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Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration

Nanomedicine is an emerging field with great potential in disease theranostics. We generated sterically stabilized superparamagnetic iron oxide nanoparticles (s-SPIONs) with average core diameters of 10 and 25 nm and determined the in vivo biodistribution and clearance profiles. Healthy nude mice un...

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Autores principales: Pham, Binh T. T., Colvin, Emily K., Pham, Nguyen T. H., Kim, Byung J., Fuller, Emily S., Moon, Elizabeth A., Barbey, Raphael, Yuen, Samuel, Rickman, Barry H., Bryce, Nicole S., Bickley, Stephanie, Tanudji, Marcel, Jones, Stephen K., Howell, Viive M., Hawkett, Brian S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796154/
https://www.ncbi.nlm.nih.gov/pubmed/29320407
http://dx.doi.org/10.3390/ijms19010205
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author Pham, Binh T. T.
Colvin, Emily K.
Pham, Nguyen T. H.
Kim, Byung J.
Fuller, Emily S.
Moon, Elizabeth A.
Barbey, Raphael
Yuen, Samuel
Rickman, Barry H.
Bryce, Nicole S.
Bickley, Stephanie
Tanudji, Marcel
Jones, Stephen K.
Howell, Viive M.
Hawkett, Brian S.
author_facet Pham, Binh T. T.
Colvin, Emily K.
Pham, Nguyen T. H.
Kim, Byung J.
Fuller, Emily S.
Moon, Elizabeth A.
Barbey, Raphael
Yuen, Samuel
Rickman, Barry H.
Bryce, Nicole S.
Bickley, Stephanie
Tanudji, Marcel
Jones, Stephen K.
Howell, Viive M.
Hawkett, Brian S.
author_sort Pham, Binh T. T.
collection PubMed
description Nanomedicine is an emerging field with great potential in disease theranostics. We generated sterically stabilized superparamagnetic iron oxide nanoparticles (s-SPIONs) with average core diameters of 10 and 25 nm and determined the in vivo biodistribution and clearance profiles. Healthy nude mice underwent an intraperitoneal injection of these s-SPIONs at a dose of 90 mg Fe/kg body weight. Tissue iron biodistribution was monitored by atomic absorption spectroscopy and Prussian blue staining. Histopathological examination was performed to assess tissue toxicity. The 10 nm s-SPIONs resulted in higher tissue-iron levels, whereas the 25 nm s-SPIONs peaked earlier and cleared faster. Increased iron levels were detected in all organs and body fluids tested except for the brain, with notable increases in the liver, spleen, and the omentum. The tissue-iron returned to control or near control levels within 7 days post-injection, except in the omentum, which had the largest and most variable accumulation of s-SPIONs. No obvious tissue changes were noted although an influx of macrophages was observed in several tissues suggesting their involvement in s-SPION sequestration and clearance. These results demonstrate that the s-SPIONs do not degrade or aggregate in vivo and intraperitoneal administration is well tolerated, with a broad and transient biodistribution. In an ovarian tumor model, s-SPIONs were shown to accumulate in the tumors, highlighting their potential use as a chemotherapy delivery agent.
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spelling pubmed-57961542018-02-09 Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration Pham, Binh T. T. Colvin, Emily K. Pham, Nguyen T. H. Kim, Byung J. Fuller, Emily S. Moon, Elizabeth A. Barbey, Raphael Yuen, Samuel Rickman, Barry H. Bryce, Nicole S. Bickley, Stephanie Tanudji, Marcel Jones, Stephen K. Howell, Viive M. Hawkett, Brian S. Int J Mol Sci Article Nanomedicine is an emerging field with great potential in disease theranostics. We generated sterically stabilized superparamagnetic iron oxide nanoparticles (s-SPIONs) with average core diameters of 10 and 25 nm and determined the in vivo biodistribution and clearance profiles. Healthy nude mice underwent an intraperitoneal injection of these s-SPIONs at a dose of 90 mg Fe/kg body weight. Tissue iron biodistribution was monitored by atomic absorption spectroscopy and Prussian blue staining. Histopathological examination was performed to assess tissue toxicity. The 10 nm s-SPIONs resulted in higher tissue-iron levels, whereas the 25 nm s-SPIONs peaked earlier and cleared faster. Increased iron levels were detected in all organs and body fluids tested except for the brain, with notable increases in the liver, spleen, and the omentum. The tissue-iron returned to control or near control levels within 7 days post-injection, except in the omentum, which had the largest and most variable accumulation of s-SPIONs. No obvious tissue changes were noted although an influx of macrophages was observed in several tissues suggesting their involvement in s-SPION sequestration and clearance. These results demonstrate that the s-SPIONs do not degrade or aggregate in vivo and intraperitoneal administration is well tolerated, with a broad and transient biodistribution. In an ovarian tumor model, s-SPIONs were shown to accumulate in the tumors, highlighting their potential use as a chemotherapy delivery agent. MDPI 2018-01-10 /pmc/articles/PMC5796154/ /pubmed/29320407 http://dx.doi.org/10.3390/ijms19010205 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pham, Binh T. T.
Colvin, Emily K.
Pham, Nguyen T. H.
Kim, Byung J.
Fuller, Emily S.
Moon, Elizabeth A.
Barbey, Raphael
Yuen, Samuel
Rickman, Barry H.
Bryce, Nicole S.
Bickley, Stephanie
Tanudji, Marcel
Jones, Stephen K.
Howell, Viive M.
Hawkett, Brian S.
Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title_full Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title_fullStr Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title_full_unstemmed Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title_short Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration
title_sort biodistribution and clearance of stable superparamagnetic maghemite iron oxide nanoparticles in mice following intraperitoneal administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796154/
https://www.ncbi.nlm.nih.gov/pubmed/29320407
http://dx.doi.org/10.3390/ijms19010205
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