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A highly efficient murine model of experimental myopia
Despite the global pandemic of myopia, the precise molecular mechanism of the onset of myopia remains largely unknown. This is partially because of the lack of efficient murine myopic models that allow genetic manipulation at low cost. Here we report a highly practical and reproducible lens-induced...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794929/ https://www.ncbi.nlm.nih.gov/pubmed/29391484 http://dx.doi.org/10.1038/s41598-018-20272-w |
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author | Jiang, Xiaoyan Kurihara, Toshihide Kunimi, Hiromitsu Miyauchi, Maki Ikeda, Shin-ichi Mori, Kiwako Tsubota, Kinya Torii, Hidemasa Tsubota, Kazuo |
author_facet | Jiang, Xiaoyan Kurihara, Toshihide Kunimi, Hiromitsu Miyauchi, Maki Ikeda, Shin-ichi Mori, Kiwako Tsubota, Kinya Torii, Hidemasa Tsubota, Kazuo |
author_sort | Jiang, Xiaoyan |
collection | PubMed |
description | Despite the global pandemic of myopia, the precise molecular mechanism of the onset of myopia remains largely unknown. This is partially because of the lack of efficient murine myopic models that allow genetic manipulation at low cost. Here we report a highly practical and reproducible lens-induced myopia model by specially designed frames and lenses for mice. A lens power dependent myopic induction in mice was shown until minus 30 diopter lenses. The phenotype was significantly stronger than form-deprivation myopia. We presented the protocol for precise evaluations of the state of myopia, including refraction, corneal curvature and axial length using up-to-date devices. We also found that myopic mouse eyes showed decreased visual acuity on optokinetic response examination. Finally, we confirmed the anti-myopic effect of 1% atropine using this model, which showed its potential in drug screening. The strong phenotype, stable evaluation and the potential for gene manipulation utilizing the presented method in mice will accelerate the translational research of myopia. |
format | Online Article Text |
id | pubmed-5794929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57949292018-02-12 A highly efficient murine model of experimental myopia Jiang, Xiaoyan Kurihara, Toshihide Kunimi, Hiromitsu Miyauchi, Maki Ikeda, Shin-ichi Mori, Kiwako Tsubota, Kinya Torii, Hidemasa Tsubota, Kazuo Sci Rep Article Despite the global pandemic of myopia, the precise molecular mechanism of the onset of myopia remains largely unknown. This is partially because of the lack of efficient murine myopic models that allow genetic manipulation at low cost. Here we report a highly practical and reproducible lens-induced myopia model by specially designed frames and lenses for mice. A lens power dependent myopic induction in mice was shown until minus 30 diopter lenses. The phenotype was significantly stronger than form-deprivation myopia. We presented the protocol for precise evaluations of the state of myopia, including refraction, corneal curvature and axial length using up-to-date devices. We also found that myopic mouse eyes showed decreased visual acuity on optokinetic response examination. Finally, we confirmed the anti-myopic effect of 1% atropine using this model, which showed its potential in drug screening. The strong phenotype, stable evaluation and the potential for gene manipulation utilizing the presented method in mice will accelerate the translational research of myopia. Nature Publishing Group UK 2018-02-01 /pmc/articles/PMC5794929/ /pubmed/29391484 http://dx.doi.org/10.1038/s41598-018-20272-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jiang, Xiaoyan Kurihara, Toshihide Kunimi, Hiromitsu Miyauchi, Maki Ikeda, Shin-ichi Mori, Kiwako Tsubota, Kinya Torii, Hidemasa Tsubota, Kazuo A highly efficient murine model of experimental myopia |
title | A highly efficient murine model of experimental myopia |
title_full | A highly efficient murine model of experimental myopia |
title_fullStr | A highly efficient murine model of experimental myopia |
title_full_unstemmed | A highly efficient murine model of experimental myopia |
title_short | A highly efficient murine model of experimental myopia |
title_sort | highly efficient murine model of experimental myopia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794929/ https://www.ncbi.nlm.nih.gov/pubmed/29391484 http://dx.doi.org/10.1038/s41598-018-20272-w |
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