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Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine
Purinergic receptors play an important role in regulating gastrointestinal (GI) motility. Interstitial cells of Cajal (ICCs) are pacemaker cells that regulate GI smooth muscle activity. We studied the functional roles of external adenosine 5′-triphosphate (ATP) on pacemaker activity in cultured ICCs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Chonnam National University Medical School
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794481/ https://www.ncbi.nlm.nih.gov/pubmed/29399568 http://dx.doi.org/10.4068/cmj.2018.54.1.63 |
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author | Park, Il Koo Kim, Jin Ho Park, Chan Guk Kim, Man Yoo Parajuli, Shankar Prasad Hong, Chan Sik Choi, Seok Jun, Jae Yeoul |
author_facet | Park, Il Koo Kim, Jin Ho Park, Chan Guk Kim, Man Yoo Parajuli, Shankar Prasad Hong, Chan Sik Choi, Seok Jun, Jae Yeoul |
author_sort | Park, Il Koo |
collection | PubMed |
description | Purinergic receptors play an important role in regulating gastrointestinal (GI) motility. Interstitial cells of Cajal (ICCs) are pacemaker cells that regulate GI smooth muscle activity. We studied the functional roles of external adenosine 5′-triphosphate (ATP) on pacemaker activity in cultured ICCs from mouse small intestines by using the whole-cell patch clamp technique and intracellular Ca(2+) ([Ca(2+)](i)) imaging. External ATP dose-dependently depolarized the resting membrane and produced tonic inward pacemaker currents, and these effects were antagonized by suramin, a purinergic P2 receptor antagonist. ATP-induced effects on pacemaker currents were suppressed by an external Na(+)-free solution and inhibited by the nonselective cation channel blockers, flufenamic acid and niflumic acid. The removal of external Ca(2+) or treatment with thapsigargin (inhibitor of Ca(2+) uptake into endoplasmic reticulum) inhibited the ATP-induced effects on pacemaker currents. Spontaneous [Ca(2+)](i) oscillations were enhanced by external ATP. These results suggest that external ATP modulates pacemaker activity by activating nonselective cation channels via external Ca(2+) influx and [Ca(2+)](i) release from the endoplasmic reticulum. Thus, it seems that activating the purinergic P2 receptor may modulate GI motility by acting on ICCs in the small intestine. |
format | Online Article Text |
id | pubmed-5794481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Chonnam National University Medical School |
record_format | MEDLINE/PubMed |
spelling | pubmed-57944812018-02-02 Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine Park, Il Koo Kim, Jin Ho Park, Chan Guk Kim, Man Yoo Parajuli, Shankar Prasad Hong, Chan Sik Choi, Seok Jun, Jae Yeoul Chonnam Med J Original Article Purinergic receptors play an important role in regulating gastrointestinal (GI) motility. Interstitial cells of Cajal (ICCs) are pacemaker cells that regulate GI smooth muscle activity. We studied the functional roles of external adenosine 5′-triphosphate (ATP) on pacemaker activity in cultured ICCs from mouse small intestines by using the whole-cell patch clamp technique and intracellular Ca(2+) ([Ca(2+)](i)) imaging. External ATP dose-dependently depolarized the resting membrane and produced tonic inward pacemaker currents, and these effects were antagonized by suramin, a purinergic P2 receptor antagonist. ATP-induced effects on pacemaker currents were suppressed by an external Na(+)-free solution and inhibited by the nonselective cation channel blockers, flufenamic acid and niflumic acid. The removal of external Ca(2+) or treatment with thapsigargin (inhibitor of Ca(2+) uptake into endoplasmic reticulum) inhibited the ATP-induced effects on pacemaker currents. Spontaneous [Ca(2+)](i) oscillations were enhanced by external ATP. These results suggest that external ATP modulates pacemaker activity by activating nonselective cation channels via external Ca(2+) influx and [Ca(2+)](i) release from the endoplasmic reticulum. Thus, it seems that activating the purinergic P2 receptor may modulate GI motility by acting on ICCs in the small intestine. Chonnam National University Medical School 2018-01 2018-01-25 /pmc/articles/PMC5794481/ /pubmed/29399568 http://dx.doi.org/10.4068/cmj.2018.54.1.63 Text en © Chonnam Medical Journal, 2018 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Il Koo Kim, Jin Ho Park, Chan Guk Kim, Man Yoo Parajuli, Shankar Prasad Hong, Chan Sik Choi, Seok Jun, Jae Yeoul Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title | Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title_full | Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title_fullStr | Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title_full_unstemmed | Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title_short | Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine |
title_sort | effects of atp on pacemaker activity of interstitial cells of cajal from the mouse small intestine |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794481/ https://www.ncbi.nlm.nih.gov/pubmed/29399568 http://dx.doi.org/10.4068/cmj.2018.54.1.63 |
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