‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

Using CRISPR/Cas9 delivered as a RNA modality in conjunction with a lipid specifically formulated for large RNA molecules, we demonstrate that homology directed repair (HDR) rates between 20–40% can be achieved in induced pluripotent stem cells (iPSC). Furthermore, low HDR rates (between 1–20%) can...

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Detalles Bibliográficos
Autores principales: Guo, Q., Mintier, G., Ma-Edmonds, M., Storton, D., Wang, X., Xiao, X., Kienzle, B, Zhao, D., Feder, John N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794742/
https://www.ncbi.nlm.nih.gov/pubmed/29391533
http://dx.doi.org/10.1038/s41598-018-20358-5

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