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Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin

Nanoparticles or microparticles created by physical complexation between two polyelectrolytes may have a prospective use as an excipient for oral insulin administration. Natural polymers such as tragacanth, alginate, dextran, pullulan, hyaluronic acid, gelatin and chitosan can be potential candidate...

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Autores principales: Nur, Mokhamad, Vasiljevic, Todor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793577/
https://www.ncbi.nlm.nih.gov/pubmed/29304023
http://dx.doi.org/10.3390/ma11010079
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author Nur, Mokhamad
Vasiljevic, Todor
author_facet Nur, Mokhamad
Vasiljevic, Todor
author_sort Nur, Mokhamad
collection PubMed
description Nanoparticles or microparticles created by physical complexation between two polyelectrolytes may have a prospective use as an excipient for oral insulin administration. Natural polymers such as tragacanth, alginate, dextran, pullulan, hyaluronic acid, gelatin and chitosan can be potential candidates for this purpose. In this research, insulin particles were prepared by the inclusion of insulin into a tragacanth hydrogel. The effect of the pH and concentration relationship involving polyelectrolytes offering individual particle size and zeta potential was assessed by zetasizer and scanning electron microscopy (SEM). Insulin–tragacanth interactions at varying pH (3.7, 4.3, 4.6, or 6), and concentration (0.1%, 0.5%, or 1% w/w) were evaluated by differential scanning calorimetry (DSC) and ATR Fourier transform infrared (ATR-FTIR) analysis. Individual and smaller particles, approximately 800 nm, were acquired at pH 4.6 with 0.5% of tragacanth. The acid gelation test indicated that insulin could be entrapped in the physical hydrogel of tragacanth. DSC thermograms of insulin–tragacanth showed shifts on the same unloaded tragacanth peaks and suggested polyelectrolyte–protein interactions at a pH close to 4.3–4.6. FTIR spectra of tragacanth–insulin complexes exhibited amide absorption bands featuring in the protein spectra and revealed the creation of a new chemical substance.
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spelling pubmed-57935772018-02-07 Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin Nur, Mokhamad Vasiljevic, Todor Materials (Basel) Article Nanoparticles or microparticles created by physical complexation between two polyelectrolytes may have a prospective use as an excipient for oral insulin administration. Natural polymers such as tragacanth, alginate, dextran, pullulan, hyaluronic acid, gelatin and chitosan can be potential candidates for this purpose. In this research, insulin particles were prepared by the inclusion of insulin into a tragacanth hydrogel. The effect of the pH and concentration relationship involving polyelectrolytes offering individual particle size and zeta potential was assessed by zetasizer and scanning electron microscopy (SEM). Insulin–tragacanth interactions at varying pH (3.7, 4.3, 4.6, or 6), and concentration (0.1%, 0.5%, or 1% w/w) were evaluated by differential scanning calorimetry (DSC) and ATR Fourier transform infrared (ATR-FTIR) analysis. Individual and smaller particles, approximately 800 nm, were acquired at pH 4.6 with 0.5% of tragacanth. The acid gelation test indicated that insulin could be entrapped in the physical hydrogel of tragacanth. DSC thermograms of insulin–tragacanth showed shifts on the same unloaded tragacanth peaks and suggested polyelectrolyte–protein interactions at a pH close to 4.3–4.6. FTIR spectra of tragacanth–insulin complexes exhibited amide absorption bands featuring in the protein spectra and revealed the creation of a new chemical substance. MDPI 2018-01-05 /pmc/articles/PMC5793577/ /pubmed/29304023 http://dx.doi.org/10.3390/ma11010079 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nur, Mokhamad
Vasiljevic, Todor
Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title_full Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title_fullStr Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title_full_unstemmed Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title_short Insulin Inclusion into a Tragacanth Hydrogel: An Oral Delivery System for Insulin
title_sort insulin inclusion into a tragacanth hydrogel: an oral delivery system for insulin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793577/
https://www.ncbi.nlm.nih.gov/pubmed/29304023
http://dx.doi.org/10.3390/ma11010079
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