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Methods for monitoring and measurement of protein translation in time and space
Regulation of protein translation constitutes a crucial step in control of gene expression. In comparison to transcriptional regulation, however, translational control has remained a significantly under-studied layer of gene expression. This trend is now beginning to shift thanks to recent advances...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795484/ https://www.ncbi.nlm.nih.gov/pubmed/29051942 http://dx.doi.org/10.1039/c7mb00476a |
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author | Dermit, Maria Dodel, Martin Mardakheh, Faraz K. |
author_facet | Dermit, Maria Dodel, Martin Mardakheh, Faraz K. |
author_sort | Dermit, Maria |
collection | PubMed |
description | Regulation of protein translation constitutes a crucial step in control of gene expression. In comparison to transcriptional regulation, however, translational control has remained a significantly under-studied layer of gene expression. This trend is now beginning to shift thanks to recent advances in next-generation sequencing, proteomics, and microscopy based methodologies which allow accurate monitoring of protein translation rates, from single target messenger RNA molecules to genome-wide scale studies. In this review, we summarize these recent advances, and discuss how they are enabling researchers to study translational regulation in a wide variety of in vitro and in vivo biological systems, with unprecedented depth and spatiotemporal resolution. |
format | Online Article Text |
id | pubmed-5795484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-57954842018-02-15 Methods for monitoring and measurement of protein translation in time and space Dermit, Maria Dodel, Martin Mardakheh, Faraz K. Mol Biosyst Chemistry Regulation of protein translation constitutes a crucial step in control of gene expression. In comparison to transcriptional regulation, however, translational control has remained a significantly under-studied layer of gene expression. This trend is now beginning to shift thanks to recent advances in next-generation sequencing, proteomics, and microscopy based methodologies which allow accurate monitoring of protein translation rates, from single target messenger RNA molecules to genome-wide scale studies. In this review, we summarize these recent advances, and discuss how they are enabling researchers to study translational regulation in a wide variety of in vitro and in vivo biological systems, with unprecedented depth and spatiotemporal resolution. Royal Society of Chemistry 2017-12-01 2017-10-12 /pmc/articles/PMC5795484/ /pubmed/29051942 http://dx.doi.org/10.1039/c7mb00476a Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Dermit, Maria Dodel, Martin Mardakheh, Faraz K. Methods for monitoring and measurement of protein translation in time and space |
title | Methods for monitoring and measurement of protein translation in time and space |
title_full | Methods for monitoring and measurement of protein translation in time and space |
title_fullStr | Methods for monitoring and measurement of protein translation in time and space |
title_full_unstemmed | Methods for monitoring and measurement of protein translation in time and space |
title_short | Methods for monitoring and measurement of protein translation in time and space |
title_sort | methods for monitoring and measurement of protein translation in time and space |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795484/ https://www.ncbi.nlm.nih.gov/pubmed/29051942 http://dx.doi.org/10.1039/c7mb00476a |
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