Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tes...

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Detalles Bibliográficos
Autores principales: Yu, Min-Ah, Jeong, Hye Won, Park, Su-Jin, Kim, Young-Il, Kwon, Hyeok-Il, Kim, Eun-Ha, Si, Young-Jae, Yu, Kwang Min, Robles, Norbert John, Han, Hae Jung, Choi, Young Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2018
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795049/
https://www.ncbi.nlm.nih.gov/pubmed/29399584
http://dx.doi.org/10.7774/cevr.2018.7.1.82
Descripción
Sumario:To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.

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