Cargando…

Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas

Common bean (Phaseolus vulgaris L.) is one of the most important grain legume crops in the world. The beans grown in north-western Himalayas possess huge diversity for seed color, shape and size but are mostly susceptible to Anthracnose disease caused by seed born fungus Colletotrichum lindemuthianu...

Descripción completa

Detalles Bibliográficos
Autores principales: Choudhary, Neeraj, Bawa, Vanya, Paliwal, Rajneesh, Singh, Bikram, Bhat, Mohd. Ashraf, Mir, Javid Iqbal, Gupta, Moni, Sofi, Parvaze A., Thudi, Mahendar, Varshney, Rajeev K., Mir, Reyazul Rouf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794095/
https://www.ncbi.nlm.nih.gov/pubmed/29389971
http://dx.doi.org/10.1371/journal.pone.0191700
_version_ 1783297059082731520
author Choudhary, Neeraj
Bawa, Vanya
Paliwal, Rajneesh
Singh, Bikram
Bhat, Mohd. Ashraf
Mir, Javid Iqbal
Gupta, Moni
Sofi, Parvaze A.
Thudi, Mahendar
Varshney, Rajeev K.
Mir, Reyazul Rouf
author_facet Choudhary, Neeraj
Bawa, Vanya
Paliwal, Rajneesh
Singh, Bikram
Bhat, Mohd. Ashraf
Mir, Javid Iqbal
Gupta, Moni
Sofi, Parvaze A.
Thudi, Mahendar
Varshney, Rajeev K.
Mir, Reyazul Rouf
author_sort Choudhary, Neeraj
collection PubMed
description Common bean (Phaseolus vulgaris L.) is one of the most important grain legume crops in the world. The beans grown in north-western Himalayas possess huge diversity for seed color, shape and size but are mostly susceptible to Anthracnose disease caused by seed born fungus Colletotrichum lindemuthianum. Dozens of QTLs/genes have been already identified for this disease in common bean world-wide. However, this is the first report of gene/QTL discovery for Anthracnose using bean germplasm from north-western Himalayas of state Jammu & Kashmir, India. A core set of 96 bean lines comprising 54 indigenous local landraces from 11 hot-spots and 42 exotic lines from 10 different countries were phenotyped at two locations (SKUAST-Jammu and Bhaderwah, Jammu) for Anthracnose resistance. The core set was also genotyped with genome-wide (91) random and trait linked SSR markers. The study of marker-trait associations (MTAs) led to the identification of 10 QTLs/genes for Anthracnose resistance. Among the 10 QTLs/genes identified, two MTAs are stable (BM45 & BM211), two MTAs (PVctt1 & BM211) are major explaining more than 20% phenotypic variation for Anthracnose and one MTA (BM211) is both stable and major. Six (06) genomic regions are reported for the first time, while as four (04) genomic regions validated the already known QTL/gene regions/clusters for Anthracnose. The major, stable and validated markers reported during the present study associated with Anthracnose resistance will prove useful in common bean molecular breeding programs aimed at enhancing Anthracnose resistance of local bean landraces grown in north-western Himalayas of state Jammu and Kashmir.
format Online
Article
Text
id pubmed-5794095
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-57940952018-02-16 Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas Choudhary, Neeraj Bawa, Vanya Paliwal, Rajneesh Singh, Bikram Bhat, Mohd. Ashraf Mir, Javid Iqbal Gupta, Moni Sofi, Parvaze A. Thudi, Mahendar Varshney, Rajeev K. Mir, Reyazul Rouf PLoS One Research Article Common bean (Phaseolus vulgaris L.) is one of the most important grain legume crops in the world. The beans grown in north-western Himalayas possess huge diversity for seed color, shape and size but are mostly susceptible to Anthracnose disease caused by seed born fungus Colletotrichum lindemuthianum. Dozens of QTLs/genes have been already identified for this disease in common bean world-wide. However, this is the first report of gene/QTL discovery for Anthracnose using bean germplasm from north-western Himalayas of state Jammu & Kashmir, India. A core set of 96 bean lines comprising 54 indigenous local landraces from 11 hot-spots and 42 exotic lines from 10 different countries were phenotyped at two locations (SKUAST-Jammu and Bhaderwah, Jammu) for Anthracnose resistance. The core set was also genotyped with genome-wide (91) random and trait linked SSR markers. The study of marker-trait associations (MTAs) led to the identification of 10 QTLs/genes for Anthracnose resistance. Among the 10 QTLs/genes identified, two MTAs are stable (BM45 & BM211), two MTAs (PVctt1 & BM211) are major explaining more than 20% phenotypic variation for Anthracnose and one MTA (BM211) is both stable and major. Six (06) genomic regions are reported for the first time, while as four (04) genomic regions validated the already known QTL/gene regions/clusters for Anthracnose. The major, stable and validated markers reported during the present study associated with Anthracnose resistance will prove useful in common bean molecular breeding programs aimed at enhancing Anthracnose resistance of local bean landraces grown in north-western Himalayas of state Jammu and Kashmir. Public Library of Science 2018-02-01 /pmc/articles/PMC5794095/ /pubmed/29389971 http://dx.doi.org/10.1371/journal.pone.0191700 Text en © 2018 Choudhary et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Choudhary, Neeraj
Bawa, Vanya
Paliwal, Rajneesh
Singh, Bikram
Bhat, Mohd. Ashraf
Mir, Javid Iqbal
Gupta, Moni
Sofi, Parvaze A.
Thudi, Mahendar
Varshney, Rajeev K.
Mir, Reyazul Rouf
Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title_full Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title_fullStr Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title_full_unstemmed Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title_short Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
title_sort gene/qtl discovery for anthracnose in common bean (phaseolus vulgaris l.) from north-western himalayas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794095/
https://www.ncbi.nlm.nih.gov/pubmed/29389971
http://dx.doi.org/10.1371/journal.pone.0191700
work_keys_str_mv AT choudharyneeraj geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT bawavanya geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT paliwalrajneesh geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT singhbikram geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT bhatmohdashraf geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT mirjavidiqbal geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT guptamoni geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT sofiparvazea geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT thudimahendar geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT varshneyrajeevk geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas
AT mirreyazulrouf geneqtldiscoveryforanthracnoseincommonbeanphaseolusvulgarislfromnorthwesternhimalayas