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Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast

14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical c...

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Autor principal: Kumar, Ravinder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794856/
https://www.ncbi.nlm.nih.gov/pubmed/29391437
http://dx.doi.org/10.1038/s41598-018-20284-6
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author Kumar, Ravinder
author_facet Kumar, Ravinder
author_sort Kumar, Ravinder
collection PubMed
description 14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical comparison showed increased abundance and differential phosphorylation of these proteins in meiotic cells. Double deletion of bmh1(−/−)bmh2(−/−) leads to complete absence of sporulation with cells arrested at G(1)/S phase while further incubation of cells in sporulating media leads to cell death. In silico analysis showed the presence of 14-3-3 interacting motifs in bonafide members of kinetochore complex (KC) and spindle pole body (SPB), while present cell biological data pointed towards the possible role of yeast Bmh1/2 in regulating the behaviour of KC and SPB. We further showed the involvement of 14-3-3 in segregation of genetic material and expression of human 14-3-3β/α was able to complement the function of endogenous 14-3-3 protein even in the complex cellular process like meiosis. Our present data also established haplosufficient nature of BMH1/2. We further showed that proteins synthesized during mitotic growth enter meiotic cells without de novo synthesis except for meiotic-specific proteins required for induction and meiotic progression in Saccharomyces cerevisiae.
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spelling pubmed-57948562018-02-12 Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast Kumar, Ravinder Sci Rep Article 14-3-3 is a family of relatively low molecular weight, acidic, dimeric proteins, conserved from yeast to metazoans including humans. Apart from their role in diverse cellular processes, these proteins are also known for their role in several clinical implications. Present proteomic and biochemical comparison showed increased abundance and differential phosphorylation of these proteins in meiotic cells. Double deletion of bmh1(−/−)bmh2(−/−) leads to complete absence of sporulation with cells arrested at G(1)/S phase while further incubation of cells in sporulating media leads to cell death. In silico analysis showed the presence of 14-3-3 interacting motifs in bonafide members of kinetochore complex (KC) and spindle pole body (SPB), while present cell biological data pointed towards the possible role of yeast Bmh1/2 in regulating the behaviour of KC and SPB. We further showed the involvement of 14-3-3 in segregation of genetic material and expression of human 14-3-3β/α was able to complement the function of endogenous 14-3-3 protein even in the complex cellular process like meiosis. Our present data also established haplosufficient nature of BMH1/2. We further showed that proteins synthesized during mitotic growth enter meiotic cells without de novo synthesis except for meiotic-specific proteins required for induction and meiotic progression in Saccharomyces cerevisiae. Nature Publishing Group UK 2018-02-01 /pmc/articles/PMC5794856/ /pubmed/29391437 http://dx.doi.org/10.1038/s41598-018-20284-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kumar, Ravinder
Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title_full Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title_fullStr Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title_full_unstemmed Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title_short Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
title_sort differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794856/
https://www.ncbi.nlm.nih.gov/pubmed/29391437
http://dx.doi.org/10.1038/s41598-018-20284-6
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