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Age-related changes in function and gene expression of the male and female mouse bladder
We investigated age-related changes in in vivo and in vitro functions and gene expression of the bladder of male and female mice. Mature and aged (12 and 27–30 month old) C57BL/6 mice of both sexes were used. Frequency volume, conscious free-moving cystometry and detrusor contractile and relaxant pr...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794976/ https://www.ncbi.nlm.nih.gov/pubmed/29391518 http://dx.doi.org/10.1038/s41598-018-20406-0 |
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author | Kamei, Jun Ito, Hiroki Aizawa, Naoki Hotta, Harumi Kojima, Toshio Fujita, Yasunori Ito, Masafumi Homma, Yukio Igawa, Yasuhiko |
author_facet | Kamei, Jun Ito, Hiroki Aizawa, Naoki Hotta, Harumi Kojima, Toshio Fujita, Yasunori Ito, Masafumi Homma, Yukio Igawa, Yasuhiko |
author_sort | Kamei, Jun |
collection | PubMed |
description | We investigated age-related changes in in vivo and in vitro functions and gene expression of the bladder of male and female mice. Mature and aged (12 and 27–30 month old) C57BL/6 mice of both sexes were used. Frequency volume, conscious free-moving cystometry and detrusor contractile and relaxant properties in in vitro organ bath were evaluated. mRNA expression level of muscarinic, purinergic, and β-adrenergic receptors and gene expression changes by cDNA microarray analysis of the bladder were determined. Cystometry demonstrated storage and voiding dysfunctions with ageing in both sexes. Detrusor strips from aged mice showed weaker contractile responses particularly in the cholinergic component and weaker relaxant responses to isoproterenol. These age-related impairments were generally severer in males. mRNA expression of bladder tissue was decreased for M3 muscarinic receptors in aged males and β2-adrenoceptors in aged females. cDNA microarray analysis results, albeit substantial sex difference, indicated “cell-to-cell signaling and interaction” as the most common feature of age-related gene expression. In summary, aged mice demonstrated voiding and storage dysfunctions resembling to detrusor hyperactivity with impaired contractility (DHIC), which were more pronounced in males. Genomic changes associated with aging may contribute to the age-related bladder functional deterioration in mice. |
format | Online Article Text |
id | pubmed-5794976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57949762018-02-12 Age-related changes in function and gene expression of the male and female mouse bladder Kamei, Jun Ito, Hiroki Aizawa, Naoki Hotta, Harumi Kojima, Toshio Fujita, Yasunori Ito, Masafumi Homma, Yukio Igawa, Yasuhiko Sci Rep Article We investigated age-related changes in in vivo and in vitro functions and gene expression of the bladder of male and female mice. Mature and aged (12 and 27–30 month old) C57BL/6 mice of both sexes were used. Frequency volume, conscious free-moving cystometry and detrusor contractile and relaxant properties in in vitro organ bath were evaluated. mRNA expression level of muscarinic, purinergic, and β-adrenergic receptors and gene expression changes by cDNA microarray analysis of the bladder were determined. Cystometry demonstrated storage and voiding dysfunctions with ageing in both sexes. Detrusor strips from aged mice showed weaker contractile responses particularly in the cholinergic component and weaker relaxant responses to isoproterenol. These age-related impairments were generally severer in males. mRNA expression of bladder tissue was decreased for M3 muscarinic receptors in aged males and β2-adrenoceptors in aged females. cDNA microarray analysis results, albeit substantial sex difference, indicated “cell-to-cell signaling and interaction” as the most common feature of age-related gene expression. In summary, aged mice demonstrated voiding and storage dysfunctions resembling to detrusor hyperactivity with impaired contractility (DHIC), which were more pronounced in males. Genomic changes associated with aging may contribute to the age-related bladder functional deterioration in mice. Nature Publishing Group UK 2018-02-01 /pmc/articles/PMC5794976/ /pubmed/29391518 http://dx.doi.org/10.1038/s41598-018-20406-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kamei, Jun Ito, Hiroki Aizawa, Naoki Hotta, Harumi Kojima, Toshio Fujita, Yasunori Ito, Masafumi Homma, Yukio Igawa, Yasuhiko Age-related changes in function and gene expression of the male and female mouse bladder |
title | Age-related changes in function and gene expression of the male and female mouse bladder |
title_full | Age-related changes in function and gene expression of the male and female mouse bladder |
title_fullStr | Age-related changes in function and gene expression of the male and female mouse bladder |
title_full_unstemmed | Age-related changes in function and gene expression of the male and female mouse bladder |
title_short | Age-related changes in function and gene expression of the male and female mouse bladder |
title_sort | age-related changes in function and gene expression of the male and female mouse bladder |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794976/ https://www.ncbi.nlm.nih.gov/pubmed/29391518 http://dx.doi.org/10.1038/s41598-018-20406-0 |
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