Cargando…

Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form

Photodynamic therapy is an advanced method of treating cancer and various benign diseases, including infections. It uses light‐activated molecules [photosensitizers (PSs)] to generate reactive oxygen species (ROS) when irradiated with light of a specific wavelength. This study examined the photophys...

Descripción completa

Detalles Bibliográficos
Autores principales: Kostryukova, Lyubov V., Prozorovskiy, Vladimir N., Medvedeva, Natalya V., Ipatova, Olga M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794465/
https://www.ncbi.nlm.nih.gov/pubmed/29435410
http://dx.doi.org/10.1002/2211-5463.12359
_version_ 1783297128838201344
author Kostryukova, Lyubov V.
Prozorovskiy, Vladimir N.
Medvedeva, Natalya V.
Ipatova, Olga M.
author_facet Kostryukova, Lyubov V.
Prozorovskiy, Vladimir N.
Medvedeva, Natalya V.
Ipatova, Olga M.
author_sort Kostryukova, Lyubov V.
collection PubMed
description Photodynamic therapy is an advanced method of treating cancer and various benign diseases, including infections. It uses light‐activated molecules [photosensitizers (PSs)] to generate reactive oxygen species (ROS) when irradiated with light of a specific wavelength. This study examined the photophysical and photosensitizing activity of the PS chlorin e6 incorporated in a delivery system based on plant phospholipids. This new nanoform of chlorin e6 comprised particles with a diameter of 18.4 ± 2.5 nm and zeta potential of −34.6 ± 3.0 mV. Incorporation of chlorin e6 in phospholipid nanoparticles was observed to cause a bathochromic shift of Q‐band absorption maximum by 14 nm without an absorption change in the range of the Soret band. Fluorescence intensity of chlorin e6 embedded in the phospholipid nanoparticles increased 1.7‐fold. Chlorin e6 in phospholipid nanoparticles, when irradiated, was able to generate ROS as shown by oxidation of polyunsaturated fatty acids of the phospholipid matrix of the delivery system and reduced l‐glutathione. In vivo it was demonstrated that the new nanoform of chlorin e6 provides more accumulation of PSs in tumor tissue than its free form. Moreover, its accumulation in the skin was lower and its elimination from the skin almost five times faster than when administered in free form. The observed differences of this new nanoform of chlorin e6 should lead to enhancement of antitumor efficacy and a decrease in phototoxicity.
format Online
Article
Text
id pubmed-5794465
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-57944652018-02-12 Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form Kostryukova, Lyubov V. Prozorovskiy, Vladimir N. Medvedeva, Natalya V. Ipatova, Olga M. FEBS Open Bio Research Articles Photodynamic therapy is an advanced method of treating cancer and various benign diseases, including infections. It uses light‐activated molecules [photosensitizers (PSs)] to generate reactive oxygen species (ROS) when irradiated with light of a specific wavelength. This study examined the photophysical and photosensitizing activity of the PS chlorin e6 incorporated in a delivery system based on plant phospholipids. This new nanoform of chlorin e6 comprised particles with a diameter of 18.4 ± 2.5 nm and zeta potential of −34.6 ± 3.0 mV. Incorporation of chlorin e6 in phospholipid nanoparticles was observed to cause a bathochromic shift of Q‐band absorption maximum by 14 nm without an absorption change in the range of the Soret band. Fluorescence intensity of chlorin e6 embedded in the phospholipid nanoparticles increased 1.7‐fold. Chlorin e6 in phospholipid nanoparticles, when irradiated, was able to generate ROS as shown by oxidation of polyunsaturated fatty acids of the phospholipid matrix of the delivery system and reduced l‐glutathione. In vivo it was demonstrated that the new nanoform of chlorin e6 provides more accumulation of PSs in tumor tissue than its free form. Moreover, its accumulation in the skin was lower and its elimination from the skin almost five times faster than when administered in free form. The observed differences of this new nanoform of chlorin e6 should lead to enhancement of antitumor efficacy and a decrease in phototoxicity. John Wiley and Sons Inc. 2018-01-02 /pmc/articles/PMC5794465/ /pubmed/29435410 http://dx.doi.org/10.1002/2211-5463.12359 Text en © 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kostryukova, Lyubov V.
Prozorovskiy, Vladimir N.
Medvedeva, Natalya V.
Ipatova, Olga M.
Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title_full Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title_fullStr Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title_full_unstemmed Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title_short Comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
title_sort comparison of a new nanoform of the photosensitizer chlorin e6, based on plant phospholipids, with its free form
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794465/
https://www.ncbi.nlm.nih.gov/pubmed/29435410
http://dx.doi.org/10.1002/2211-5463.12359
work_keys_str_mv AT kostryukovalyubovv comparisonofanewnanoformofthephotosensitizerchlorine6basedonplantphospholipidswithitsfreeform
AT prozorovskiyvladimirn comparisonofanewnanoformofthephotosensitizerchlorine6basedonplantphospholipidswithitsfreeform
AT medvedevanatalyav comparisonofanewnanoformofthephotosensitizerchlorine6basedonplantphospholipidswithitsfreeform
AT ipatovaolgam comparisonofanewnanoformofthephotosensitizerchlorine6basedonplantphospholipidswithitsfreeform