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Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia
A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non-obstructive azoospermia. A high-thr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795641/ https://www.ncbi.nlm.nih.gov/pubmed/29456693 http://dx.doi.org/10.3892/etm.2018.5778 |
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author | Chen, Houyang Luo, Tao Chen, Tingtao Wang, Gongxian |
author_facet | Chen, Houyang Luo, Tao Chen, Tingtao Wang, Gongxian |
author_sort | Chen, Houyang |
collection | PubMed |
description | A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non-obstructive azoospermia. A high-throughput sequencing method was adopted to sequence genomic DNA extracted from the semen of healthy people (C group), patients with obstructive azoospermia (OA group) and patients with non-obstructive azoospermia (NOA group). The results revealed that Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria species comprised the majority of bacteria in the C (98.14%), OA (98.26%) and NOA (90.96%) groups. Patients in the OA and NOA groups exhibited an increase in Bacteroidetes and Firmicutes, whereas the number of Proteobacteria and Actinobacteria were decreased compared with the C group. A total of 398 common operational taxonomic units were identified, of which 27 belonged to the genus Lactobacillus. Furthermore, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States analysis indicated that the pathogenic species and reduced biodiversity in the semen of patients with azoospermia may result in an increased risk of metabolic, infectious and immune diseases. In the present study, the seminal microbiome of patients with obstructive or non-obstructive azoospermia was explored, which may be useful for developing novel treatments against azoospermia as well as for its diagnosis. |
format | Online Article Text |
id | pubmed-5795641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57956412018-02-16 Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia Chen, Houyang Luo, Tao Chen, Tingtao Wang, Gongxian Exp Ther Med Articles A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non-obstructive azoospermia. A high-throughput sequencing method was adopted to sequence genomic DNA extracted from the semen of healthy people (C group), patients with obstructive azoospermia (OA group) and patients with non-obstructive azoospermia (NOA group). The results revealed that Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria species comprised the majority of bacteria in the C (98.14%), OA (98.26%) and NOA (90.96%) groups. Patients in the OA and NOA groups exhibited an increase in Bacteroidetes and Firmicutes, whereas the number of Proteobacteria and Actinobacteria were decreased compared with the C group. A total of 398 common operational taxonomic units were identified, of which 27 belonged to the genus Lactobacillus. Furthermore, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States analysis indicated that the pathogenic species and reduced biodiversity in the semen of patients with azoospermia may result in an increased risk of metabolic, infectious and immune diseases. In the present study, the seminal microbiome of patients with obstructive or non-obstructive azoospermia was explored, which may be useful for developing novel treatments against azoospermia as well as for its diagnosis. D.A. Spandidos 2018-03 2018-01-19 /pmc/articles/PMC5795641/ /pubmed/29456693 http://dx.doi.org/10.3892/etm.2018.5778 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Chen, Houyang Luo, Tao Chen, Tingtao Wang, Gongxian Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title | Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title_full | Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title_fullStr | Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title_full_unstemmed | Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title_short | Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
title_sort | seminal bacterial composition in patients with obstructive and non-obstructive azoospermia |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795641/ https://www.ncbi.nlm.nih.gov/pubmed/29456693 http://dx.doi.org/10.3892/etm.2018.5778 |
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