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Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis

Staphylococcus aureus is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins...

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Autores principales: Abdelmegid, Shaimaa, Murugaiyan, Jayaseelan, Abo-Ismail, Mohamed, Caswell, Jeff L., Kelton, David, Kirby, Gordon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796028/
https://www.ncbi.nlm.nih.gov/pubmed/29283389
http://dx.doi.org/10.3390/ijms19010078
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author Abdelmegid, Shaimaa
Murugaiyan, Jayaseelan
Abo-Ismail, Mohamed
Caswell, Jeff L.
Kelton, David
Kirby, Gordon M.
author_facet Abdelmegid, Shaimaa
Murugaiyan, Jayaseelan
Abo-Ismail, Mohamed
Caswell, Jeff L.
Kelton, David
Kirby, Gordon M.
author_sort Abdelmegid, Shaimaa
collection PubMed
description Staphylococcus aureus is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, milk whey samples from healthy and mastitic dairy cows were analyzed using a label-free quantitative proteomics approach. In total, 90 proteins were identified, of which 25 showed significant differential abundance between healthy and mastitic samples. In silico functional analyses indicated the involvement of the differentially abundant proteins in biological mechanisms and signaling pathways related to host defense including pathogen-recognition, direct antimicrobial function, and the acute-phase response. This proteomics and bioinformatics analysis not only facilitates the identification of putative biomarkers of S. aureus subclinical mastitis but also recapitulates previous findings demonstrating the abundance of host defense proteins in intramammary infection. All mass spectrometry data are available via ProteomeXchange with identifier PXD007516.
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spelling pubmed-57960282018-02-09 Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis Abdelmegid, Shaimaa Murugaiyan, Jayaseelan Abo-Ismail, Mohamed Caswell, Jeff L. Kelton, David Kirby, Gordon M. Int J Mol Sci Article Staphylococcus aureus is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, milk whey samples from healthy and mastitic dairy cows were analyzed using a label-free quantitative proteomics approach. In total, 90 proteins were identified, of which 25 showed significant differential abundance between healthy and mastitic samples. In silico functional analyses indicated the involvement of the differentially abundant proteins in biological mechanisms and signaling pathways related to host defense including pathogen-recognition, direct antimicrobial function, and the acute-phase response. This proteomics and bioinformatics analysis not only facilitates the identification of putative biomarkers of S. aureus subclinical mastitis but also recapitulates previous findings demonstrating the abundance of host defense proteins in intramammary infection. All mass spectrometry data are available via ProteomeXchange with identifier PXD007516. MDPI 2017-12-28 /pmc/articles/PMC5796028/ /pubmed/29283389 http://dx.doi.org/10.3390/ijms19010078 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdelmegid, Shaimaa
Murugaiyan, Jayaseelan
Abo-Ismail, Mohamed
Caswell, Jeff L.
Kelton, David
Kirby, Gordon M.
Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title_full Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title_fullStr Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title_full_unstemmed Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title_short Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
title_sort identification of host defense-related proteins using label-free quantitative proteomic analysis of milk whey from cows with staphylococcus aureus subclinical mastitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796028/
https://www.ncbi.nlm.nih.gov/pubmed/29283389
http://dx.doi.org/10.3390/ijms19010078
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