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Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical c...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794856/ https://www.ncbi.nlm.nih.gov/pubmed/29391437 http://dx.doi.org/10.1038/s41598-018-20284-6 |
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author | Kumar, Ravinder |
author_facet | Kumar, Ravinder |
author_sort | Kumar, Ravinder |
collection | PubMed |
description | 14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical comparison showed increased abundance and differential phosphorylation of these proteins in meiotic cells. Double deletion of bmh1(−/−)bmh2(−/−) leads to complete absence of sporulation with cells arrested at G(1)/S phase while further incubation of cells in sporulating media leads to cell death. In silico analysis showed the presence of 14-3-3 interacting motifs in bonafide members of kinetochore complex (KC) and spindle pole body (SPB), while present cell biological data pointed towards the possible role of yeast Bmh1/2 in regulating the behaviour of KC and SPB. We further showed the involvement of 14-3-3 in segregation of genetic material and expression of human 14-3-3β/α was able to complement the function of endogenous 14-3-3 protein even in the complex cellular process like meiosis. Our present data also established haplosufficient nature of BMH1/2. We further showed that proteins synthesized during mitotic growth enter meiotic cells without de novo synthesis except for meiotic-specific proteins required for induction and meiotic progression in Saccharomyces cerevisiae. |
format | Online Article Text |
id | pubmed-5794856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57948562018-02-12 Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast Kumar, Ravinder Sci Rep Article 14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical comparison showed increased abundance and differential phosphorylation of these proteins in meiotic cells. Double deletion of bmh1(−/−)bmh2(−/−) leads to complete absence of sporulation with cells arrested at G(1)/S phase while further incubation of cells in sporulating media leads to cell death. In silico analysis showed the presence of 14-3-3 interacting motifs in bonafide members of kinetochore complex (KC) and spindle pole body (SPB), while present cell biological data pointed towards the possible role of yeast Bmh1/2 in regulating the behaviour of KC and SPB. We further showed the involvement of 14-3-3 in segregation of genetic material and expression of human 14-3-3β/α was able to complement the function of endogenous 14-3-3 protein even in the complex cellular process like meiosis. Our present data also established haplosufficient nature of BMH1/2. We further showed that proteins synthesized during mitotic growth enter meiotic cells without de novo synthesis except for meiotic-specific proteins required for induction and meiotic progression in Saccharomyces cerevisiae. Nature Publishing Group UK 2018-02-01 /pmc/articles/PMC5794856/ /pubmed/29391437 http://dx.doi.org/10.1038/s41598-018-20284-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kumar, Ravinder Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title | Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title_full | Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title_fullStr | Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title_full_unstemmed | Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title_short | Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
title_sort | differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794856/ https://www.ncbi.nlm.nih.gov/pubmed/29391437 http://dx.doi.org/10.1038/s41598-018-20284-6 |
work_keys_str_mv | AT kumarravinder differentialabundanceandtranscriptionof1433proteinsduringvegetativegrowthandsexualreproductioninbuddingyeast |