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Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors

In light of the declining global malaria burden attained largely due to insecticides, a deeper understanding of the factors driving insecticide resistance is needed to mitigate its growing threat to malaria vector control programs. Following evidence of microbiota-mediated insecticide resistance in...

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Autores principales: Dada, Nsa, Sheth, Mili, Liebman, Kelly, Pinto, Jesus, Lenhart, Audrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794770/
https://www.ncbi.nlm.nih.gov/pubmed/29391526
http://dx.doi.org/10.1038/s41598-018-20367-4
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author Dada, Nsa
Sheth, Mili
Liebman, Kelly
Pinto, Jesus
Lenhart, Audrey
author_facet Dada, Nsa
Sheth, Mili
Liebman, Kelly
Pinto, Jesus
Lenhart, Audrey
author_sort Dada, Nsa
collection PubMed
description In light of the declining global malaria burden attained largely due to insecticides, a deeper understanding of the factors driving insecticide resistance is needed to mitigate its growing threat to malaria vector control programs. Following evidence of microbiota-mediated insecticide resistance in agricultural pests, we undertook a comparative study of the microbiota in mosquitoes of differing insecticide resistance status. The microbiota of wild-caught Anopheles albimanus, an important Latin American malaria vector, that were resistant (FEN_Res) or susceptible (FEN_Sus) to the organophosphate (OP) insecticide fenitrothion were characterized and compared using whole metagenome sequencing. Results showed differing composition of the microbiota and its functions between FEN_Res and FEN_Sus, with significant enrichment of OP-degrading bacteria and enzymes in FEN_Res compared to FEN_Sus. Lower bacterial diversity was observed in FEN_Res compared to FEN_Sus, suggesting the enrichment of bacterial taxa with a competitive advantage in response to insecticide selection pressure. We report and characterize for the first time whole metagenomes of An. albimanus, revealing associations between the microbiota and phenotypic resistance to the insecticide fenitrothion. This study lays the groundwork for further investigation of the role of the mosquito microbiota in insecticide resistance.
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spelling pubmed-57947702018-02-12 Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors Dada, Nsa Sheth, Mili Liebman, Kelly Pinto, Jesus Lenhart, Audrey Sci Rep Article In light of the declining global malaria burden attained largely due to insecticides, a deeper understanding of the factors driving insecticide resistance is needed to mitigate its growing threat to malaria vector control programs. Following evidence of microbiota-mediated insecticide resistance in agricultural pests, we undertook a comparative study of the microbiota in mosquitoes of differing insecticide resistance status. The microbiota of wild-caught Anopheles albimanus, an important Latin American malaria vector, that were resistant (FEN_Res) or susceptible (FEN_Sus) to the organophosphate (OP) insecticide fenitrothion were characterized and compared using whole metagenome sequencing. Results showed differing composition of the microbiota and its functions between FEN_Res and FEN_Sus, with significant enrichment of OP-degrading bacteria and enzymes in FEN_Res compared to FEN_Sus. Lower bacterial diversity was observed in FEN_Res compared to FEN_Sus, suggesting the enrichment of bacterial taxa with a competitive advantage in response to insecticide selection pressure. We report and characterize for the first time whole metagenomes of An. albimanus, revealing associations between the microbiota and phenotypic resistance to the insecticide fenitrothion. This study lays the groundwork for further investigation of the role of the mosquito microbiota in insecticide resistance. Nature Publishing Group UK 2018-02-01 /pmc/articles/PMC5794770/ /pubmed/29391526 http://dx.doi.org/10.1038/s41598-018-20367-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dada, Nsa
Sheth, Mili
Liebman, Kelly
Pinto, Jesus
Lenhart, Audrey
Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title_full Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title_fullStr Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title_full_unstemmed Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title_short Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
title_sort whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794770/
https://www.ncbi.nlm.nih.gov/pubmed/29391526
http://dx.doi.org/10.1038/s41598-018-20367-4
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