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Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796002/ https://www.ncbi.nlm.nih.gov/pubmed/29295562 http://dx.doi.org/10.3390/ijms19010052 |
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author | de Almeida, Bruna Cristine Garcia, Natalia Maffazioli, Giovana Gonzalez dos Anjos, Laura Chada Baracat, Edmund Candido Carvalho, Katia |
author_facet | de Almeida, Bruna Cristine Garcia, Natalia Maffazioli, Giovana Gonzalez dos Anjos, Laura Chada Baracat, Edmund Candido Carvalho, Katia |
author_sort | de Almeida, Bruna Cristine |
collection | PubMed |
description | MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM. Eight miRNAs were overexpressed, and five were underexpressed in ULM. In ULMS cells, five miRNAs exhibited an overexpression and eight were down-regulated. Six miRNAs (miR-1-3p, miR-130b-3p, miR-140-5p, miR-202-3p, miR-205-5p, and miR-7-5p) presented a similar expression pattern in cell lines compared to patient samples. Of these, only three miRNAs showed significant expression in ULM (miR-1-3p, miR-140-5p, and miR-7-5p) and ULMS (miR-1-3p, miR-202-3p, and miR-7-5p). Our preliminary approach identified 24 oncomirs with an altered expression profile in ULM and ULMS cells. We identified four differentially expressed miRNAs with the same profile when compared with patients’ samples, which strongly interacted with relevant genes, including apoptosis regulator (BCL2), epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), insulin like growth factor 1 receptor (IGF1R),serine/threonine kinase (RAF1), receptor tyrosine kinase (MET), and bHLH transcription factor (MYCN). This led to alterations in their mRNA-target. |
format | Online Article Text |
id | pubmed-5796002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57960022018-02-09 Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells de Almeida, Bruna Cristine Garcia, Natalia Maffazioli, Giovana Gonzalez dos Anjos, Laura Chada Baracat, Edmund Candido Carvalho, Katia Int J Mol Sci Article MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM. Eight miRNAs were overexpressed, and five were underexpressed in ULM. In ULMS cells, five miRNAs exhibited an overexpression and eight were down-regulated. Six miRNAs (miR-1-3p, miR-130b-3p, miR-140-5p, miR-202-3p, miR-205-5p, and miR-7-5p) presented a similar expression pattern in cell lines compared to patient samples. Of these, only three miRNAs showed significant expression in ULM (miR-1-3p, miR-140-5p, and miR-7-5p) and ULMS (miR-1-3p, miR-202-3p, and miR-7-5p). Our preliminary approach identified 24 oncomirs with an altered expression profile in ULM and ULMS cells. We identified four differentially expressed miRNAs with the same profile when compared with patients’ samples, which strongly interacted with relevant genes, including apoptosis regulator (BCL2), epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), insulin like growth factor 1 receptor (IGF1R),serine/threonine kinase (RAF1), receptor tyrosine kinase (MET), and bHLH transcription factor (MYCN). This led to alterations in their mRNA-target. MDPI 2017-12-25 /pmc/articles/PMC5796002/ /pubmed/29295562 http://dx.doi.org/10.3390/ijms19010052 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Almeida, Bruna Cristine Garcia, Natalia Maffazioli, Giovana Gonzalez dos Anjos, Laura Chada Baracat, Edmund Candido Carvalho, Katia Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title | Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title_full | Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title_fullStr | Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title_full_unstemmed | Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title_short | Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells |
title_sort | oncomirs expression profiling in uterine leiomyosarcoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796002/ https://www.ncbi.nlm.nih.gov/pubmed/29295562 http://dx.doi.org/10.3390/ijms19010052 |
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