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Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro

The present study depicted the role of silicon in limiting the hyperhydricity in shoot cultures of carnation through proteomic analysis. Four-week-old healthy shoot cultures of carnation “Purple Beauty” were sub-cultured on Murashige and Skoog medium followed with four treatments, viz. control (–Si/...

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Autores principales: Muneer, Sowbiya, Wei, Hao, Park, Yoo Gyeong, Jeong, Hai Kyoung, Jeong, Byoung Ryong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796000/
https://www.ncbi.nlm.nih.gov/pubmed/29295554
http://dx.doi.org/10.3390/ijms19010050
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author Muneer, Sowbiya
Wei, Hao
Park, Yoo Gyeong
Jeong, Hai Kyoung
Jeong, Byoung Ryong
author_facet Muneer, Sowbiya
Wei, Hao
Park, Yoo Gyeong
Jeong, Hai Kyoung
Jeong, Byoung Ryong
author_sort Muneer, Sowbiya
collection PubMed
description The present study depicted the role of silicon in limiting the hyperhydricity in shoot cultures of carnation through proteomic analysis. Four-week-old healthy shoot cultures of carnation “Purple Beauty” were sub-cultured on Murashige and Skoog medium followed with four treatments, viz. control (–Si/–Hyperhydricity), hyperhydric with no silicon treatment (–Si/+Hyperhydricity), hyperhydric with silicon treatment (+Si/+Hyperhydricity), and only silicon treated with no hyperhydricity (+Si/–Hyperhydricity). Comparing to control morphological features of hyperhydric carnations showed significantly fragile, bushy and lustrous leaf nature, while Si supply restored these effects. Proteomic investigation revealed that approximately seventy protein spots were differentially expressed under Si and/or hyperhydric treatments and were either up- or downregulated in abundance depending on their functions. Most of the identified protein spots were related to stress responses, photosynthesis, and signal transduction. Proteomic results were further confirmed through immunoblots by selecting specific proteins such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), PsaA, and PsbA. Moreover, protein–protein interaction was also performed on differentially expressed protein spots using specific bioinformatic tools. In addition, stress markers were analyzed by histochemical localization of hydrogen peroxide (H(2)O(2)) and singlet oxygen (O(2)(1–)). In addition, the ultrastructure of chloroplasts in hyperhydric leaves significantly resulted in inefficiency of thylakoid lamella with the loss of grana but were recovered in silicon supplemented leaves. The proteomic study together with physiological analysis indicated that Si has a substantial role in upholding the hyperhydricity in in vitro grown carnation shoot cultures.
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spelling pubmed-57960002018-02-09 Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro Muneer, Sowbiya Wei, Hao Park, Yoo Gyeong Jeong, Hai Kyoung Jeong, Byoung Ryong Int J Mol Sci Article The present study depicted the role of silicon in limiting the hyperhydricity in shoot cultures of carnation through proteomic analysis. Four-week-old healthy shoot cultures of carnation “Purple Beauty” were sub-cultured on Murashige and Skoog medium followed with four treatments, viz. control (–Si/–Hyperhydricity), hyperhydric with no silicon treatment (–Si/+Hyperhydricity), hyperhydric with silicon treatment (+Si/+Hyperhydricity), and only silicon treated with no hyperhydricity (+Si/–Hyperhydricity). Comparing to control morphological features of hyperhydric carnations showed significantly fragile, bushy and lustrous leaf nature, while Si supply restored these effects. Proteomic investigation revealed that approximately seventy protein spots were differentially expressed under Si and/or hyperhydric treatments and were either up- or downregulated in abundance depending on their functions. Most of the identified protein spots were related to stress responses, photosynthesis, and signal transduction. Proteomic results were further confirmed through immunoblots by selecting specific proteins such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), PsaA, and PsbA. Moreover, protein–protein interaction was also performed on differentially expressed protein spots using specific bioinformatic tools. In addition, stress markers were analyzed by histochemical localization of hydrogen peroxide (H(2)O(2)) and singlet oxygen (O(2)(1–)). In addition, the ultrastructure of chloroplasts in hyperhydric leaves significantly resulted in inefficiency of thylakoid lamella with the loss of grana but were recovered in silicon supplemented leaves. The proteomic study together with physiological analysis indicated that Si has a substantial role in upholding the hyperhydricity in in vitro grown carnation shoot cultures. MDPI 2017-12-24 /pmc/articles/PMC5796000/ /pubmed/29295554 http://dx.doi.org/10.3390/ijms19010050 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muneer, Sowbiya
Wei, Hao
Park, Yoo Gyeong
Jeong, Hai Kyoung
Jeong, Byoung Ryong
Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title_full Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title_fullStr Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title_full_unstemmed Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title_short Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro
title_sort proteomic analysis reveals the dynamic role of silicon in alleviation of hyperhydricity in carnation grown in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796000/
https://www.ncbi.nlm.nih.gov/pubmed/29295554
http://dx.doi.org/10.3390/ijms19010050
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