Cargando…

Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells

MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of...

Descripción completa

Detalles Bibliográficos
Autores principales: de Almeida, Bruna Cristine, Garcia, Natalia, Maffazioli, Giovana, Gonzalez dos Anjos, Laura, Chada Baracat, Edmund, Candido Carvalho, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796002/
https://www.ncbi.nlm.nih.gov/pubmed/29295562
http://dx.doi.org/10.3390/ijms19010052
_version_ 1783297409917386752
author de Almeida, Bruna Cristine
Garcia, Natalia
Maffazioli, Giovana
Gonzalez dos Anjos, Laura
Chada Baracat, Edmund
Candido Carvalho, Katia
author_facet de Almeida, Bruna Cristine
Garcia, Natalia
Maffazioli, Giovana
Gonzalez dos Anjos, Laura
Chada Baracat, Edmund
Candido Carvalho, Katia
author_sort de Almeida, Bruna Cristine
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM. Eight miRNAs were overexpressed, and five were underexpressed in ULM. In ULMS cells, five miRNAs exhibited an overexpression and eight were down-regulated. Six miRNAs (miR-1-3p, miR-130b-3p, miR-140-5p, miR-202-3p, miR-205-5p, and miR-7-5p) presented a similar expression pattern in cell lines compared to patient samples. Of these, only three miRNAs showed significant expression in ULM (miR-1-3p, miR-140-5p, and miR-7-5p) and ULMS (miR-1-3p, miR-202-3p, and miR-7-5p). Our preliminary approach identified 24 oncomirs with an altered expression profile in ULM and ULMS cells. We identified four differentially expressed miRNAs with the same profile when compared with patients’ samples, which strongly interacted with relevant genes, including apoptosis regulator (BCL2), epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), insulin like growth factor 1 receptor (IGF1R),serine/threonine kinase (RAF1), receptor tyrosine kinase (MET), and bHLH transcription factor (MYCN). This led to alterations in their mRNA-target.
format Online
Article
Text
id pubmed-5796002
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57960022018-02-09 Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells de Almeida, Bruna Cristine Garcia, Natalia Maffazioli, Giovana Gonzalez dos Anjos, Laura Chada Baracat, Edmund Candido Carvalho, Katia Int J Mol Sci Article MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM. Eight miRNAs were overexpressed, and five were underexpressed in ULM. In ULMS cells, five miRNAs exhibited an overexpression and eight were down-regulated. Six miRNAs (miR-1-3p, miR-130b-3p, miR-140-5p, miR-202-3p, miR-205-5p, and miR-7-5p) presented a similar expression pattern in cell lines compared to patient samples. Of these, only three miRNAs showed significant expression in ULM (miR-1-3p, miR-140-5p, and miR-7-5p) and ULMS (miR-1-3p, miR-202-3p, and miR-7-5p). Our preliminary approach identified 24 oncomirs with an altered expression profile in ULM and ULMS cells. We identified four differentially expressed miRNAs with the same profile when compared with patients’ samples, which strongly interacted with relevant genes, including apoptosis regulator (BCL2), epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), insulin like growth factor 1 receptor (IGF1R),serine/threonine kinase (RAF1), receptor tyrosine kinase (MET), and bHLH transcription factor (MYCN). This led to alterations in their mRNA-target. MDPI 2017-12-25 /pmc/articles/PMC5796002/ /pubmed/29295562 http://dx.doi.org/10.3390/ijms19010052 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Almeida, Bruna Cristine
Garcia, Natalia
Maffazioli, Giovana
Gonzalez dos Anjos, Laura
Chada Baracat, Edmund
Candido Carvalho, Katia
Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title_full Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title_fullStr Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title_full_unstemmed Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title_short Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells
title_sort oncomirs expression profiling in uterine leiomyosarcoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796002/
https://www.ncbi.nlm.nih.gov/pubmed/29295562
http://dx.doi.org/10.3390/ijms19010052
work_keys_str_mv AT dealmeidabrunacristine oncomirsexpressionprofilinginuterineleiomyosarcomacells
AT garcianatalia oncomirsexpressionprofilinginuterineleiomyosarcomacells
AT maffazioligiovana oncomirsexpressionprofilinginuterineleiomyosarcomacells
AT gonzalezdosanjoslaura oncomirsexpressionprofilinginuterineleiomyosarcomacells
AT chadabaracatedmund oncomirsexpressionprofilinginuterineleiomyosarcomacells
AT candidocarvalhokatia oncomirsexpressionprofilinginuterineleiomyosarcomacells