methods and devices. In this study, the reaction of tyrosine amino acid (Tyr) with GO was characterized using FT-IR, UV-vis spectrophotometry, and scanning electron microscopy (SEM) before its use. The optimum conditions for GO’s interaction with Tyr amino acid have been studied under variable conditions. The optimum conditions of pH, temperature, shaking time, and GO and tyrosine concentrations for the uptaking of tyrosine amino acid onto the GO’s surface from aqueous solution were determined. The SEM analysis showed that the GO supplied was in a particle size range between 5.4 and 8.1 nm. A pH of 8.4–9.4 at 25 °C and 5 min of shaking time were the optimum conditions for a maximum uptake of 1.4 μg/mL of tyrosine amino acid onto 0.2 mg/mL of GO. MDPI 2018-01-04 /pmc/articles/PMC5793566/ /pubmed/29300306 http://dx.doi.org/10.3390/ma11010068 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nassef, Hossam M. Hagar, Mohamed Malek, Zeiad Othman, Abdelhameed M. Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title | Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title_full | Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title_fullStr | Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title_full_unstemmed | Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title_short | Uptake of Tyrosine Amino Acid on Nano-Graphene Oxide |
title_sort | uptake of tyrosine amino acid on nano-graphene oxide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793566/ https://www.ncbi.nlm.nih.gov/pubmed/29300306 http://dx.doi.org/10.3390/ma11010068 |
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