Cargando…
Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody
This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hyd...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796307/ https://www.ncbi.nlm.nih.gov/pubmed/29467877 http://dx.doi.org/10.3892/ol.2018.7729 |
_version_ | 1783297481382035456 |
---|---|
author | He, Jian Xu, Jing Wu, Ping Liao, Lingfeng Quan, Huatao Kang, Jing Tian, Yongquan Tang, Yaoyun |
author_facet | He, Jian Xu, Jing Wu, Ping Liao, Lingfeng Quan, Huatao Kang, Jing Tian, Yongquan Tang, Yaoyun |
author_sort | He, Jian |
collection | PubMed |
description | This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis. |
format | Online Article Text |
id | pubmed-5796307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57963072018-02-21 Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody He, Jian Xu, Jing Wu, Ping Liao, Lingfeng Quan, Huatao Kang, Jing Tian, Yongquan Tang, Yaoyun Oncol Lett Articles This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis. D.A. Spandidos 2018-03 2018-01-04 /pmc/articles/PMC5796307/ /pubmed/29467877 http://dx.doi.org/10.3892/ol.2018.7729 Text en Copyright: © He et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles He, Jian Xu, Jing Wu, Ping Liao, Lingfeng Quan, Huatao Kang, Jing Tian, Yongquan Tang, Yaoyun Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title | Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title_full | Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title_fullStr | Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title_full_unstemmed | Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title_short | Rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma EBNA1 antibody |
title_sort | rapid detection of quantum dot immune chromatography nasopharyngeal carcinoma ebna1 antibody |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796307/ https://www.ncbi.nlm.nih.gov/pubmed/29467877 http://dx.doi.org/10.3892/ol.2018.7729 |
work_keys_str_mv | AT hejian rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT xujing rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT wuping rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT liaolingfeng rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT quanhuatao rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT kangjing rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT tianyongquan rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody AT tangyaoyun rapiddetectionofquantumdotimmunechromatographynasopharyngealcarcinomaebna1antibody |