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Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation

The present study investigated the molecular mechanism of apoptosis and autophagy in prostate epithelial cells under androgen deprivation (AD). BPH-1 cells were divided into four groups as follows: Control (Cont), AD, autophagy inhibition (AI) and AD + AI groups. Cells in the four groups were treate...

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Autores principales: Liu, Rong-Fu, Li, Jian, Zhang, Jie, Bai, Pei-De, Yang, Yu-Feng, Li, Wei, Wu, Zhun, Zheng, Jia-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795527/
https://www.ncbi.nlm.nih.gov/pubmed/29456633
http://dx.doi.org/10.3892/etm.2018.5726
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author Liu, Rong-Fu
Li, Jian
Zhang, Jie
Bai, Pei-De
Yang, Yu-Feng
Li, Wei
Wu, Zhun
Zheng, Jia-Xin
author_facet Liu, Rong-Fu
Li, Jian
Zhang, Jie
Bai, Pei-De
Yang, Yu-Feng
Li, Wei
Wu, Zhun
Zheng, Jia-Xin
author_sort Liu, Rong-Fu
collection PubMed
description The present study investigated the molecular mechanism of apoptosis and autophagy in prostate epithelial cells under androgen deprivation (AD). BPH-1 cells were divided into four groups as follows: Control (Cont), AD, autophagy inhibition (AI) and AD + AI groups. Cells in the four groups were treated accordingly, and the level of apoptosis was subsequently measured via flow cytometry. The expression of the microtubule-associated proteins 1A/1B light chain 3 (LC3), caspase-3, poly (ADP-ribose) polymerase 1 (PARP-1) and Beclin-1 proteins of BPH-1 cells was detected at different time points following culture in androgen-deprived medium. Western blotting revealed that the basal levels of the LC3-II protein were detected at 0 h. At 4 h, LC3-II was significantly increased compared with 0 h (P<0.05). Beginning at 20 h, the expression level of the LC3-II protein decreased significantly (P<0.05). Western blotting revealed that beginning at 24 h, the expression level of the PARP-1 protein decreased significantly (P<0.001) and the cleavage fragments of the PARP-1 protein appeared. These results further imply that autophagy serves a cell protective function by mutual inhibition with apoptosis in BPH-1 cells in the removal of androgen conditions. Furthermore, the fragments of the cleaved Beclin-1 protein appeared as 35 and 37 kDa bands. Flow cytometry analysis demonstrated that the rate of cell apoptosis in the AD, AI and AD + AI groups was significantly increased compared with the Cont group (P<0.01). Compared with the AD or the AI groups individually, the rate of cell apoptosis in the AD + AI group was significantly increased (P<0.001). These findings suggest that in the early stage of AD, autophagy has a compensatory function in the cell, whereas in the whole process, autophagy and apoptosis share a mutual antagonism. The Beclin-1-C protein fragment contributed positive feedback to the process of apoptosis, which may be a potential mechanism of AD therapy. Therefore, AD and AI exhibit a synergistic effect to further improve the level of apoptosis.
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spelling pubmed-57955272018-02-16 Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation Liu, Rong-Fu Li, Jian Zhang, Jie Bai, Pei-De Yang, Yu-Feng Li, Wei Wu, Zhun Zheng, Jia-Xin Exp Ther Med Articles The present study investigated the molecular mechanism of apoptosis and autophagy in prostate epithelial cells under androgen deprivation (AD). BPH-1 cells were divided into four groups as follows: Control (Cont), AD, autophagy inhibition (AI) and AD + AI groups. Cells in the four groups were treated accordingly, and the level of apoptosis was subsequently measured via flow cytometry. The expression of the microtubule-associated proteins 1A/1B light chain 3 (LC3), caspase-3, poly (ADP-ribose) polymerase 1 (PARP-1) and Beclin-1 proteins of BPH-1 cells was detected at different time points following culture in androgen-deprived medium. Western blotting revealed that the basal levels of the LC3-II protein were detected at 0 h. At 4 h, LC3-II was significantly increased compared with 0 h (P<0.05). Beginning at 20 h, the expression level of the LC3-II protein decreased significantly (P<0.05). Western blotting revealed that beginning at 24 h, the expression level of the PARP-1 protein decreased significantly (P<0.001) and the cleavage fragments of the PARP-1 protein appeared. These results further imply that autophagy serves a cell protective function by mutual inhibition with apoptosis in BPH-1 cells in the removal of androgen conditions. Furthermore, the fragments of the cleaved Beclin-1 protein appeared as 35 and 37 kDa bands. Flow cytometry analysis demonstrated that the rate of cell apoptosis in the AD, AI and AD + AI groups was significantly increased compared with the Cont group (P<0.01). Compared with the AD or the AI groups individually, the rate of cell apoptosis in the AD + AI group was significantly increased (P<0.001). These findings suggest that in the early stage of AD, autophagy has a compensatory function in the cell, whereas in the whole process, autophagy and apoptosis share a mutual antagonism. The Beclin-1-C protein fragment contributed positive feedback to the process of apoptosis, which may be a potential mechanism of AD therapy. Therefore, AD and AI exhibit a synergistic effect to further improve the level of apoptosis. D.A. Spandidos 2018-03 2018-01-09 /pmc/articles/PMC5795527/ /pubmed/29456633 http://dx.doi.org/10.3892/etm.2018.5726 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Rong-Fu
Li, Jian
Zhang, Jie
Bai, Pei-De
Yang, Yu-Feng
Li, Wei
Wu, Zhun
Zheng, Jia-Xin
Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title_full Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title_fullStr Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title_full_unstemmed Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title_short Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
title_sort crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795527/
https://www.ncbi.nlm.nih.gov/pubmed/29456633
http://dx.doi.org/10.3892/etm.2018.5726
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