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Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System
Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding the viral patho...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795462/ https://www.ncbi.nlm.nih.gov/pubmed/29361773 http://dx.doi.org/10.3390/v10010049 |
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author | Li, Shuxuan Armstrong, Najealicka Zhao, Huan Hou, Wangheng Liu, Jian Chen, Chunye Wan, Junkai Wang, Wei Zhong, Chunlian Liu, Che Zhu, Hua Xia, Ningshao Cheng, Tong Tang, Qiyi |
author_facet | Li, Shuxuan Armstrong, Najealicka Zhao, Huan Hou, Wangheng Liu, Jian Chen, Chunye Wan, Junkai Wang, Wei Zhong, Chunlian Liu, Che Zhu, Hua Xia, Ningshao Cheng, Tong Tang, Qiyi |
author_sort | Li, Shuxuan |
collection | PubMed |
description | Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding the viral pathogenesis, transmission, teratogenic mechanisms, and virus–host interactions. However, the animals used in published investigations are mostly interferon (IFN)-compromised, either genetically or via antibody treatment. Herein, we studied ZIKV infection in IFN-competent mice using African (MR766) and Asian strains (PRVABC59 and SZ-WIV01). After testing four different species of mice, we found that BALB/c neonatal mice were resistant to ZIKV infection, that Kunming, ICR and C57BL/6 neonatal mice were fatally susceptible to ZIKV infection, and that the fatality of C57BL/6 neonates from 1 to 3 days old were in a viral dose-dependent manner. The size and weight of the brain were significantly reduced, and the ZIKV-infected mice showed neuronal symptoms such as hind-limb paralysis, tremor, and poor balance during walking. Pathologic and immunofluorescent experiments revealed that ZIKV infected different areas of the central nervous system (CNS) including gray matter, hippocampus, cerebral cortex, and spinal cord, but not olfactory bulb. Interestingly, ZIKV replicated in multiple organs and resulted in pathogenesis in liver and testis, implying that ZIKV infection may engender a high health risk in neonates by postnatal infection. In summary, we investigated ZIKV pathogenesis using an animal model that is not IFN-compromised. |
format | Online Article Text |
id | pubmed-5795462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57954622018-02-09 Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System Li, Shuxuan Armstrong, Najealicka Zhao, Huan Hou, Wangheng Liu, Jian Chen, Chunye Wan, Junkai Wang, Wei Zhong, Chunlian Liu, Che Zhu, Hua Xia, Ningshao Cheng, Tong Tang, Qiyi Viruses Article Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding the viral pathogenesis, transmission, teratogenic mechanisms, and virus–host interactions. However, the animals used in published investigations are mostly interferon (IFN)-compromised, either genetically or via antibody treatment. Herein, we studied ZIKV infection in IFN-competent mice using African (MR766) and Asian strains (PRVABC59 and SZ-WIV01). After testing four different species of mice, we found that BALB/c neonatal mice were resistant to ZIKV infection, that Kunming, ICR and C57BL/6 neonatal mice were fatally susceptible to ZIKV infection, and that the fatality of C57BL/6 neonates from 1 to 3 days old were in a viral dose-dependent manner. The size and weight of the brain were significantly reduced, and the ZIKV-infected mice showed neuronal symptoms such as hind-limb paralysis, tremor, and poor balance during walking. Pathologic and immunofluorescent experiments revealed that ZIKV infected different areas of the central nervous system (CNS) including gray matter, hippocampus, cerebral cortex, and spinal cord, but not olfactory bulb. Interestingly, ZIKV replicated in multiple organs and resulted in pathogenesis in liver and testis, implying that ZIKV infection may engender a high health risk in neonates by postnatal infection. In summary, we investigated ZIKV pathogenesis using an animal model that is not IFN-compromised. MDPI 2018-01-22 /pmc/articles/PMC5795462/ /pubmed/29361773 http://dx.doi.org/10.3390/v10010049 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Shuxuan Armstrong, Najealicka Zhao, Huan Hou, Wangheng Liu, Jian Chen, Chunye Wan, Junkai Wang, Wei Zhong, Chunlian Liu, Che Zhu, Hua Xia, Ningshao Cheng, Tong Tang, Qiyi Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title | Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title_full | Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title_fullStr | Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title_full_unstemmed | Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title_short | Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System |
title_sort | zika virus fatally infects wild type neonatal mice and replicates in central nervous system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795462/ https://www.ncbi.nlm.nih.gov/pubmed/29361773 http://dx.doi.org/10.3390/v10010049 |
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