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Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper

Alkaline/neutral invertase (NINV) proteins irreversibly cleave sucrose into fructose and glucose, and play important roles in carbohydrate metabolism and plant development. To investigate the role of NINVs in the development of pepper fruits, seven NINV genes (CaNINV1–7) were identified. Phylogeneti...

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Autores principales: Shen, Long-Bin, Yao, Yuan, He, Huang, Qin, Yu-Ling, Liu, Zi-Ji, Liu, Wei-Xia, Qi, Zhi-Qiang, Yang, Li-Jia, Cao, Zhen-Mu, Yang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796173/
https://www.ncbi.nlm.nih.gov/pubmed/29324672
http://dx.doi.org/10.3390/ijms19010224
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author Shen, Long-Bin
Yao, Yuan
He, Huang
Qin, Yu-Ling
Liu, Zi-Ji
Liu, Wei-Xia
Qi, Zhi-Qiang
Yang, Li-Jia
Cao, Zhen-Mu
Yang, Yan
author_facet Shen, Long-Bin
Yao, Yuan
He, Huang
Qin, Yu-Ling
Liu, Zi-Ji
Liu, Wei-Xia
Qi, Zhi-Qiang
Yang, Li-Jia
Cao, Zhen-Mu
Yang, Yan
author_sort Shen, Long-Bin
collection PubMed
description Alkaline/neutral invertase (NINV) proteins irreversibly cleave sucrose into fructose and glucose, and play important roles in carbohydrate metabolism and plant development. To investigate the role of NINVs in the development of pepper fruits, seven NINV genes (CaNINV1–7) were identified. Phylogenetic analysis revealed that the CaNINV family could be divided into α and β groups. CaNINV1–6 had typical conserved regions and similar protein structures to the NINVs of other plants, while CaNINV7 lacked amino acid sequences at the C-terminus and N-terminus ends. An expression analysis of the CaNINV genes in different tissues demonstrated that CaNINV5 is the dominant NINV in all the examined tissues (root, stem, leaf, bud, flower, and developmental pepper fruits stage). Notably, the expression of CaNINV5 was found to gradually increase at the pre-breaker stages, followed by a decrease at the breaker stages, while it maintained a low level at the post-breaker stages. Furthermore, the invertase activity of CaNINV5 was identified by functional complementation of the invertase-deficient yeast strain SEY2102, and the optimum pH of CaNINV5 was found to be ~7.5. The gene expression and enzymatic activity of CaNINV5 suggest that it might be the main NINV enzyme for hydrolysis of sucrose during pepper fruit development.
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spelling pubmed-57961732018-02-09 Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper Shen, Long-Bin Yao, Yuan He, Huang Qin, Yu-Ling Liu, Zi-Ji Liu, Wei-Xia Qi, Zhi-Qiang Yang, Li-Jia Cao, Zhen-Mu Yang, Yan Int J Mol Sci Article Alkaline/neutral invertase (NINV) proteins irreversibly cleave sucrose into fructose and glucose, and play important roles in carbohydrate metabolism and plant development. To investigate the role of NINVs in the development of pepper fruits, seven NINV genes (CaNINV1–7) were identified. Phylogenetic analysis revealed that the CaNINV family could be divided into α and β groups. CaNINV1–6 had typical conserved regions and similar protein structures to the NINVs of other plants, while CaNINV7 lacked amino acid sequences at the C-terminus and N-terminus ends. An expression analysis of the CaNINV genes in different tissues demonstrated that CaNINV5 is the dominant NINV in all the examined tissues (root, stem, leaf, bud, flower, and developmental pepper fruits stage). Notably, the expression of CaNINV5 was found to gradually increase at the pre-breaker stages, followed by a decrease at the breaker stages, while it maintained a low level at the post-breaker stages. Furthermore, the invertase activity of CaNINV5 was identified by functional complementation of the invertase-deficient yeast strain SEY2102, and the optimum pH of CaNINV5 was found to be ~7.5. The gene expression and enzymatic activity of CaNINV5 suggest that it might be the main NINV enzyme for hydrolysis of sucrose during pepper fruit development. MDPI 2018-01-11 /pmc/articles/PMC5796173/ /pubmed/29324672 http://dx.doi.org/10.3390/ijms19010224 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shen, Long-Bin
Yao, Yuan
He, Huang
Qin, Yu-Ling
Liu, Zi-Ji
Liu, Wei-Xia
Qi, Zhi-Qiang
Yang, Li-Jia
Cao, Zhen-Mu
Yang, Yan
Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title_full Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title_fullStr Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title_full_unstemmed Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title_short Genome-Wide Identification, Expression, and Functional Analysis of the Alkaline/Neutral Invertase Gene Family in Pepper
title_sort genome-wide identification, expression, and functional analysis of the alkaline/neutral invertase gene family in pepper
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796173/
https://www.ncbi.nlm.nih.gov/pubmed/29324672
http://dx.doi.org/10.3390/ijms19010224
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