Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva

The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants...

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Detalles Bibliográficos
Autores principales: Rabe, Alexander, Gesell Salazar, Manuela, Fuchs, Stephan, Kocher, Thomas, Völker, Uwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795648/
https://www.ncbi.nlm.nih.gov/pubmed/29410770
http://dx.doi.org/10.1080/20002297.2018.1428006
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author Rabe, Alexander
Gesell Salazar, Manuela
Fuchs, Stephan
Kocher, Thomas
Völker, Uwe
author_facet Rabe, Alexander
Gesell Salazar, Manuela
Fuchs, Stephan
Kocher, Thomas
Völker, Uwe
author_sort Rabe, Alexander
collection PubMed
description The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants and present a workflow including PG preparation which is suitable for metaproteomics.
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spelling pubmed-57956482018-02-06 Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva Rabe, Alexander Gesell Salazar, Manuela Fuchs, Stephan Kocher, Thomas Völker, Uwe J Oral Microbiol Notes The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants and present a workflow including PG preparation which is suitable for metaproteomics. Taylor & Francis 2018-01-24 /pmc/articles/PMC5795648/ /pubmed/29410770 http://dx.doi.org/10.1080/20002297.2018.1428006 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Notes
Rabe, Alexander
Gesell Salazar, Manuela
Fuchs, Stephan
Kocher, Thomas
Völker, Uwe
Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_full Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_fullStr Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_full_unstemmed Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_short Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_sort comparative analysis of salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
topic Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795648/
https://www.ncbi.nlm.nih.gov/pubmed/29410770
http://dx.doi.org/10.1080/20002297.2018.1428006
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