Cargando…

Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody

This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hyd...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Jian, Xu, Jing, Wu, Ping, Liao, Lingfeng, Quan, Huatao, Kang, Jing, Tian, Yongquan, Tang, Yaoyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796307/
https://www.ncbi.nlm.nih.gov/pubmed/29467877
http://dx.doi.org/10.3892/ol.2018.7729
_version_ 1783297481382035456
author He, Jian
Xu, Jing
Wu, Ping
Liao, Lingfeng
Quan, Huatao
Kang, Jing
Tian, Yongquan
Tang, Yaoyun
author_facet He, Jian
Xu, Jing
Wu, Ping
Liao, Lingfeng
Quan, Huatao
Kang, Jing
Tian, Yongquan
Tang, Yaoyun
author_sort He, Jian
collection PubMed
description This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis.
format Online
Article
Text
id pubmed-5796307
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-57963072018-02-21 Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody He, Jian Xu, Jing Wu, Ping Liao, Lingfeng Quan, Huatao Kang, Jing Tian, Yongquan Tang, Yaoyun Oncol Lett Articles This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis. D.A. Spandidos 2018-03 2018-01-04 /pmc/articles/PMC5796307/ /pubmed/29467877 http://dx.doi.org/10.3892/ol.2018.7729 Text en Copyright: © He et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
He, Jian
Xu, Jing
Wu, Ping
Liao, Lingfeng
Quan, Huatao
Kang, Jing
Tian, Yongquan
Tang, Yaoyun
Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title_full Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title_fullStr Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title_full_unstemmed Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title_short Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
title_sort rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma ebna1 antibody
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796307/
https://www.ncbi.nlm.nih.gov/pubmed/29467877
http://dx.doi.org/10.3892/ol.2018.7729
work_keys_str_mv AT hejian rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT xujing rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT wuping rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT liaolingfeng rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT quanhuatao rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT kangjing rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT tianyongquan rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody
AT tangyaoyun rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody